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CATH domain | Related DB codes (homologues) |
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2.40.10.10 : Thrombin, subunit H | M00139,D00214,M00167,D00426,M00133,D00428,D00429,D00430,D00431,D00432,D00433,D00434,D00435,M00227,M00209,D00194,D00197,D00211,D00212,D00216,M00212,D00224,M00217,M00216,D00528,D00848,D00850,D00851,D00852,D00855,M00152,M00155,M00157,M00181,M00315,M00316,M00317,M00348,M00349,T00074,T00410,T00411 |
Enzyme Name | UniProtKB | KEGG |
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| P00759 |
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Protein name | Tonin | tissue kallikreinglandular kallikreinpancreatic kallikreinsubmandibular kallikreinsubmaxillary kallikreinkidney kallikreinurinary kallikreinkallikreinsalivary kallikreinkininogeninkininogenasecallicreinglumorinpadreatinpadutinkallidinogenasebradykininogenasedepot-padutinurokallikreindilminal Donokrein P |
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Synonyms | EC 3.4.21.35Esterase 1S2 kallikreinRGK-2RSKG-5 |
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RefSeq | NP_036809.1 (Protein) NM_012677.1 (DNA/RNA sequence)
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MEROPS | S01.172 (Serine)
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Pfam | PF00089 (Trypsin) [Graphical view]
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UniProtKB:Accession Number | P00759 |
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Entry name | KLK2_RAT |
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Activity | Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa. |
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Subunit | Monomer. |
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Subcellular location |
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Cofactor | Binds 1 zinc ion per subunit. |
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Compound table: links to PDB-related databases & PoSSuM |
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| Cofactors | Substrates | Products | intermediates |
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KEGG-id | C00038 | C00017 | C00012 | C00769 | C00001 | C00012 | C01505 | C00306 | I00087 | I00085 | I00086 |
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Compound | Zinc | Protein | Peptide | Kininogen | H2O | Peptide | Kallidin | Bradykinin | Peptidyl-tetrahedral intermediate | Acyl-enzyme | Tetrahedral intermediate |
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Type | heavy metal | peptide/protein | peptide/protein | peptide/protein | H2O | peptide/protein | amino acids,amine group,aromatic ring (only carbon atom),carbohydrate,carboxyl group,imine group,lipid,peptide/protein | amino acids,amine group,aromatic ring (only carbon atom),carbohydrate,carboxyl group,imine group,lipid,peptide/protein |
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ChEBI | 29105
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| 15377
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| 6102
| 3165
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PubChem | 32051
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| 962 22247451
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| 5311111
| 439201
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1tonA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | | | |
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1tonA02 |  |  |  |  |  |  |  | Bound:_ZN | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | | | |
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references | [1] |
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Comments | X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS). |
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Medline ID | 88011252 |
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PubMed ID | 2821276 |
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Journal | J Mol Biol |
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Year | 1987 |
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Volume | 195 |
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Pages | 373-96 |
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Authors | Fujinaga M, James MN |
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Title | Rat submaxillary gland serine protease, tonin. Structure solution and refinement at 1.8 A resolution. |
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Related PDB | 1ton |
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Related UniProtKB | P00759 |
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[2] |
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PubMed ID | 8148470 |
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Journal | Drug Des Discov |
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Year | 1993 |
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Volume | 10 |
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Pages | 297-317 |
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Authors | Barth A, Wahab M, Brandt W, Frost K |
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Title | Classification of serine proteases derived from steric comparisons of their active sites. |
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[3] |
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PubMed ID | 9116171 |
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Journal | Drug Des Discov |
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Year | 1994 |
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Volume | 12 |
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Pages | 89-111 |
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Authors | Barth A, Frost K, Wahab M, Brandt W, Schadler HD, Franke R |
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Title | Classification of serine proteases derived from steric comparisons of their active sites, part II: "Ser, His, Asp arrangements in proteolytic and nonproteolytic proteins". |
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comments | This enzyme belongs to the peptidase family-S1. This enzyme has got a catalytic triad composed of Ser195/His57/Asp102, similar to that of trypsin (D00197 in EzCatDB). However, His57 orients differently from other homologous peptidases, forming a binding site for zinc ion, along with His97 and His99. Thus, the zinc ion seems to play an inhibitory role, instead of a cofactor.
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created | updated |
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2006-01-10 | 2011-02-18 |
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