EzCatDB: D00527
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DB codeD00527
RLCP classification3.105.230000.86 : Transfer
CATH domainDomain 13.40.50.510 : Rossmann foldCatalytic domain
Domain 23.40.35.- : Fructose Permease
E.C.2.7.1.-,2.7.1.69


Enzyme Name
UniProtKBKEGG

P69797
Protein namePTS system mannose-specific EIIAB componentprotein-Npi-phosphohistidine---sugar phosphotransferase
   (EC 2.7.1.69)

glucose permease
   (EC 2.7.1.69)

PTS permease
   (EC 2.7.1.69)

phosphotransferase, phosphohistidinoprotein-hexose
   (EC 2.7.1.69)

enzyme IIl4ac
   (EC 2.7.1.69)

gene glC proteins
   (EC 2.7.1.69)

gene bglC RNA formation factors
   (EC 2.7.1.69)

PEP-dependent phosphotransferase enzyme II
   (EC 2.7.1.69)

PEP-sugar phosphotransferase enzyme II
   (EC 2.7.1.69)

phosphoenolpyruvate-sugar phosphotransferase enzyme II
   (EC 2.7.1.69)

phosphohistidinoprotein-hexose phosphotransferase
   (EC 2.7.1.69)

phosphohistidinoprotein-hexose phosphoribosyltransferase
   (EC 2.7.1.69)

phosphoprotein factor-hexose phosophotransferase
   (EC 2.7.1.69)

protein, specific or class, gene bglC
   (EC 2.7.1.69)

ribonucleic acid formation factor, gene glC
   (EC 2.7.1.69)

sucrose phosphotransferase system II
   (EC 2.7.1.69)

protein-Npi-phosphohistidine:sugar N-pros-phosphotransferase
   (EC 2.7.1.69)

protein-Npi-phosphohistidine:sugar Npi-phosphotransferase
   (EC 2.7.1.69)

SynonymsEIIAB-Man
IncludesMannose-specific phosphotransferase enzyme IIA component
   EC 2.7.1.-
PTS system mannose-specific EIIA component EIII-Man
Mannose-specific phosphotransferase enzyme IIB component
   EC 2.7.1.69
PTS system mannose-specific EIIB component
RefSeqNP_416331.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_490078.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PfamPF03610 (EIIA-man)
PF03830 (PTSIIB_sorb)
[Graphical view]

KEGG pathways
MAP codePathwaysE.C.
MAP00010Glycolysis / Gluconeogenesis2.7.1.69
MAP00051Fructose and mannose metabolism2.7.1.69
MAP00052Galactose metabolism2.7.1.69
MAP00053Ascorbate and aldarate metabolism2.7.1.69
MAP00500Starch and sucrose metabolism2.7.1.69
MAP00530Aminosugars metabolism2.7.1.69

UniProtKB:Accession NumberP69797
Entry namePTNAB_ECOLI
ActivityProtein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L- histidine/cysteine.,Protein EIIB N(pi)-phospho-L- histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subunit
Subcellular locationCytoplasm. Cell inner membrane, Peripheral membrane protein.
Cofactor

Compound table: links to PDB-related databases & PoSSuM

SubstratesProducts
KEGG-idC02743C04261C11477L00031C00615C00934
E.C.2.7.1.-2.7.1.-,2.7.1.692.7.1.692.7.1.-2.7.1.-,2.7.1.692.7.1.69
CompoundProtein cysteineProtein N(pi)-phospho-L-histidineSugarProtein S-phosphoryl-cysteineProtein histidineSugar phosphate
Typepeptide/protein,sulfhydryl grouparomatic ring (with nitrogen atoms),peptide/protein,phosphate group/phosphate ionpolysaccharidepeptide/protein,phosphate group/phosphate ion,sulfide grouparomatic ring (with nitrogen atoms),peptide/proteinphosphate group/phosphate ion,polysaccharide
ChEBI





PubChem





              
1pdoAUnboundUnboundUnboundUnboundUnboundUnbound

Active-site residues
resource
literature [2]
pdbCatalytic residuesModified residues
          
1pdoAHIS 10;ASP 67
HIS 10(phosphorylated)

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[2]p.508-509

references
[1]
PubMed ID8131846
JournalFEBS Lett
Year1994
Volume340
Pages202-6
AuthorsMarkovic-Housley Z, Balbach J, Stolz B, Genovesio-Taverne JC
TitlePredicted topology of the N-terminal domain of the hydrophilic subunit of the mannose transporter of Escherichia coli.
Related UniProtKBP69797
[2]
PubMed ID8676384
JournalJ Mol Biol
Year1996
Volume259
Pages502-11
AuthorsNunn RS, Markovic-Housley Z, Genovesio-Taverne JC, Flukiger K, Rizkallah PJ, Jansonius JN, Schirmer T, Erni B
TitleStructure of the IIA domain of the mannose transporter from Escherichia coli at 1.7 angstroms resolution.
Related PDB1pdo
Related UniProtKBP69797
[3]
PubMed ID9074635
JournalFEBS Lett
Year1997
Volume404
Pages45-50
AuthorsGschwind RM, Gemmecker G, Leutner M, Kessler H, Gutknecht R, Lanz R, Flukiger K, Erni B
TitleSecondary structure of the IIB domain of the Escherichia coli mannose transporter, a new fold in the class of alpha/beta twisted open-sheet structures.
Related UniProtKBP69797

comments
The same E.C. number (2.7.1.69) appears in S00297, D00525, S00283, S00420. All of them are enzymes in PTS system.
In the phosphotransferase (PTS) system, a phosphoryl group is transferred from phosphoenolpyruvate (PEP) via the PTS enzymes, EI, HPr, IIA, IIB to the tranported sugar.
This enzyme is composed of IIA domain and IIB domain of a mannose transporter (IIAB-mannose).
In the IIA domain, His10 is hydrogen-bonded to a buried Asp67 that is not charge compensated. The carboxylate group of Asp67 acts as a general base and increases the basic strength of the nearby imidazole ring of His10. The nucleophilic attack of the exposed imidazole NE atom onto the phosphorus of phospho-HPr would thus be facilitated.

createdupdated
2002-07-272009-02-26


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