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DB code | M00186 |
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CATH domain | Domain 1 | 3.20.20.140 : TIM Barrel |  | Catalytic domain |
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Domain 2 | -.-.-.- |  |
| Domain 3 | -.-.-.- |  | Catalytic domain | Domain 4 | 2.40.50.140 : OB fold (Dihydrolipoamide Acetyltransferase, E2P) |  |
| Domain 5 | -.-.-.- |  |
| Domain 6 | 3.30.420.10 : Nucleotidyltransferase; domain 5 |  | Catalytic domain | Domain 7 | -.-.-.- |  |
| Domain 8 | 1.10.1110.10 : DNA Polymerase III; Chain A |  |
| Domain 9 | 3.40.50.300 : Rossmann fold |  | Catalytic domain | Domain 10 | 1.20.272.10 : Zinc Finger, Delta Prime; domain 3 |  |
| Domain 11 | 1.10.8.60 : Helicase, Ruva Protein; domain 3 |  |
| Domain 12 | -.-.-.- |  |
| Domain 13 | -.-.-.- |  |
| Domain 14 | 3.40.50.300 : Rossmann fold |  |
| Domain 15 | 1.20.272.20 : Zinc Finger, Delta Prime; domain 3 |  |
| Domain 16 | 1.10.8.60 : Helicase, Ruva Protein; domain 3 |  |
| Domain 17 | 3.40.50.300 : Rossmann fold |  |
| Domain 18 | 1.10.8.10 : Helicase, Ruva Protein; domain 3 |  |
| Domain 19 | 1.20.272.10 : Zinc Finger, Delta Prime; domain 3 |  |
| Domain 20 | 3.40.50.10220 : Rossmann fold |  |
| Domain 21 | 3.40.50.10110 : Rossmann fold |  |
| Domain 22 | 3.10.150.10 : DNA Polymerase III; Chain A, domain 2 |  |
| Domain 23 | 3.10.150.10 : DNA Polymerase III; Chain A, domain 2 |  |
| Domain 24 | 3.10.150.10 : DNA Polymerase III; Chain A, domain 2 |  |
| E.C. | 2.7.7.7,3.1.11.1,3.6.1.3 |
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CSA | 1j53 |
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CATH domain | Related DB codes (homologues) |
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2.40.50.140 : OB fold (Dihydrolipoamide Acetyltransferase, E2P) | M00220,T00050,D00291,D00294,T00254 | 3.20.20.140 : TIM Barrel | S00231,S00232,D00673,D00675,D00801,D00873,M00030,M00225,M00226 | 3.30.420.10 : Nucleotidyltransferase; domain 5 | M00206,T00252,M00019,M00020,M00055,M00135,M00146,M00166,M00173,M00175 | 3.40.50.300 : Rossmann fold | S00527,S00547,S00548,S00550,S00554,S00555,S00671,S00672,S00676,S00680,S00682,S00913,S00914,S00301,S00302,S00303,S00304,S00307,S00308,S00305,S00306,S00309,S00310,S00311,M00114,M00199,D00129,D00130,D00540 |
UniProtKB:Accession Number | P10443 | P03007 | P0ABS8 | P06710 | P28630 | P28631 | P28632 | P28905 | P0A988 |
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Entry name | DPO3A_ECOLI | DPO3E_ECOLI | HOLE_ECOLI | DPO3X_ECOLI | HOLA_ECOLI | HOLB_ECOLI | HOLD_ECOLI | HOLC_ECOLI | DPO3B_ECOLI |
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Activity | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). |
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Subunit | The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4]. | The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4]. | The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4]. | The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4]. | The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4]. | The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4]. | The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4]. | The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4]. | The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3''-5'' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta'', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta'', psi,chi- beta[4]. The beta chain is a homodimer when not associated with the other components. |
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Subcellular location | Cytoplasm. |
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| Cytoplasm. |
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Cofactor |
| Binds 2 divalent metal cations. Magnesium or manganese. |
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Compound table: links to PDB-related databases & PoSSuM |
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| Cofactors | Substrates | Products |
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KEGG-id | C02148 | C00677 | C00039 | C00039 | C00001 | C00002 | C00013 | C00039 | C00039 | C01150 | C00008 | C00009 |
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E.C. | 2.7.7.7,3.1.11.1 | 2.7.7.7 | 2.7.7.7 | 3.1.11.1 | 3.1.11.1,3.6.1.3 | 3.6.1.3 | 2.7.7.7 | 2.7.7.7 | 3.1.11.1 | 3.1.11.1 | 3.6.1.3 | 3.6.1.3 |
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Compound | Divalent metal | Deoxynucleoside triphosphate | DNA(n) | DNA(n+1) | H2O | ATP | Pyrophosphate | DNA(n+1) | DNA(n) | 5'-Phosphomononucleotides | ADP | Orthophosphate |
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Type | divalent metal (Ca2+, Mg2+) | nucleotide | nucleic acids | nucleic acids | H2O | amine group,nucleotide | phosphate group/phosphate ion | nucleic acids | nucleic acids | nucleotide | amine group,nucleotide | phosphate group/phosphate ion |
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ChEBI |
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| 15377
