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CATH domain | Related DB codes (homologues) |
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3.40.50.720 : Rossmann fold | S00543,S00551,S00552,S00553,S00602,S00604,S00605,S00608,S00610,S00625,S00319,S00328,S00329,S00330,S00331,S00332,D00456,D00457,D00458,S00324,S00320,S00325,S00326,S00327,D00459,S00335,S00336,S00334,T00219,S00339,D00513,D00001,D00002,D00003,D00005,D00007,D00008,D00010,D00012,D00017,D00018,D00023,D00027,D00028,D00031,D00032,D00033,D00034,D00035,D00037,D00048,D00071,D00476,D00481,D00482,D00490,D00492,D00494,D00601,D00603,D00604,D00605,D00615,D00845,D00857,D00858,M00161,M00171,M00210,T00002,T00010,T00011,T00015,T00227,T00247,T00408,T00414,D00827,D00262,D00274,D00275,M00035,T00109 |
Enzyme Name | UniProtKB | KEGG |
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| Q8I2S7 |
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Protein name |
| 3-oxoacyl-[acyl-carrier-protein] reductasebeta-ketoacyl-[acyl-carrier protein](ACP) reductasebeta-ketoacyl acyl carrier protein (ACP) reductasebeta-ketoacyl reductasebeta-ketoacyl thioester reductasebeta-ketoacyl-ACP reductasebeta-ketoacyl-acyl carrier protein reductase3-ketoacyl acyl carrier protein reductaseNADPH-specific 3-oxoacyl-[acylcarrier protein]reductase3-oxoacyl-[ACP]reductase(3R)-3-hydroxyacyl-[acyl-carrier-protein]:NADP+ oxidoreductase |
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Synonyms | 3-oxoacyl-(Acyl-carrier protein) reductase, putativeEC 1.1.1.100 |
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RefSeq | XP_001352100.1 (Protein) XM_001352064.1 (DNA/RNA sequence)
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Pfam | PF00106 (adh_short) [Graphical view]
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KEGG pathways | MAP code | Pathways |
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MAP00061 | Fatty acid biosynthesis | MAP01040 | Biosynthesis of unsaturated fatty acids |
UniProtKB:Accession Number | Q8I2S7 |
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Entry name | Q8I2S7_PLAF7 |
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Activity |
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Subunit |
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Subcellular location |
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Cofactor |
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Compound table: links to PDB-related databases & PoSSuM |
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| Substrates | Products |
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KEGG-id | C00005 | C00685 | C00080 | C00006 | C01271 |
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Compound | NADPH | 3-Oxoacyl-[acyl-carrier protein] | H+ | NADP+ | (3R)-3-Hydroxyacyl-[acyl-carrier protein] |
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Type | amide group,amine group,nucleotide | carbohydrate,peptide/protein,phosphate group/phosphate ion,sulfide group | others | amide group,amine group,nucleotide | carbohydrate,peptide/protein,phosphate group/phosphate ion,sulfide group |
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ChEBI | 16474
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| 15378
| 18009
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PubChem | 5884
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| 1038
| 5886
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| | | | | | | | | | | | |
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2c07A |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound |
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Active-site residues | resource |
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literature [15] | pdb | Catalytic residues | comment |
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| | | | | | | | | |
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2c07A |  |  |  |  |  |  |  | SER 199;TYR 212;LYS 216
| invisible 248-252
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References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
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[8] | Fig.3b, p.342 |
| [13] | p.458-459 |
| [14] | Fig.7B, p.424-425 |
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references | [1] |
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PubMed ID | 6756317 |
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Journal | Arch Biochem Biophys |
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Year | 1982 |
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Volume | 218 |
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Pages | 77-91 |
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Authors | Shimakata T, Stumpf PK |
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Title | Purification and characterizations of beta-Ketoacyl-[acyl-carrier-protein] reductase, beta-hydroxyacyl-[acylcarrier-protein] dehydrase, and enoyl-[acyl-carrier-protein] reductase from Spinacia oleracea leaves. |
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[2] |
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PubMed ID | 1562581 |
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Journal | Biochim Biophys Acta |
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Year | 1992 |
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Volume | 1120 |
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Pages | 151-9 |
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Authors | Sheldon PS, Kekwick RG, Smith CG, Sidebottom C, Slabas AR |
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Title | 3-Oxoacyl-[ACP] reductase from oilseed rape (Brassica napus). |
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[3] |
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PubMed ID | 8550484 |
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Journal | J Bacteriol |
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Year | 1996 |
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Volume | 178 |
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Pages | 571-3 |
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Authors | Shen Z, Byers DM |
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Title | Isolation of Vibrio harveyi acyl carrier protein and the fabG, acpP, and fabF genes involved in fatty acid biosynthesis. |
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[4] |
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PubMed ID | 9342868 |
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Journal | Plant Physiol |
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Year | 1997 |
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Volume | 115 |
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Pages | 501-10 |
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Authors | Xu X, Dietrich CR, Delledonne M, Xia Y, Wen TJ, Robertson DS, Nikolau BJ, Schnable PS |
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Title | Sequence analysis of the cloned glossy8 gene of maize suggests that it may code for a beta-ketoacyl reductase required for the biosynthesis of cuticular waxes. |
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[5] |
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PubMed ID | 9761917 |
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Journal | Acta Crystallogr D Biol Crystallogr |
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Year | 1998 |
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Volume | 54 |
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Pages | 427-9 |
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Authors | Rafferty JB, Fisher M, Langridge SJ, Martindale W, Thomas NC, Simon JW, Bithell S, Slabas AR, Rice DW |
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Title | Crystallization of the NADP-dependent beta-keto acyl carrier protein reductase from Escherichia coli. |
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[6] |
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PubMed ID | 10666637 |
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Journal | Acta Crystallogr D Biol Crystallogr |
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Year | 2000 |
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Volume | 56 |
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Pages | 86-8 |
