EzCatDB: D00525
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DB codeD00525
RLCP classification3.105.250000.48 : Transfer
CATH domainDomain 1-.-.-.-
Domain 23.30.1360.60 : Gyrase A; domain 2Catalytic domain
E.C.2.7.1.69


Enzyme Name
UniProtKBKEGG

P69786
Protein namePTS system glucose-specific EIICB componentprotein-Npi-phosphohistidine---sugar phosphotransferase
glucose permease
PTS permease
phosphotransferase, phosphohistidinoprotein-hexose
enzyme IIl4ac
gene glC proteins
gene bglC RNA formation factors
PEP-dependent phosphotransferase enzyme II
PEP-sugar phosphotransferase enzyme II
phosphoenolpyruvate-sugar phosphotransferase enzyme II
phosphohistidinoprotein-hexose phosphotransferase
phosphohistidinoprotein-hexose phosphoribosyltransferase
phosphoprotein factor-hexose phosophotransferase
protein, specific or class, gene bglC
ribonucleic acid formation factor, gene glC
sucrose phosphotransferase system II
protein-Npi-phosphohistidine:sugar N-pros-phosphotransferase
protein-Npi-phosphohistidine:sugar Npi-phosphotransferase
SynonymsEIICB-Glc
EII-Glc
IncludesGlucose permease IIC component PTS system glucose-specific EIIC component
Glucose-specific phosphotransferase enzyme IIB component
   EC 2.7.1.69
PTS system glucose-specific EIIB component
RefSeqNP_415619.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_489369.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PfamPF00367 (PTS_EIIB)
PF02378 (PTS_EIIC)
[Graphical view]

KEGG pathways
MAP codePathways
MAP00010Glycolysis / Gluconeogenesis
MAP00051Fructose and mannose metabolism
MAP00052Galactose metabolism
MAP00053Ascorbate and aldarate metabolism
MAP00500Starch and sucrose metabolism
MAP00530Aminosugars metabolism

UniProtKB:Accession NumberP69786
Entry namePTGCB_ECOLI
ActivityProtein EIIB N(pi)-phospho-L- histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subunit
Subcellular locationCell inner membrane, Multi-pass membrane protein.
Cofactor

Compound table: links to PDB-related databases & PoSSuM

SubstratesProducts
KEGG-idC04261C11477C00615C00934
CompoundProtein N(pi)-phospho-L-histidineSugarProtein histidineSugar phosphate
Typearomatic ring (with nitrogen atoms),peptide/protein,phosphate group/phosphate ionpolysaccharidearomatic ring (with nitrogen atoms),peptide/proteinphosphate group/phosphate ion,polysaccharide
ChEBI



PubChem



            
1ibaAUnboundUnboundUnboundUnbound

Active-site residues
resource
Swiss-prot;P69786, literature [3]
pdbCatalytic residuesModified residues
          
1ibaACYS 35;ARG 38;ARG 40
CYS 35(phosphorylated)

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[2]p.7411, p.7413-7416

references
[1]
PubMed ID10736174
JournalBiochemistry
Year2000
Volume39
Pages3745-50
AuthorsBeutler R, Kaufmann M, Ruggiero F, Erni B
TitleThe glucose transporter of the Escherichia coli phosphotransferase system: linker insertion mutants and split variants.
[2]
PubMed ID9200688
JournalBiochemistry
Year1997
Volume36
Pages7408-17
AuthorsGemmecker G, Eberstadt M, Buhr A, Lanz R, Grdadolnik SG, Kessler H, Erni B
TitleGlucose transporter of Escherichia coli: NMR characterization of the phosphocysteine form of the IIB(Glc) domain and its binding interface with the IIA(Glc) subunit.
Related UniProtKBP69786
[3]
PubMed ID8784182
JournalBiochemistry
Year1996
Volume35
Pages11286-92
AuthorsEberstadt M, Grdadolnik SG, Gemmecker G, Kessler H, Buhr A, Erni B
TitleSolution structure of the IIB domain of the glucose transporter of Escherichia coli.
Related PDB1iba
Related UniProtKBP69786
[4]
PubMed ID8112346
JournalEur J Biochem
Year1994
Volume219
Pages945-52
AuthorsGolic Grdadolnik S, Eberstadt M, Gemmecker G, Kessler H, Buhr A, Erni B
TitleThe glucose transporter of Escherichia coli. Assignment of the 1H, 13C and 15N resonances and identification of the secondary structure of the soluble IIB domain.
Related UniProtKBP69786
[5]
PubMed ID8505292
JournalJ Biol Chem
Year1993
Volume268
Pages11604-9
AuthorsMeins M, Jeno P, Muller D, Richter WJ, Rosenbusch JP, Erni B
TitleCysteine phosphorylation of the glucose transporter of Escherichia coli.
Related UniProtKBP69786

comments
The same E.C. number (2.7.1.69) appears in S00297, D00527, S00283, S00420, S00046. All of them are enzymes in PTS system.
In the phosphotransferase (PTS) system, phosphoryl groups are sequentially transferred from PEP to the transported sugar. The IIB-glucose domain of IICB-glucose subunit is phosphorylated at a cysteine by IIA-glucose domain, which is phosphorylated by HPr protein. Next, the IIA domain will phosphorylate glucose. (see Fig.1 of [2])

createdupdated
2002-07-272009-03-04


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