| 15422
| 29888
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| 16761
| 26078
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PubChem |
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| 22247451 962
| 5957
| 1023 21961011
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| 6022
| 1004 22486802
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| | | | | | | | | | | | | | | | | | | |
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1j53A |  |  |  |  |  |  |  | Bound:2x_MN | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Analogue:TMP 2100 | Bound:TMP 2000 | Unbound | Unbound |
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1j54A |  |  |  |  |  |  |  | Bound:2x_MN | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Bound:TMP 2000 | Unbound | Unbound |
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1du2A |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2ae9A |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1jr3A01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Analogue:SO4 |
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1jr3B01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Analogue:SO4 |
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1jr3C01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Analogue:SO4 |
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1xxhB01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Analogue:ATG | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Bound:PO4 |
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1xxhD01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Analogue:ATG | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhG01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Analogue:ATG | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhH01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Bound:PO4 |
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1xxhI01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Analogue:ATG | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiB01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Bound:ADP | Unbound |
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1xxiC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Bound:PO4 |
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1xxiD01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Bound:ADP | Unbound |
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1xxiG01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiH01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiI01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Bound:ADP | Unbound |
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1njfA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Analogue:ATG | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1njfB01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Analogue:ATG | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1njfC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Bound:ADP | Unbound |
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1njfD01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Analogue:ATG | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1njgA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Analogue:SO4 |
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1njgB01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Analogue:SO4 |
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1jr3A02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1jr3B02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1jr3C02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhB02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhD02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhG02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhH02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhI02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiB02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiD02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiG02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiH02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiI02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1jr3A03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1jr3B03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1jr3C03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhB03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhC03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhD03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhG03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhH03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhI03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiB03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiC03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiD03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiG03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiH03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiI03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1njfA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1njfB02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1njfC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1njfD02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1njgA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1njgB02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1jr3D01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhF01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiF01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1jqjC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1jqjD01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1jqlB |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1jr3D02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxhF02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1xxiF02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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1jqjC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jqjD02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jr3D03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxhA03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxhF03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxiA03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxiF03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jqjC03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jqjD03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1a5tA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jr3E01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxhE01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxhJ01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxiE01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxiJ01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1a5tA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jr3E02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxhE02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxhJ02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxiE02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxiJ02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1a5tA03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jr3E03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxhE03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxhJ03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxiE03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1xxiJ03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1em8B |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1em8D |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1em8A |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1em8C |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jqjA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jqjB01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jqlA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1mmiA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1mmiB01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1ok7A01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1ok7B01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1unnA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1unnB01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
2polA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
2polB01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jqjA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jqjB02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jqlA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1mmiA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1mmiB02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1ok7A02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1ok7B02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1unnA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1unnB02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
2polA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
2polB02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jqjA03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jqjB03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1jqlA03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1mmiA03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1mmiB03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1ok7A03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1ok7B03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1unnA03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1unnB03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
2polA03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
2polB03 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
---|
[34] | Fig.5, p.540-543 |
|
references | [1] |
---|
PubMed ID | 7118945 |
---|
Journal | J Biol Chem |
---|
Year | 1982 |
---|
Volume | 257 |
---|
Pages | 12310-5 |
---|
Authors | Johanson KO, McHenry CS |
---|
Title | The beta subunit of the DNA polymerase III holoenzyme becomes inaccessible to antibody after formation of an initiation complex with primed DNA. |
