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| Enzyme Name | | UniProtKB | KEGG |
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| P69797 |
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| Protein name | PTS system mannose-specific EIIAB component | protein-Npi-phosphohistidine---sugar phosphotransferase (EC 2.7.1.69)glucose permease (EC 2.7.1.69)PTS permease (EC 2.7.1.69)phosphotransferase, phosphohistidinoprotein-hexose (EC 2.7.1.69)enzyme IIl4ac (EC 2.7.1.69)gene glC proteins (EC 2.7.1.69)gene bglC RNA formation factors (EC 2.7.1.69)PEP-dependent phosphotransferase enzyme II (EC 2.7.1.69)PEP-sugar phosphotransferase enzyme II (EC 2.7.1.69)phosphoenolpyruvate-sugar phosphotransferase enzyme II (EC 2.7.1.69)phosphohistidinoprotein-hexose phosphotransferase (EC 2.7.1.69)phosphohistidinoprotein-hexose phosphoribosyltransferase (EC 2.7.1.69)phosphoprotein factor-hexose phosophotransferase (EC 2.7.1.69)protein, specific or class, gene bglC (EC 2.7.1.69)ribonucleic acid formation factor, gene glC (EC 2.7.1.69)sucrose phosphotransferase system II (EC 2.7.1.69)protein-Npi-phosphohistidine:sugar N-pros-phosphotransferase (EC 2.7.1.69)protein-Npi-phosphohistidine:sugar Npi-phosphotransferase (EC 2.7.1.69) |
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| Synonyms | EIIAB-Man |
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| Includes | Mannose-specific phosphotransferase enzyme IIA component EC 2.7.1.-PTS system mannose-specific EIIA component EIII-ManMannose-specific phosphotransferase enzyme IIB component EC 2.7.1.69PTS system mannose-specific EIIB component |
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| RefSeq | NP_416331.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_490078.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
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| Pfam | PF03610 (EIIA-man)
PF03830 (PTSIIB_sorb)
[Graphical view]
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| UniProtKB:Accession Number | P69797 |
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| Entry name | PTNAB_ECOLI |
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| Activity | Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L- histidine/cysteine.,Protein EIIB N(pi)-phospho-L- histidine/cysteine + sugar = protein EIIB + sugar phosphate. |
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| Subunit |
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| Subcellular location | Cytoplasm. Cell inner membrane, Peripheral membrane protein. |
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| Cofactor |
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| Compound table: links to PDB-related databases & PoSSuM |
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| Substrates | Products |
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| KEGG-id | C02743 | C04261 | C11477 | L00031 | C00615 | C00934 |
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| E.C. | 2.7.1.- | 2.7.1.-,2.7.1.69 | 2.7.1.69 | 2.7.1.- | 2.7.1.-,2.7.1.69 | 2.7.1.69 |
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| Compound | Protein cysteine | Protein N(pi)-phospho-L-histidine | Sugar | Protein S-phosphoryl-cysteine | Protein histidine | Sugar phosphate |
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| Type | peptide/protein,sulfhydryl group | aromatic ring (with nitrogen atoms),peptide/protein,phosphate group/phosphate ion | polysaccharide | peptide/protein,phosphate group/phosphate ion,sulfide group | aromatic ring (with nitrogen atoms),peptide/protein | phosphate group/phosphate ion,polysaccharide |
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| 1pdoA |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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| Active-site residues | | resource |
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| literature [2] | | pdb | Catalytic residues | Modified residues |
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| 1pdoA | | | | | | | | HIS 10;ASP 67
| HIS 10(phosphorylated)
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| References for Catalytic Mechanism | | References | Sections | No. of steps in catalysis |
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| [2] | p.508-509 |
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| references | | [1] |
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| PubMed ID | 8131846 |
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| Journal | FEBS Lett |
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| Year | 1994 |
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| Volume | 340 |
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| Pages | 202-6 |
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| Authors | Markovic-Housley Z, Balbach J, Stolz B, Genovesio-Taverne JC |
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| Title | Predicted topology of the N-terminal domain of the hydrophilic subunit of the mannose transporter of Escherichia coli. |
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| Related UniProtKB | P69797 |
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| [2] |
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| PubMed ID | 8676384 |
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| Journal | J Mol Biol |
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| Year | 1996 |
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| Volume | 259 |
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| Pages | 502-11 |
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| Authors | Nunn RS, Markovic-Housley Z, Genovesio-Taverne JC, Flukiger K, Rizkallah PJ, Jansonius JN, Schirmer T, Erni B |
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| Title | Structure of the IIA domain of the mannose transporter from Escherichia coli at 1.7 angstroms resolution. |
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| Related PDB | 1pdo |
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| Related UniProtKB | P69797 |
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| [3] |
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| PubMed ID | 9074635 |
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| Journal | FEBS Lett |
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| Year | 1997 |
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| Volume | 404 |
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| Pages | 45-50 |
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| Authors | Gschwind RM, Gemmecker G, Leutner M, Kessler H, Gutknecht R, Lanz R, Flukiger K, Erni B |
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| Title | Secondary structure of the IIB domain of the Escherichia coli mannose transporter, a new fold in the class of alpha/beta twisted open-sheet structures. |
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| Related UniProtKB | P69797 |
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| comments | The same E.C. number (2.7.1.69) appears in S00297, D00525, S00283, S00420. All of them are enzymes in PTS system.
In the phosphotransferase (PTS) system, a phosphoryl group is transferred from phosphoenolpyruvate (PEP) via the PTS enzymes, EI, HPr, IIA, IIB to the tranported sugar.
This enzyme is composed of IIA domain and IIB domain of a mannose transporter (IIAB-mannose).
In the IIA domain, His10 is hydrogen-bonded to a buried Asp67 that is not charge compensated. The carboxylate group of Asp67 acts as a general base and increases the basic strength of the nearby imidazole ring of His10. The nucleophilic attack of the exposed imidazole NE atom onto the phosphorus of phospho-HPr would thus be facilitated.
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| created | updated |
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| 2002-07-27 | 2009-02-26 |
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