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CATH domain | Related DB codes (homologues) |
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3.40.50.720 : Rossmann fold | S00543,S00551,S00552,S00553,S00602,S00604,S00605,S00608,S00610,S00625,S00319,S00328,S00329,S00330,S00331,S00332,D00456,D00457,D00458,S00324,S00320,S00325,S00326,S00327,D00459,S00335,S00336,S00334,T00219,S00339,D00513,D00001,D00002,D00003,D00005,D00007,D00008,D00010,D00012,D00017,D00018,D00023,D00027,D00028,D00031,D00032,D00033,D00034,D00035,D00037,D00048,D00071,D00476,D00481,D00482,D00490,D00494,D00545,D00601,D00603,D00604,D00605,D00615,D00845,D00857,D00858,M00161,M00171,M00210,T00002,T00010,T00011,T00015,T00227,T00247,T00408,T00414,D00827,D00262,D00274,D00275,M00035,T00109 | 3.90.180.10 : Quinone Oxidoreductase; Chain A, domain 1 | D00001,D00002,D00018,D00048,D00481,D00482,D00490,D00615 |
Enzyme Name | UniProtKB | KEGG |
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| O57380 |
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Protein name | NADP-dependent alcohol dehydrogenase | alcohol dehydrogenase (NADP+)aldehyde reductase (NADPH2)NADP-alcohol dehydrogenaseNADP+-aldehyde reductaseNADP+-dependent aldehyde reductaseNADPH-aldehyde reductaseNADPH-dependent aldehyde reductasenonspecific succinic semialdehyde reductaseALR 1low-Km aldehyde reductasehigh-Km aldehyde reductasealcohol dehydrogenase (NADP+) |
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Synonyms | EC 1.1.1.2 |
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Pfam | PF08240 (ADH_N) PF00107 (ADH_zinc_N) [Graphical view]
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KEGG pathways | MAP code | Pathways |
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MAP00010 | Glycolysis / Gluconeogenesis | MAP00561 | Glycerolipid metabolism | MAP00930 | Caprolactam degradation |
UniProtKB:Accession Number | O57380 |
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Entry name | ADH8_RANPE |
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Activity | An alcohol + NADP(+) = an aldehyde + NADPH. |
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Subunit | Homodimer. |
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Subcellular location |
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Cofactor | Binds 2 zinc ions per subunit (By similarity). |
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References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
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[4] | p.973-975 |
| [7] | p.621-623 |
| [10] | p.80-83 |
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references | [1] |
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PubMed ID | 8068002 |
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Journal | Biochem J |
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Year | 1994 |
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Volume | 302 |
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Pages | 163-70 |
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Authors | Burdette D, Zeikus JG |
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Title | Purification of acetaldehyde dehydrogenase and alcohol dehydrogenases from Thermoanaerobacter ethanolicus 39E and characterization of the secondary-alcohol dehydrogenase (2 degrees Adh) as a bifunctional alcohol dehydrogenase--acetyl-CoA reductive thioesterase. |
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[2] |
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Comments | X-ray crystallography |
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Journal | Acta Crystallogr D Biol Crystallogr |
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Year | 1996 |
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Volume | 52 |
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Pages | 882-6 |
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Authors | Korkhin Y, Frolow F, Bogin O, Peretz M, Kalb(Gilboa) AJ, Burstein Y |
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Title | Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: Preparation, characterization and molecular symmetry. |
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Related PDB | 1kev,1ped |
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[3] |
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PubMed ID | 8639709 |
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Journal | Biochim Biophys Acta |
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Year | 1996 |
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Volume | 1294 |
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Pages | 15-24 |
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Authors | Dallet S, Legoy MD |
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Title | Hydrostatic pressure induces conformational and catalytic changes on two alcohol dehydrogenases but no oligomeric dissociation. |
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[4] |
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Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) |
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Medline ID | 98295421 |
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PubMed ID | 9836873 |
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Journal | J Mol Biol |
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Year | 1998 |
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Volume | 278 |
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Pages | 967-81 |
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Authors | Korkhin Y, Kalb(Gilboa) AJ, Peretz M, Bogin O, Burstein Y, Frolow F |
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Title | NADP-dependent bacterial alcohol dehydrogenases: crystal structure, cofactor-binding and cofactor specificity of the ADHs of Clostridium beijerinckii and Thermoanaerobacter brockii. |
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Related PDB | 1ykf |
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Related UniProtKB | P14941,P25984 |
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[5] |
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PubMed ID | 10473548 |
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Journal | J Biol Chem |
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Year | 1999 |
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Volume | 274 |
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Pages | 26021-6 |
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Authors | Peralba JM, Cederlund E, Crosas B, Moreno A, Julia P, Martinez SE, Persson B, Farr s J, Pares X, Jornvall H |
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Title | Structural and enzymatic properties of a gastric NADP(H)- dependent and retinal-active alcohol dehydrogenase. |
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Related UniProtKB | O57380 |
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[6] |
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PubMed ID | 10417229 |
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Journal | Protein Sci |
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Year | 1999 |
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Volume | 8 |
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Pages | 1241-9 |
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Authors | Korkhin Y, Kalb (Gilboa) AJ, Peretz M, Bogin O, Burstein Y, Frolow F |
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Title | Oligomeric integrity--the structural key to thermal stability in bacterial alcohol dehydrogenases. |
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[7] |
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Comments | X-ray crystallography |
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PubMed ID | 10651277 |
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Journal | Proteins |
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Year | 1999 |
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Volume | 37 |
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Pages | 619-27 |
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Authors | Li C, Heatwole J, Soelaiman S, Shoham M |
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Title | Crystal structure of a thermophilic alcohol dehydrogenase substrate complex suggests determinants of substrate specificity and thermostability. |
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Related PDB | 1bxz |
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[8] |
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Comments | X-ray crystallography |
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PubMed ID | 12381840 |
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Journal | Protein Sci |
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Year | 2002 |
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Volume | 11 |
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Pages | 2561-74 |
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Authors | Bogin O, Levin I, Hacham Y, Tel-Or S, Peretz M, Frolow F, Burstein Y |
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Title | Structural basis for the enhanced thermal stability of alcohol dehydrogenase mutants from the mesophilic bacterium Clostridium beijerinckii: contribution of salt bridging. |
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Related PDB | 1jqb |
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[9] |
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PubMed ID | 12902331 |
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Journal | J Biol Chem |
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Year | 2003 |
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Volume | 278 |
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Pages | 40573-80 |
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Authors | Rosell A, Valencia E, Ochoa WF, Fita I, Pares X, Farres J |
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Title | Complete reversal of coenzyme specificity by concerted mutation of three consecutive residues in alcohol dehydrogenase. |
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[10] |
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PubMed ID | 12818203 |
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Journal | J Mol Biol |
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Year | 2003 |
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Volume | 330 |
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Pages | 75-85 |
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Authors | Rosell A, Valencia E, Pares X, Fita I, Farres J, Ochoa WF |
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Title | Crystal structure of the vertebrate NADP(H)-dependent alcohol dehydrogenase (ADH8). |
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Related PDB | 1p0c,1p0f |
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comments | This enzyme belongs to class-IV subfamily of zinc-containing alcohol dehydrogenase family. Although this enzyme binds two zinc ions per subunit, only one zinc ion is involved in catalysis.
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created | updated |
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2004-05-24 | 2009-02-26 |
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