EzCatDB: M00021
Related links:    PDB-formatted query search system Fasta-formatted query search system Fasta-formatted query search system

DB codeM00021
CATH domainDomain 11.-.-.-
Domain 2-.-.-.-
Domain 3-.-.-.-
Domain 42.60.40.350 : Immunoglobulin-like
Domain 52.60.98.10 : Tick-borne Encephalitis virus Glycoprotein; domain 1
Domain 63.30.67.10 : Viral Envelope Glycoprotein; domain 2
Domain 73.30.387.10 : Viral Envelope Glycoprotein; domain 3
Domain 8-.-.-.-
Domain 9-.-.-.-
Domain 10-.-.-.-
Domain 112.40.10.- : Thrombin, subunit H
Domain 122.40.10.- : Thrombin, subunit H
Domain 133.-.-.-
Domain 143.40.50.- : Rossmann fold
Domain 15-.-.-.-
Domain 16-.-.-.-
Domain 173.40.50.- : Rossmann fold
Domain 183.40.50.- : Rossmann fold
E.C.3.4.21.91,2.7.7.48


Enzyme Name
UniProtKBKEGG

P14336
Protein nameGenome polyproteinflavivirin
   (EC 3.4.21.91)

Yellow fever virus (flavivirus) protease
   (EC 3.4.21.91)

NS2B-3 proteinase
   (EC 3.4.21.91)

RNA-directed RNA polymerase
   (EC 2.7.7.48)

RNA nucleotidyltransferase (RNA-directed)
   (EC 2.7.7.48)

RNA nucleotidyltransferase (RNA-directed)
   (EC 2.7.7.48)

RNA-dependent ribonucleate nucleotidyltransferase
   (EC 2.7.7.48)

3D polymerase
   (EC 2.7.7.48)

PB1 proteins
   (EC 2.7.7.48)

PB2 proteins
   (EC 2.7.7.48)

phage f2 replicase
   (EC 2.7.7.48)

polymerase L
   (EC 2.7.7.48)

Q-beta replicase
   (EC 2.7.7.48)

phage f2 replicase
   (EC 2.7.7.48)

ribonucleic acid replicase
   (EC 2.7.7.48)

ribonucleic acid-dependent ribonucleate nucleotidyltransferase
   (EC 2.7.7.48)

ribonucleic acid-dependent ribonucleic acid polymerase
   (EC 2.7.7.48)

ribonucleic replicase
   (EC 2.7.7.48)

ribonucleic synthetase
   (EC 2.7.7.48)

RNA replicase
   (EC 2.7.7.48)

RNA synthetase
   (EC 2.7.7.48)

RNA transcriptase
   (EC 2.7.7.48)

RNA-dependent ribonucleate nucleotidyltransferase
   (EC 2.7.7.48)

RDRP
   (EC 2.7.7.48)

RNA-dependent RNA polymerase
   (EC 2.7.7.48)

RNA-dependent RNA replicase
   (EC 2.7.7.48)

transcriptase
   (EC 2.7.7.48)

SynonymsNone
ContainsPeptide 2k
Capsid protein C Core protein
prM
Peptide pr
Small envelope protein M Matrix protein
Envelope protein E
Non-structural protein 1
(NS1)
Non-structural protein 2A
(NS2A)
Serine protease subunit NS2B Flavivirin protease NS2B regulatory subunit Non-structural protein 2B
Serine protease NS3
   EC 3.4.21.91
   EC 3.6.1.15
   EC 3.6.4.13
Flavivirin protease NS3 catalytic subunit Non-structural protein 3
Non-structural protein 4A
(NS4A)
Non-structural protein 4B
(NS4B)
RNA-directed RNA polymerase NS5
   EC 2.1.1.56
   EC 2.1.1.57
   EC 2.7.7.48
Non-structural protein 5
RefSeqNP_043135.1 (Protein)
NC_001672.1 (DNA/RNA sequence)
MEROPSS07.001 (Serine)
PfamPF01003 (Flavi_capsid)
PF07652 (Flavi_DEAD)
PF02832 (Flavi_glycop_C)
PF00869 (Flavi_glycoprot)
PF01004 (Flavi_M)
PF00948 (Flavi_NS1)
PF01005 (Flavi_NS2A)
PF01002 (Flavi_NS2B)
PF01350 (Flavi_NS4A)
PF01349 (Flavi_NS4B)
PF00972 (Flavi_NS5)
PF01570 (Flavi_propep)
PF01728 (FtsJ)
PF00271 (Helicase_C)
PF00949 (Peptidase_S7)
[Graphical view]

