EzCatDB: D00535
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DB codeD00535
CATH domainDomain 12.60.120.200 : Jelly RollsCatalytic domain
Domain 22.60.40.- : Immunoglobulin-like
E.C.3.2.1.83

CATH domainRelated DB codes (homologues)
2.60.120.200 : Jelly RollsS00148,D00666,M00185,S00511,T00064,T00208

Enzyme Name
UniProtKBKEGG

P43478
Protein nameKappa-carrageenasekappa-carrageenase
kappa-carrageenan 4-beta-D-glycanohydrolase
SynonymsEC 3.2.1.83
PfamPF02368 (Big_2)
PF00722 (Glyco_hydro_16)
[Graphical view]
CAZyGH16 (Glycoside Hydrolase Family)


UniProtKB:Accession NumberP43478
Entry nameCGKA_ALTCA
ActivityEndohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans.
Subunit
Subcellular locationPeriplasm.
Cofactor

Compound table: links to PDB-related databases & PoSSuM

SubstratesProducts
KEGG-idC02607C00001C04839
Compoundkappa-CarrageenanH2O3-O-(3,6-Anhydro-alpha-D-galactopyranosyl)-D-galactose 4-O-sulfate
Typepolysaccharide,sulfate groupH2Opolysaccharide,sulfate group
ChEBI
15377

PubChem
962
22247451
440506
           
1dypAUnbound Unbound

Active-site residues
resource
Swiss-prot;P43478 & literature [6]
pdbCatalytic residues
         
1dypAGLU 163;ASP 165;GLU 168

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[3]p.975
[6]Fig.5, p.515-517, p.5203

references
[1]
PubMed ID2085807
JournalCarbohydr Res
Year1990
Volume208
Pages127-38
AuthorsPiculell L, Rochas C
Title87Rb+ spin relaxation in enzymically purified and in untreated iota-carrageenan.
[2]
PubMed ID1915370
JournalEur J Biochem
Year1991
Volume201
Pages241-7
AuthorsPotin P, Sanseau A, Le Gall Y, Rochas C, Kloareg B
TitlePurification and characterization of a new kappa-carrageenase from a marine Cytophaga-like bacterium.
[3]
PubMed ID7737202
JournalEur J Biochem
Year1995
Volume228
Pages971-5
AuthorsPotin P, Richard C, Barbeyron T, Henrissat B, Gey C, Petillot Y, Forest E, Dideberg O, Rochas C, Kloareg B
TitleProcessing and hydrolytic mechanism of the cgkA-encoded kappa-carrageenase of Alteromonas carrageenovora.
[4]
PubMed ID10089334
JournalActa Crystallogr D Biol Crystallogr
Year1999
Volume55
Pages918-20
AuthorsMichel G, Barbeyron T, Flament D, Vernet T, Kloareg B, Dideberg O
TitleExpression, purification, crystallization and preliminary x-ray analysis of the kappa-carrageenase from Pseudoalteromonas carrageenovora.
[5]
PubMed ID11284500
JournalCarbohydr Res
Year2001
Volume331
Pages101-6
AuthorsKnutsen SH, Sletmoen M, Kristensen T, Barbeyron T, Kloareg B, Potin P
TitleA rapid method for the separation and analysis of carrageenan oligosaccharides released by iota- and kappa -carrageenase.
[6]
CommentsX-ray crystallography
PubMed ID11435116
JournalStructure (Camb)
Year2001
Volume9
Pages513-25
AuthorsMichel G, Chantalat L, Duee E, Barbeyron T, Henrissat B, Kloareg B, Dideberg O
TitleThe kappa-carrageenase of P. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of Clan-B glycoside hydrolases.
Related PDB1dyp

comments
This enzyme belongs to the glycosidase family-16.

createdupdated
2004-03-222011-12-06


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Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005 - September 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2012 - March 2013)
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