---|
[2] |
---|
PubMed ID | 3037519 |
---|
Journal | Proc Natl Acad Sci U S A |
---|
Year | 1987 |
---|
Volume | 84 |
---|
Pages | 4389-92 |
---|
Authors | Maki H, Kornberg A |
---|
Title | Proofreading by DNA polymerase III of Escherichia coli depends on cooperative interaction of the polymerase and exonuclease subunits. |
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[3] |
---|
PubMed ID | 3283128 |
---|
Journal | J Biol Chem |
---|
Year | 1988 |
---|
Volume | 263 |
---|
Pages | 6570-8 |
---|
Authors | Maki H, Maki S, Kornberg A |
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Title | DNA Polymerase III holoenzyme of Escherichia coli. IV. The holoenzyme is an asymmetric dimer with twin active sites. |
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[4] |
---|
PubMed ID | 2243096 |
---|
Journal | J Biol Chem |
---|
Year | 1990 |
---|
Volume | 265 |
---|
Pages | 20356-63 |
---|
Authors | Griep MA, McHenry CS |
---|
Title | Dissociation of the DNA polymerase III holoenzyme beta 2 subunits is accompanied by conformational change at distal cysteines 333. |
---|
[5] |
---|
PubMed ID | 1918028 |
---|
Journal | J Biol Chem |
---|
Year | 1991 |
---|
Volume | 266 |
---|
Pages | 19127-30 |
---|
Authors | McHenry CS |
---|
Title | DNA polymerase III holoenzyme. Components, structure, and mechanism of a true replicative complex. |
---|
[6] |
---|
Comments | REVIEW. |
---|
Medline ID | 92246902 |
---|
PubMed ID | 1575709 |
---|
Journal | Bioessays |
---|
Year | 1992 |
---|
Volume | 14 |
---|
Pages | 105-11 |
---|
Authors | O'Donnell M |
---|
Title | Accessory protein function in the DNA polymerase III holoenzyme from E. coli. |
---|
Related UniProtKB | P28631 |
---|
[7] |
---|
PubMed ID | 1740452 |
---|
Journal | J Biol Chem |
---|
Year | 1992 |
---|
Volume | 267 |
---|
Pages | 4045-53 |
---|
Authors | Zechner EL, Wu CA, Marians KJ |
---|
Title | Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. II. Frequency of primer synthesis and efficiency of primer utilization control Okazaki fragment size. |
---|
[8] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) |
---|
Medline ID | 92257585 |
---|
PubMed ID | 1349852 |
---|
Journal | Cell |
---|
Year | 1992 |
---|
Volume | 69 |
---|
Pages | 425-37 |
---|
Authors | Kong XP, Onrust R, O'Donnell M, Kuriyan J |
---|
Title | Three-dimensional structure of the beta subunit of E. coli DNA polymerase III holoenzyme: a sliding DNA clamp. |
---|
Related PDB | 2pol |
---|
Related UniProtKB | P00583 |
---|
[9] |
---|
Comments | CHARACTERIZATION. |
---|
Medline ID | 93280137 |
---|
PubMed ID | 8505304 |
---|
Journal | J Biol Chem |
---|
Year | 1993 |
---|
Volume | 268 |
---|
Pages | 11766-72 |
---|
Authors | Onrust R, O'Donnell M |
---|
Title | DNA polymerase III accessory proteins. II. Characterization of delta and delta'. |
---|
Related UniProtKB | P28631 |
---|
[10] |
---|
PubMed ID | 7903401 |
---|
Journal | J Mol Biol |
---|
Year | 1993 |
---|
Volume | 234 |
---|
Pages | 915-25 |
---|
Authors | Kuriyan J, O'Donnell M |
---|
Title | Sliding clamps of DNA polymerases. |
---|
[11] |
---|
PubMed ID | 8505305 |
---|
Journal | J Biol Chem |
---|
Year | 1993 |
---|
Volume | 268 |
---|
Pages | 11779-84 |
---|
Authors | Xiao H, Dong Z, O'Donnell M |
---|
Title | DNA polymerase III accessory proteins. IV. Characterization of chi and psi. |
---|
[12] |
---|
PubMed ID | 8001157 |
---|
Journal | Cell |
---|
Year | 1994 |
---|
Volume | 79 |
---|
Pages | 1233-43 |
---|
Authors | Krishna TS, Kong XP, Gary S, Burgers PM, Kuriyan J |
---|
Title | Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA. |
---|
[13] |
---|
PubMed ID | 8300534 |
---|
Journal | J Bacteriol |
---|
Year | 1994 |
---|
Volume | 176 |
---|
Pages | 815-21 |
---|
Authors | Slater SC, Lifsics MR, O'Donnell M, Maurer R |
---|
Title | holE, the gene coding for the theta subunit of DNA polymerase III of Escherichia coli: characterization of a holE mutant and comparison with a dnaQ (epsilon-subunit) mutant. |
---|
[14] |
---|
PubMed ID | 7768937 |
---|
Journal | J Biol Chem |
---|
Year | 1995 |
---|
Volume | 270 |
---|
Pages | 13358-65 |
---|
Authors | Naktinis V, Onrust R, Fang L, O'Donnell M |
---|
Title | Assembly of a chromosomal replication machine: two DNA polymerases, a clamp loader, and sliding clamps in one holoenzyme particle. II. Intermediate complex between the clamp loader and its clamp. |
---|
[15] |
---|
PubMed ID | 7768938 |
---|
Journal | J Biol Chem |
---|
Year | 1995 |
---|
Volume | 270 |
---|
Pages | 13366-77 |
---|
Authors | Onrust R, Finkelstein J, Turner J, Naktinis V, O'Donnell M |
---|
Title | Assembly of a chromosomal replication machine: two DNA polymerases, a clamp loader, and sliding clamps in one holoenzyme particle. III. Interface between two polymerases and the clamp loader. |
---|
[16] |
---|
PubMed ID | 7768939 |
---|
Journal | J Biol Chem |
---|
Year | 1995 |
---|
Volume | 270 |
---|
Pages | 13378-83 |
---|
Authors | Xiao H, Naktinis V, O'Donnell M |
---|