KEGG pathways
MAP codePathwaysE.C.
MAP00230Purine metabolism2.7.7.48

UniProtKB:Accession NumberP14336
Entry namePOLG_TBEVW
ActivitySelective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.,Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
SubunitNS3 and NS2B form a heterodimer. NS3 is the catalytic subunit, whereas NS2B strongly stimulates the latter (By similarity).
Subcellular locationProtein C: Virion (Potential). Membrane, Single-pass membrane protein (Potential).,Small envelope protein M: Virion (Potential). Membrane, Single-pass membrane protein (Potential).,Envelope protein E: Virion (Potential). Membrane, Multi-pass membrane protein (Potential).,Non-structural protein 4A: Membrane, Single- pass membrane protein (Potential).,Non-structural protein 4B: Membrane, Multi- pass membrane protein (Potential).
Cofactor

Compound table: links to PDB-related databases & PoSSuM

SubstratesProducts
KEGG-idC00017C00201C00046C00017C00012C00013C00046
E.C.3.4.21.912.7.7.482.7.7.483.4.21.913.4.21.912.7.7.482.7.7.48
CompoundProteinNucleoside triphosphateRNA(n)ProteinPeptidePyrophosphateRNA(n+1)
Typepeptide/proteinnucleotidenucleic acidspeptide/proteinpeptide/proteinphosphate group/phosphate ionnucleic acids
ChEBI




29888

PubChem




21961011
1023

               
1svbA01UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1svbA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1svbA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1svbA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound

Active-site residues
pdb
        
1svbA01
1svbA02
1svbA03
1svbA04


references
[1]
PubMed ID8591041
JournalStructure
Year1995
Volume3
Pages645-8
AuthorsStuart D, Gouet P
TitleViral envelope glycoproteins swing into action.
[2]
CommentsX-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 281-680.
Medline ID95272700
PubMed ID7753193
JournalNature
Year1995
Volume375
Pages291-8
AuthorsRey FA, Heinz FX, Mandl C, Kunz C, Harrison SC
TitleThe envelope glycoprotein from tick-borne encephalitis virus at 2 A resolution.
Related PDB1svb
Related UniProtKBP14336
[3]
PubMed ID11287576
JournalJ Virol
Year2001
Volume75
Pages4268-75
AuthorsAllison SL, Schalich J, Stiasny K, Mandl CW, Heinz FX
TitleMutational evidence for an internal fusion peptide in flavivirus envelope protein E.
[4]
PubMed ID11264392
JournalJ Virol
Year2001
Volume75
Pages4002-7
AuthorsBhardwaj S, Holbrook M, Shope RE, Barrett AD, Watowich SJ
TitleBiophysical characterization and vector-specific antagonist activity of domain III of the tick-borne flavivirus envelope protein.

comments
This protein (Swiss-prot;P14336) is genome polyprotein from Tick-borne encephalitis virus, which is composed of capsid protein C, matrix protein, major envelope protein E, four non-structural proteins, flavivirin (EC 3.4.21.91), RNA-directed RNA polymerase (EC 2.7.7.48).
However, the catalytic domain of these enzymes have not been determined yet. (The PDB structure (1svb) corresponds to major envelope protein E.)

createdupdated
2002-10-182009-02-26


Copyright: Nozomi Nagano, JST & CBRC-AIST
Funded by PRESTO/Japan Science and Technology Corporation (JST) (December 2001 - November 2004)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005 - September 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2012 - March 2013)
Supported by the commission for the Development of Artificial Gene Synthesis Technology for Creating Innovative Biomaterial from the Ministry of Economy, Trade and Industry (METI) (October 2012 - )
© Biotechnology Research Institute for Drug Discovery, AIST, 2015-2016 All Rights Reserved.
© Computational Biology Research Center, AIST, 2004-2016 All Rights Reserved.