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CATH domain | Related DB codes (homologues) |
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3.40.50.1220 : Rossmann fold | M00162,T00237,T00230 | 3.40.50.1770 : Rossmann fold | D00031 | 3.40.50.720 : Rossmann fold | S00543,S00551,S00552,S00553,S00602,S00604,S00605,S00608,S00610,S00625,S00319,S00328,S00329,S00330,S00331,S00332,D00456,D00457,D00458,S00324,S00320,S00325,S00326,S00327,D00459,S00335,S00336,S00334,T00219,S00339,D00513,D00001,D00002,D00003,D00005,D00007,D00008,D00010,D00012,D00017,D00018,D00023,D00027,D00028,D00031,D00032,D00033,D00034,D00035,D00037,D00048,D00071,D00476,D00481,D00482,D00490,D00492,D00494,D00545,D00601,D00603,D00604,D00605,D00615,D00845,D00857,D00858,M00171,M00210,T00002,T00010,T00011,T00015,T00227,T00247,T00408,T00414,D00827,D00262,D00274,D00275,M00035,T00109 |
Enzyme Name | UniProtKB | KEGG |
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| Q2RSB2 | Q2RSB3 | Q2RSB4 |
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Protein name | NAD(P) transhydrogenase subunit alpha part 1 | NAD(P) transhydrogenase subunit alpha part 2 | NAD(P) transhydrogenase subunit beta | NAD(P)+ transhydrogenase (AB-specific)pyridine nucleotide transhydrogenasetranshydrogenaseNAD(P)+ transhydrogenasenicotinamide adenine dinucleotide (phosphate) transhydrogenaseNAD+ transhydrogenaseNADH transhydrogenasenicotinamide nucleotide transhydrogenaseNADPH-NAD+ transhydrogenasepyridine nucleotide transferaseNADPH-NAD+ oxidoreductaseNADH-NADP+-transhydrogenaseNADPH:NAD+ transhydrogenaseH+-Thaseenergy-linked transhydrogenaseNAD(P)+ transhydrogenase (AB-specific) |
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Synonyms | EC 1.6.1.2Nicotinamide nucleotide transhydrogenase subunit alpha 1Pyridine nucleotide transhydrogenase subunit alpha 1Proton-translocating transhydrogenase component 1dI | EC 1.6.1.2Nicotinamide nucleotide transhydrogenase subunit alpha 2Pyridine nucleotide transhydrogenase subunit alpha 2Proton-translocating transhydrogenase component 2dII | EC 1.6.1.2Nicotinamide nucleotide transhydrogenase subunit betaPyridine nucleotide transhydrogenase subunit betaProton-translocating transhydrogenase NADP(H)-binding componentdIII |
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RefSeq | YP_427270.1 (Protein) NC_007643.1 (DNA/RNA sequence)
| YP_427269.1 (Protein) NC_007643.1 (DNA/RNA sequence)
| YP_427268.1 (Protein) NC_007643.1 (DNA/RNA sequence)
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Pfam | PF01262 (AlaDh_PNT_C) PF05222 (AlaDh_PNT_N) [Graphical view]
| PF12769 (DUF3814) [Graphical view]
| PF02233 (PNTB) [Graphical view]
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KEGG pathways | MAP code | Pathways |
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MAP00760 | Nicotinate and nicotinamide metabolism |
UniProtKB:Accession Number | Q2RSB2 | Q2RSB3 | Q2RSB4 |
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Entry name | PNTAA_RHORT | PNTAB_RHORT | PNTB_RHORT |
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Activity | NADPH + NAD(+) = NADP(+) + NADH. | NADPH + NAD(+) = NADP(+) + NADH. | NADPH + NAD(+) = NADP(+) + NADH. |
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Subunit | Complex of an alpha and a beta chain, in Rhodospirillum, the alpha chain seems to be made of two subunits (By similarity). | Complex of an alpha and a beta chain, in Rhodospirillum, the alpha chain seems to be made of two subunits (By similarity). | Complex of an alpha and a beta chain, in Rhodospirillum, the alpha chain seems to be made of two subunits (By similarity). |
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Subcellular location |
| Cell inner membrane, Multi-pass membrane protein (By similarity). | Cell inner membrane, Multi-pass membrane protein (By similarity). |
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Cofactor |
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references | [1] |
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PubMed ID | 8011636 |
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Journal | Biochemistry |
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Year | 1994 |
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Volume | 33 |
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Pages | 7691-700 |
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Authors | Holmberg E, Olausson T, Hultman T, Rydstrom J, Ahmad S, Glavas NA, Bragg PD |
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Title | Prediction and site-specific mutagenesis of residues in transmembrane alpha-helices of proton-pumping nicotinamide nucleotide transhydrogenases from Escherichia coli and bovine heart mitochondria. |
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[2] |
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PubMed ID | 9804876 |
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Journal | Biochim Biophys Acta |
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Year | 1998 |
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Volume | 1409 |
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Pages | 25-38 |
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Authors | Gupta S, Quirk PG, Venning JD, Slade J, Bizouarn T, Grimley RL, Cotton NP, Jackson JB |
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Title | Mutation of amino acid residues in the mobile loop region of the NAD(H)-binding domain of proton-translocating transhydrogenase. |
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[3] |
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PubMed ID | 10393090 |
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Journal | Biochem J |
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Year | 1999 |
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Volume | 341 |
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Pages | 329-37 |
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Authors | Venning JD, Jackson JB |
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Title | A shift in the equilibrium constant at the catalytic site of proton-translocating transhydrogenase: significance for a 'binding-change' mechanism. |
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[4] |
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PubMed ID | 10514549 |
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Journal | Biochim Biophys Acta |
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Year | 1999 |
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Volume | 1413 |
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Pages | 81-91 |
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Authors | Peake SJ, Venning JD, Cotton NP, Jackson JB |
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Title | Evidence for the stabilization of NADPH relative to NADP(+) on the dIII components of proton-translocating transhydrogenases from Homo sapiens and from Rhodospirillum rubrum by measurement of tryptophan fluorescence. |
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[5] |
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PubMed ID | 10216162 |
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Journal | Biochim Biophys Acta |