Title | Assembly of a chromosomal replication machine: two DNA polymerases, a clamp loader, and sliding clamps in one holoenzyme particle. IV. ATP-binding site mutants identify the clamp loader. |
---|
[17] |
---|
PubMed ID | 7478986 |
---|
Journal | Nucleic Acids Res |
---|
Year | 1995 |
---|
Volume | 23 |
---|
Pages | 3613-20 |
---|
Authors | Kelman Z, O'Donnell M |
---|
Title | Structural and functional similarities of prokaryotic and eukaryotic DNA polymerase sliding clamps. |
---|
[18] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS). |
---|
Medline ID | 98028572 |
---|
PubMed ID | 9363942 |
---|
Journal | Cell |
---|
Year | 1997 |
---|
Volume | 91 |
---|
Pages | 335-45 |
---|
Authors | Guenther B, Onrust R, Sali A, O'Donnell M, Kuriyan J |
---|
Title | Crystal structure of the delta' subunit of the clamp-loader complex of E. coli DNA polymerase III. |
---|
Related PDB | 1a5t |
---|
Related UniProtKB | P28631 |
---|
[19] |
---|
PubMed ID | 9346941 |
---|
Journal | J Biol Chem |
---|
Year | 1997 |
---|
Volume | 272 |
---|
Pages | 27919-30 |
---|
Authors | Bloom LB, Chen X, Fygenson DK, Turner J, O'Donnell M, Goodman MF |
---|
Title | Fidelity of Escherichia coli DNA polymerase III holoenzyme. The effects of beta, gamma complex processivity proteins and epsilon proofreading exonuclease on nucleotide misincorporation efficiencies. |
---|
[20] |
---|
PubMed ID | 9753470 |
---|
Journal | Biochemistry |
---|
Year | 1998 |
---|
Volume | 37 |
---|
Pages | 13807-15 |
---|
Authors | Terashima I, Suzuki N, Dasaradhi L, Tan CK, Downey KM, Shibutani S |
---|
Title | Translesional synthesis on DNA templates containing an estrogen quinone-derived adduct: N2-(2-hydroxyestron-6-yl)-2'-deoxyguanosine and N6-(2-hydroxyestron-6-yl)-2'-deoxyadenosine. |
---|
[21] |
---|
PubMed ID | 9685491 |
---|
Journal | Nucleic Acids Res |
---|
Year | 1998 |
---|
Volume | 26 |
---|
Pages | 3746-52 |
---|
Authors | Aravind L, Koonin EV |
---|
Title | Phosphoesterase domains associated with DNA polymerases of diverse origins. |
---|
[22] |
---|
PubMed ID | 11101526 |
---|
Journal | EMBO J |
---|
Year | 2000 |
---|
Volume | 19 |
---|
Pages | 6536-45 |
---|
Authors | Pritchard AE, Dallmann HG, Glover BP, McHenry CS |
---|
Title | A novel assembly mechanism for the DNA polymerase III holoenzyme DnaX complex: association of deltadelta' with DnaX(4) forms DnaX(3)deltadelta'. |
---|
[23] |
---|
Comments | X-ray crystallography |
---|
PubMed ID | 10794414 |
---|
Journal | Protein Sci |
---|
Year | 2000 |
---|
Volume | 9 |
---|
Pages | 721-33 |
---|
Authors | Keniry MA, Berthon HA, Yang JY, Miles CS, Dixon NE |
---|
Title | NMR solution structure of the theta subunit of DNA polymerase III from Escherichia coli. |
---|
Related PDB | 1du2 |
---|
[24] |
---|
Comments | X-ray crystallography |
---|
PubMed ID | 11525729 |
---|
Journal | Cell |
---|
Year | 2001 |
---|
Volume | 106 |
---|
Pages | 429-41 |
---|
Authors | Jeruzalmi D, O'Donnell M, Kuriyan J |
---|
Title | Crystal structure of the processivity clamp loader gamma (gamma) complex of E. coli DNA polymerase III. |
---|
Related PDB | 1jr3 |
---|
[25] |
---|
PubMed ID | 11518714 |
---|
Journal | J Biol Chem |
---|
Year | 2001 |
---|
Volume | 276 |
---|
Pages | 40668-79 |
---|
Authors | Song MS, Dallmann HG, McHenry CS |
---|
Title | Carboxyl-terminal domain III of the delta' subunit of the DNA polymerase III holoenzyme binds delta. |
---|
[26] |
---|
PubMed ID | 11606586 |
---|
Journal | J Biol Chem |
---|
Year | 2001 |
---|
Volume | 276 |
---|
Pages | 48709-15 |
---|
Authors | Song MS, McHenry CS |
---|
Title | Carboxyl-terminal domain III of the delta' subunit of DNA polymerase III holoenzyme binds DnaX and supports cooperative DnaX complex assembly. |
---|
[27] |
---|
PubMed ID | 11572866 |
---|
Journal | J Biol Chem |
---|
Year | 2001 |
---|
Volume | 276 |
---|
Pages | 47185-94 |
---|
Authors | Leu FP, O'Donnell M |
---|
Title | Interplay of clamp loader subunits in opening the beta sliding clamp of Escherichia coli DNA polymerase III holoenzyme. |
---|
[28] |
---|
PubMed ID | 11279099 |
---|
Journal | J Biol Chem |
---|
Year | 2001 |
---|
Volume | 276 |
---|
Pages | 19182-9 |
---|
Authors | Stewart J, Hingorani MM, Kelman Z, O'Donnell M |
---|
Title | Mechanism of beta clamp opening by the delta subunit of Escherichia coli DNA polymerase III holoenzyme. |
---|
[29] |
---|
PubMed ID | 11719243 |
---|
Journal | Curr Biol |
---|
Year | 2001 |
---|
Volume | 11 |
---|
Pages | R935-46 |
---|
Authors | O'Donnell M, Jeruzalmi D, Kuriyan J |
---|
Title | Clamp loader structure predicts the architecture of DNA polymerase III holoenzyme and RFC. |
---|
[30] |
---|
PubMed ID | 11525728 |
---|
Journal | Cell |
---|
Year | 2001 |
---|
Volume | 106 |
---|
Pages | 417-28 |
---|
Authors | Jeruzalmi D, Yurieva O, Zhao Y, Young M, Stewart J, Hingorani M, O'Donnell M, Kuriyan J |
---|
Title | Mechanism of processivity clamp opening by the delta subunit wrench of the clamp loader complex of E. coli DNA polymerase III. |
---|
Related PDB | 1jqj,1jql |
---|
[31] |
---|