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Authors | Fisher M, Sedelnikova SE, Martindale W, Thomas NC, Simon JW, Slabas AR, Rafferty JB |
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Title | Crystallization of the NADP-dependent beta-keto acyl-carrier protein reductase from Brassica napus. |
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[7] |
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PubMed ID | 10747933 |
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Journal | J Biol Chem |
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Year | 2000 |
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Volume | 275 |
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Pages | 16857-64 |
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Authors | Kremer L, Douglas JD, Baulard AR, Morehouse C, Guy MR, Alland D, Dover LG, Lakey JH, Jacobs WR Jr, Brennan PJ, Minnikin DE, Besra GS |
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Title | Thiolactomycin and related analogues as novel anti-mycobacterial agents targeting KasA and KasB condensing enzymes in Mycobacterium tuberculosis. |
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[8] |
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PubMed ID | 10801480 |
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Journal | Structure Fold Des |
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Year | 2000 |
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Volume | 8 |
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Pages | 339-47 |
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Authors | Fisher M, Kroon JT, Martindale W, Stuitje AR, Slabas AR, Rafferty JB |
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Title | The X-ray structure of Brassica napus beta-keto acyl carrier protein reductase and its implications for substrate binding and catalysis. |
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[9] |
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Comments | X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS). |
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PubMed ID | 11669613 |
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Journal | Biochemistry |
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Year | 2001 |
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Volume | 40 |
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Pages | 12772-81 |
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Authors | Price AC, Zhang YM, Rock CO, White SW |
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Title | Structure of beta-ketoacyl-[acyl carrier protein] reductase from Escherichia coli: negative cooperativity and its structural basis. |
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Related PDB | 1i01 |
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Related UniProtKB | P0AEK2 |
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[10] |
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PubMed ID | 12079383 |
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Journal | J Mol Biol |
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Year | 2002 |
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Volume | 320 |
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Pages | 249-61 |
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Authors | Cohen-Gonsaud M, Ducasse S, Hoh F, Zerbib D, Labesse G, Quemard A |
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Title | Crystal structure of MabA from Mycobacterium tuberculosis, a reductase involved in long-chain fatty acid biosynthesis. |
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[11] |
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PubMed ID | 11932442 |
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Journal | Microbiology |
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Year | 2002 |
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Volume | 148 |
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Pages | 951-60 |
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Authors | Marrakchi H, Ducasse S, Labesse G, Montrozier H, Margeat E, Emorine L, Charpentier X, Daffe M, Quemard A |
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Title | MabA (FabG1), a Mycobacterium tuberculosis protein involved in the long-chain fatty acid elongation system FAS-II. |
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[12] |
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PubMed ID | 14527946 |
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Journal | J Biol Chem |
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Year | 2003 |
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Volume | 278 |
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Pages | 52935-43 |
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Authors | Zhang YM, Wu B, Zheng J, Rock CO |
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Title | Key residues responsible for acyl carrier protein and beta-ketoacyl-acyl carrier protein reductase (FabG) interaction. |
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[13] |
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PubMed ID | 12524453 |
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Journal | Proc Natl Acad Sci U S A |
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Year | 2003 |
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Volume | 100 |
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Pages | 455-60 |
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Authors | Yang JK, Park MS, Waldo GS, Suh SW |
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Title | Directed evolution approach to a structural genomics project: Rv2002 from Mycobacterium tuberculosis. |
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[14] |
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Comments | X-ray crystallography |
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PubMed ID | 15016358 |
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Journal | Structure (Camb) |
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Year | 2004 |
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Volume | 12 |
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Pages | 417-28 |
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Authors | Price AC, Zhang YM, Rock CO, White SW |
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Title | Cofactor-induced conformational rearrangements establish a catalytically competent active site and a proton relay conduit in FabG. |
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Related PDB | 1q7b,1q7c |
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[15] |
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PubMed ID | 16225460 |
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Journal | Biochem J |
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Year | 2006 |
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Volume | 393 |
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Pages | 447-57 |
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Authors | Wickramasinghe SR, Inglis KA, Urch JE, Muller S, van Aalten DM, Fairlamb AH |
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Title | Kinetic, inhibition and structural studies on 3-oxoacyl-ACP reductase from Plasmodium falciparum, a key enzyme in fatty acid biosynthesis. |
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Related PDB | 2c07 |
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comments | This enzyme is homologous to the counterpart enzymes from other bacteria (S00328 in EzCatDB). This enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily, along with Drosophia alcohol dehydrogenase (S00319 in EzCatDB). This enzyme has got a catalytic triad composed of conserved residues, Ser, Tyr, and Lys. The conformation of these residues seems to be similar to that of the homologous enzymes. Thus, the catalytic site seems to catalyze the following reaction. (A) Hydride transfer from NADPH to keto-substrate (Reduction):
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created | updated |
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2011-07-08 | 2012-06-01 |
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