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Year | 1999 |
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Volume | 1411 |
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Pages | 159-69 |
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Authors | Peake SJ, Venning JD, Jackson JB |
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Title | A catalytically active complex formed from the recombinant dI protein of Rhodospirillum rubrum transhydrogenase, and the recombinant dIII protein of the human enzyme. |
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[6] |
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PubMed ID | 10611473 |
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Journal | FEBS Lett |
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Year | 1999 |
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Volume | 464 |
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Pages | 1-8 |
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Authors | Jackson JB, Peake SJ, White SA |
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Title | Structure and mechanism of proton-translocating transhydrogenase. |
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[7] |
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PubMed ID | 10587945 |
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Journal | Microb Comp Genomics |
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Year | 1999 |
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Volume | 4 |
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Pages | 173-86 |
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Authors | Studley WK, Yamaguchi M, Hatefi Y, Saier MH Jr |
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Title | Phylogenetic analyses of proton-translocating transhydrogenases. |
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[8] |
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Comments | NMR Structure |
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PubMed ID | 11004437 |
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Journal | Biochim Biophys Acta |
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Year | 2000 |
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Volume | 1459 |
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Pages | 248-57 |
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Authors | Jeeves M, Smith KJ, Quirk PG, Cotton NP, Jackson JB |
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Title | Solution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from Rhodospirillum rubrum. |
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Related PDB | 1e3t |
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[9] |
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PubMed ID | 10747934 |
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Journal | J Biol Chem |
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Year | 2000 |
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Volume | 275 |
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Pages | 19490-7 |
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Authors | Venning JD, Peake SJ, Quirk PG, Jackson JB |
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Title | Stopped-flow reaction kinetics of recombinant components of proton-translocating transhydrogenase with physiological nucleotides. |
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[10] |
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PubMed ID | 10997900 |
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Journal | Structure Fold Des |
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Year | 2000 |
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Volume | 8 |
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Pages | 809-15 |
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Authors | Buckley PA, Baz Jackson J, Schneider T, White SA, Rice DW, Baker PJ |
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Title | Protein-protein recognition, hydride transfer and proton pumping in the transhydrogenase complex. |
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Related PDB | 1f8g |
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[11] |
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PubMed ID | 11231296 |
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Journal | Eur J Biochem |
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Year | 2001 |
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Volume | 268 |
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Pages | 1430-8 |
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Authors | Rodrigues DJ, Venning JD, Quirk PG, Jackson JB |
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Title | A change in ionization of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase regulates both hydride transfer and nucleotide release. |
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[12] |
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PubMed ID | 11577115 |
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Journal | J Biol Chem |
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Year | 2001 |
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Volume | 276 |
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Pages | 44757-61 |
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Authors | Pinheiro TJ, Venning JD, Jackson JB |
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Title | Fast hydride transfer in proton-translocating transhydrogenase revealed in a rapid mixing continuous flow device. |
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[13] |
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PubMed ID | 11399770 |
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Journal | J Biol Chem |
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Year | 2001 |
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Volume | 276 |
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Pages | 30678-85 |
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Authors | Venning JD, Rodrigues DJ, Weston CJ, Cotton NP, Quirk PG, Errington N, Finet S, White SA, Jackson JB |
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Title | The heterotrimer of the membrane-peripheral components of transhydrogenase and the alternating-site mechanism of proton translocation. |
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[14] |
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PubMed ID | 11250201 |
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Journal | Structure (Camb) |
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Year | 2001 |
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Volume | 9 |
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Pages | 165-76 |
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Authors | Cotton NP, White SA, Peake SJ, McSweeney S, Jackson JB |
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Title | The crystal structure of an asymmetric complex of the two nucleotide binding components of proton-translocating transhydrogenase. |
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Related PDB | 1hzz |
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[15] |
