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CATH domain | Related DB codes (homologues) |
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2.40.10.10 : Thrombin, subunit H | M00139,D00214,M00167,D00426,M00133,D00428,D00429,D00430,D00431,D00432,D00433,D00434,D00435,M00227,M00209,D00194,D00197,D00211,D00212,D00216,M00212,D00224,D00497,M00217,M00216,D00528,D00848,D00851,D00852,D00855,M00152,M00155,M00157,M00181,M00315,M00316,M00317,M00348,M00349,T00074,T00410,T00411 |
Enzyme Name | UniProtKB | KEGG |
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| Q61955 |
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Protein name | Kallikrein-8 | Kallikrein 8KLK8PRSS19Human kallikrein 8hK8mK8OvasinTumor-associated differentially expressed gene 14TADG-14NPNeuropsin |
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Synonyms | mK8EC 3.4.21.118NeuropsinNPSerine protease 19 |
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RefSeq | NP_032966.1 (Protein) NM_008940.2 (DNA/RNA sequence)
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MEROPS | S01.244 (Serine)
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Pfam | PF00089 (Trypsin) [Graphical view]
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UniProtKB:Accession Number | Q61955 |
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Entry name | NRPN_MOUSE |
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Activity | Cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys. |
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Subunit | Interacts with SPINK9 (By similarity). |
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Subcellular location | Secreted. Cytoplasm. Note: Shows a cytoplasmic distribution in the keratinocytes. |
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Cofactor |
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Compound table: links to PDB-related databases & PoSSuM |
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| Substrates | Products | intermediates |
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KEGG-id | C00012 | C00001 | C00012 | I00087 | I00085 | I00086 |
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Compound | Peptide | H2O | Peptide | Peptidyl-Ser-tetrahedral-intermediate (with previous peptide) | Acyl-enzyme(Peptidyl-Ser-acyl group) | Peptidyl-Ser-tetrahedral-intermediate |
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Type | peptide/protein | H2O | peptide/protein |
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ChEBI |
| 15377
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PubChem |
| 22247451 962
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1npmA01 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound |
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1npmB01 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound |
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1npmA02 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound |
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1npmB02 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound |
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References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
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[3] | Figure 5, p.4505-4508 |
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references | [1] |
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Comments | PROTEIN SEQUENCE OF N-TERMINUS, FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, MASS SPECTROMETRY. |
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PubMed ID | 9556608 |
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Journal | J Biol Chem |
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Year | 1998 |
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Volume | 273 |
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Pages | 11189-96 |
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Authors | Shimizu C, Yoshida S, Shibata M, Kato K, Momota Y, Matsumoto K, Shiosaka T, Midorikawa R, Kamachi T, Kawabe A, Shiosaka S |
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Title | Characterization of recombinant and brain neuropsin, a plasticity-related serine protease. |
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[2] |
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Comments | X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 33-257. |
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PubMed ID | 9933620 |
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Journal | J Biol Chem |
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Year | 1999 |
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Volume | 274 |
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Pages | 4220-4 |
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Authors | Kishi T, Kato M, Shimizu T, Kato K, Matsumoto K, Yoshida S, Shiosaka S, Hakoshima T |
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Title | Crystal structure of neuropsin, a hippocampal protease involved in kindling epileptogenesis. |
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Related PDB | 1npm |
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Related UniProtKB | Q61955 |
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[3] |
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PubMed ID | 12475199 |
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Journal | Chem Rev |
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Year | 2002 |
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Volume | 102 |
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Pages | 4501-24 |
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Authors | Hedstrom L |
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Title | Serine protease mechanism and specificity. |
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[4] |
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PubMed ID | 16337200 |
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Journal | FEBS Lett |
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Year | 2005 |
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Volume | 579 |
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Pages | 6879-84 |
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Authors | Rajapakse S, Ogiwara K, Takano N, Moriyama A, Takahashi T |
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Title | Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins. |
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[5] |
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PubMed ID | 20940292 |
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Journal | J Biol Chem |
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Year | 2011 |
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Volume | 286 |
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Pages | 687-706 |
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Authors | Eissa A, Amodeo V, Smith CR, Diamandis EP |
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Title | Kallikrein-related peptidase-8 (KLK8) is an active serine protease in human epidermis and sweat and is involved in a skin barrier proteolytic cascade. |
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comments | This enzyme belongs to peptidase family-S1. Since this enzyme has got the same catalytic site as trypsin, it must catalyze the trypsin-like reaction.
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created | updated |
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2011-02-16 | 2012-07-31 |
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