EzCatDB: M00037
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DB codeM00037
CATH domainDomain 13.40.50.20 : Rossmann fold
Domain 23.30.1490.20 : Dna Ligase; domain 1
Domain 33.30.470.20 : D-amino Acid Aminotransferase; Chain A, domain 1
Domain 43.90.600.10 : Glycinamide Ribonucleotide Synthetase; Chain A, domain 4
E.C.6.3.4.13

CATH domainRelated DB codes (homologues)
3.30.1490.20 : Dna Ligase; domain 1T00082,M00035,T00107,T00108
3.30.470.20 : D-amino Acid Aminotransferase; Chain A, domain 1T00082,D00298,M00035,M00051,T00107,T00108
3.40.50.20 : Rossmann foldT00082,T00107,T00108

Enzyme Name
UniProtKBKEGG

P15640
Protein namePhosphoribosylamine--glycine ligasephosphoribosylamine---glycine ligase
phosphoribosylglycinamide synthetase
glycinamide ribonucleotide synthetase
phosphoribosylglycineamide synthetase
glycineamide ribonucleotide synthetase
2-amino-N-ribosylacetamide 5'-phosphate kinosynthase
5'-phosphoribosylglycinamide synthetase
GAR
SynonymsEC 6.3.4.13
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
RefSeqNP_418433.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_491455.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PfamPF01071 (GARS_A)
PF02843 (GARS_C)
PF02844 (GARS_N)
[Graphical view]

KEGG pathways
MAP codePathways
MAP00230Purine metabolism

UniProtKB:Accession NumberP15640
Entry namePUR2_ECOLI
ActivityATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
SubunitMonomer.
Subcellular location
CofactorBinds 1 magnesium or manganese ion per subunit (By similarity).

Compound table: links to PDB-related databases & PoSSuM

CofactorsSubstratesProducts
KEGG-idC00305C00002C03090C00037C00008C00009C03838
CompoundMagnesiumATP5-Phospho-D-ribosylamineGlycineADPOrthophosphateN1-(5-Phospho-D-ribosyl)glycinamide
Typedivalent metal (Ca2+, Mg2+)amine group,nucleotideamine group,carbohydrate,phosphate group/phosphate ionamino acidsamine group,nucleotidephosphate group/phosphate ionamide group,amine group,carbohydrate,phosphate group/phosphate ion
ChEBI18420
15422
37737
15428
57305
16761
26078

PubChem888
5957
439905
750
5257127
6022
22486802
1004
160913
               
1gsoA01UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1gsoA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1gsoA03UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1gsoA04UnboundUnboundUnboundUnboundUnboundUnboundUnbound

Active-site residues
pdb
        
1gsoA01
1gsoA02
1gsoA03
1gsoA04


references
[1]
PubMed ID3756144
JournalBiochemistry
Year1986
Volume25
Pages4356-65
AuthorsSchrimsher JL, Schendel FJ, Stubbe J
TitleIsolation of a multifunctional protein with aminoimidazole ribonucleotide synthetase, glycinamide ribonucleotide synthetase, and glycinamide ribonucleotide transformylase activities: characterization of aminoimidazole ribonucleotide synthetase.
[2]
PubMed ID8299947
JournalGene
Year1993
Volume137
Pages195-202
AuthorsKan JL, Jannatipour M, Taylor SM, Moran RG
TitleMouse cDNAs encoding a trifunctional protein of de novo purine synthesis and a related single-domain glycinamide ribonucleotide synthetase.
[3]
CommentsX-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS)
Medline ID99060041
PubMed ID9843369
JournalBiochemistry
Year1998
Volume37
Pages15647-62
AuthorsWang W, Kappock TJ, Stubbe J, Ealick SE
TitleX-ray crystal structure of glycinamide ribonucleotide synthetase from Escherichia coli.
Related PDB1gso
Related UniProtKBP15640
[4]
PubMed ID10089364
JournalActa Crystallogr D Biol Crystallogr
Year1999
Volume55
Pages518-21
AuthorsWeaver TM, Wang W, Ealick SE
TitlePurification, crystallization and preliminary X-ray diffraction data from selenomethionine glycinamide ribonucleotide synthetase.


createdupdated
2004-03-252009-02-26


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