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PubMed ID | 12206884 |
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Journal | Biochim Biophys Acta |
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Year | 2002 |
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Volume | 1555 |
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Pages | 8-13 |
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Authors | Rodrigues DJ, Jackson JB |
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Title | A conformational change in the isolated NADP(H)-binding component (dIII) of transhydrogenase induced by low pH: a reflection of events during proton translocation by the complete enzyme? |
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[16] |
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PubMed ID | 12379117 |
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Journal | Biochemistry |
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Year | 2002 |
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Volume | 41 |
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Pages | 12745-54 |
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Authors | Prasad GS, Wahlberg M, Sridhar V, Sundaresan V, Yamaguchi M, Hatefi Y, Stout CD |
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Title | Crystal structures of transhydrogenase domain I with and without bound NADH. |
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Related PDB | 1l7d,1l7e |
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[17] |
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PubMed ID | 14567675 |
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Journal | Biochemistry |
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Year | 2003 |
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Volume | 42 |
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Pages | 12143-53 |
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Authors | Sundaresan V, Yamaguchi M, Chartron J, Stout CD |
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Title | Conformational change in the NADP(H) binding domain of transhydrogenase defines four states. |
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Related PDB | 1pno,1pnq |
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[18] |
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PubMed ID | 12564924 |
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Journal | Biochemistry |
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Year | 2003 |
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Volume | 42 |
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Pages | 1217-26 |
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Authors | van Boxel GI, Quirk PG, Cotton NP, White SA, Jackson JB |
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Title | Glutamine 132 in the NAD(H)-binding component of proton-translocating transhydrogenase tethers the nucleotides before hydride transfer. |
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Related PDB | 1nm5 |
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[19] |
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PubMed ID | 12765762 |
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Journal | Biochim Biophys Acta |
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Year | 2003 |
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Volume | 1604 |
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Pages | 55-9 |
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Authors | Pedersen A, Karlsson J, Althage M, Rydstrom J |
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Title | Properties of the apo-form of the NADP(H)-binding domain III of proton-pumping Escherichia coli transhydrogenase: implications for the reaction mechanism of the intact enzyme. |
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[20] |
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PubMed ID | 12972415 |
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Journal | J Biol Chem |
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Year | 2003 |
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Volume | 278 |
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Pages | 47578-84 |
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Authors | Broos J, Gabellieri E, van Boxel GI, Jackson JB, Strambini GB |
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Title | Tryptophan phosphorescence spectroscopy reveals that a domain in the NAD(H)-binding component (dI) of transhydrogenase from Rhodospirillum rubrum has an extremely rigid and conformationally homogeneous protein core. |
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[21] |
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Comments | X-ray crystallography |
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PubMed ID | 12791694 |
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Journal | J Biol Chem |
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Year | 2003 |
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Volume | 278 |
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Pages | 33208-16 |
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Authors | Singh A, Venning JD, Quirk PG, van Boxel GI, Rodrigues DJ, White SA, Jackson JB |
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Title | Interactions between transhydrogenase and thio-nicotinamide Analogues of NAD(H) and NADP(H) underline the importance of nucleotide conformational changes in coupling to proton translocation. |
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Related PDB | 1pt9,1ptj |
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[22] |
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PubMed ID | 15323555 |
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Journal | Biochemistry |
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Year | 2004 |
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Volume | 43 |
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Pages | 10952-64 |
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Authors | Mather OC, Singh A, van Boxel GI, White SA, Jackson JB |
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Title | Active-site conformational changes associated with hydride transfer in proton-translocating transhydrogenase. |
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Related PDB | 1u28,1u2d,1u2g,1u31 |
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comments | This enzyme is composed of two alpha chains and a beta chain. Alpha-1 chain is homologous to the domain I (N-terminal matrix domain) of NAD(P)+ transhydrogenase from mammalian mitochondrial membrane (Swissprot P11024, Q13423; M00162 of EzCatDB), whilst beta chain is thomologous to the domain III (C-terminal matrix domain) of the counterpart enzyme. Although the tertiary structures of these chains have been solved, the structure of the remainder, alpha-2 subunit, which is a membrane-bound protein, has not been determined yet.
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created | updated |
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2004-12-17 | 2009-02-26 |
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