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CATH domain | Related DB codes (homologues) |
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2.40.10.10 : Thrombin, subunit H | M00139,D00214,M00167,D00426,M00133,D00428,D00429,D00430,D00431,D00432,D00433,D00434,D00435,M00227,M00209,D00194,D00197,D00211,D00212,D00216,M00212,D00224,D00497,M00217,M00216,D00528,D00848,D00850,D00852,D00855,M00152,M00155,M00157,M00181,M00315,M00316,M00317,M00348,M00349,T00074,T00410,T00411 |
Enzyme Name | UniProtKB | KEGG |
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| P0C1U8 | Q99V45 |
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Protein name | Glutamyl endopeptidase | Glutamyl endopeptidase | Glutamyl endopeptidaseV8 proteinaseEndoproteinase Glu-CStaphylococcal serine proteinase |
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Synonyms | EC 3.4.21.19Endoproteinase Glu-CStaphylococcal serine proteinaseV8 proteaseV8 proteinase | EC 3.4.21.19Endoproteinase Glu-CStaphylococcal serine proteinaseV8 proteaseV8 proteinase |
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RefSeq |
| NP_371572.1 (Protein) NC_002758.2 (DNA/RNA sequence)
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MEROPS | S01.269 (Serine)
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UniProtKB:Accession Number | P0C1U8 | Q99V45 |
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Entry name | SSPA_STAAU | SSPA_STAAM |
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Activity | Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa. | Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa. |
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Subunit |
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Subcellular location | Secreted. | Secreted (By similarity). |
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Cofactor |
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Compound table: links to PDB-related databases & PoSSuM |
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| Substrates | Products | intermediates |
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KEGG-id | C00017 | C00001 | C00017 | I00087 | I00085 | I00086 |
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Compound | Protein | H2O | Protein | Peptidyl-Ser-tetrahedral-intermediate (with previous peptide) | Acyl-enzyme(Peptidyl-Ser-acyl group) | Peptidyl-Ser-tetrahedral-intermediate |
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Type | peptide/protein | H2O | peptide/protein |
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ChEBI |
| 15377
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PubChem |
| 22247451 962
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1wczA01 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound |
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1qy6A01 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound |
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2o8lA01 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound |
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1wczA02 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound |
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1qy6A02 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound |
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2o8lA02 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound |
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References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
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[3] | Figure 5, p.4505-4508 |
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references | [1] |
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PubMed ID | 4509307 |
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Journal | Proc Natl Acad Sci U S A |
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Year | 1972 |
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Volume | 69 |
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Pages | 3506-9 |
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Authors | Houmard J, Drapeau GR |
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Title | Staphylococcal protease: a proteolytic enzyme specific for glutamoyl bonds. |
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[2] |
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PubMed ID | 136253 |
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Journal | Biochem Biophys Res Commun |
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Year | 1976 |
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Volume | 72 |
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Pages | 411-7 |
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Authors | Austen BM, Smith EL |
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Title | Action of staphylococcal proteinase on peptides of varying chain length and composition. |
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[3] |
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PubMed ID | 12475199 |
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Journal | Chem Rev |
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Year | 2002 |
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Volume | 102 |
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Pages | 4501-24 |
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Authors | Hedstrom L |
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Title | Serine protease mechanism and specificity. |
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[4] |
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Comments | X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 69-342. |
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PubMed ID | 14747701 |
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Journal | Acta Crystallogr D Biol Crystallogr |
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Year | 2004 |
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Volume | 60 |
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Pages | 256-9 |
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Authors | Prasad L, Leduc Y, Hayakawa K, Delbaere LT |
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Title | The structure of a universally employed enzyme: V8 protease from Staphylococcus aureus. |
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Related PDB | 1qy6,2o8l |
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Related UniProtKB | Q99V45 |
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[5] |
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PubMed ID | 17878159 |
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Journal | J Biol Chem |
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Year | 2007 |
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Volume | 282 |
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Pages | 34129-38 |
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Authors | Nickerson NN, Prasad L, Jacob L, Delbaere LT, McGavin MJ |
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Title | Activation of the SspA serine protease zymogen of Staphylococcus aureus proceeds through unique variations of a trypsinogen-like mechanism and is dependent on both autocatalytic and metalloprotease-specific processing. |
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comments | This enzyme belongs to peptidase family-S1B. Since this enzyme has got the same catalytic site as trypsin, it must catalyze the trypsin-like reaction.
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created | updated |
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2011-02-24 | 2012-07-31 |
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