PubMed ID | 11859073 |
---|
Journal | J Biol Chem |
---|
Year | 2002 |
---|
Volume | 277 |
---|
Pages | 17334-48 |
---|
Authors | Bruck I, Yuzhakov A, Yurieva O, Jeruzalmi D, Skangalis M, Kuriyan J, O'Donnell M |
---|
Title | Analysis of a multicomponent thermostable DNA polymerase III replicase from an extreme thermophile. |
---|
[32] |
---|
PubMed ID | 11809766 |
---|
Journal | J Biol Chem |
---|
Year | 2002 |
---|
Volume | 277 |
---|
Pages | 13246-56 |
---|
Authors | Bullard JM, Pritchard AE, Song MS, Glover BP, Wieczorek A, Chen J, Janjic N, McHenry CS |
---|
Title | A three-domain structure for the delta subunit of the DNA polymerase III holoenzyme delta domain III binds delta' and assembles into the DnaX complex. |
---|
[33] |
---|
PubMed ID | 11772007 |
---|
Journal | Biochemistry |
---|
Year | 2002 |
---|
Volume | 41 |
---|
Pages | 94-110 |
---|
Authors | DeRose EF, Li D, Darden T, Harvey S, Perrino FW, Schaaper RM, London RE |
---|
Title | Model for the catalytic domain of the proofreading epsilon subunit of Escherichia coli DNA polymerase III based on NMR structural data. |
---|
[34] |
---|
PubMed ID | 11937058 |
---|
Journal | Structure |
---|
Year | 2002 |
---|
Volume | 10 |
---|
Pages | 535-46 |
---|
Authors | Hamdan S, Carr PD, Brown SE, Ollis DL, Dixon NE |
---|
Title | Structural basis for proofreading during replication of the Escherichia coli chromosome. |
---|
Related PDB | 1j53,1j54 |
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[35] |
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PubMed ID | 14592985 |
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Journal | EMBO J |
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Year | 2003 |
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Volume | 22 |
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Pages | 5883-92 |
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Authors | Bunting KA, Roe SM, Pearl LH |
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Title | Structural basis for recruitment of translesion DNA polymerase Pol IV/DinB to the beta-clamp. |
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Related PDB | 1unn |
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[36] |
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PubMed ID | 12832762 |
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Journal | Acta Crystallogr D Biol Crystallogr |
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Year | 2003 |
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Volume | 59 |
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Pages | 1192-9 |
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Authors | Oakley AJ, Prosselkov P, Wijffels G, Beck JL, Wilce MC, Dixon NE |
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Title | Flexibility revealed by the 1.85 A crystal structure of the beta sliding-clamp subunit of Escherichia coli DNA polymerase III. |
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Related PDB | 1mmi |
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[37] |
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PubMed ID | 12623013 |
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Journal | Structure |
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Year | 2003 |
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Volume | 11 |
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Pages | 253-63 |
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Authors | Podobnik M, Weitze TF, O'Donnell M, Kuriyan J |
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Title | Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit. |
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Related PDB | 1njf,1njg |
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[38] |
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PubMed ID | 14717711 |
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Journal | Eur J Biochem |
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Year | 2004 |
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Volume | 271 |
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Pages | 439-49 |
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Authors | Gulbis JM, Kazmirski SL, Finkelstein J, Kelman Z, O'Donnell M, Kuriyan J |
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Title | Crystal structure of the chi:psi sub-assembly of the Escherichia coli DNA polymerase clamp-loader complex. |
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Related PDB | 1em8 |
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[39] |
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PubMed ID | 14610068 |
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Journal | J Biol Chem |
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Year | 2004 |
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Volume | 279 |
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Pages | 4386-93 |
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Authors | Snyder AK, Williams CR, Johnson A, O'Donnell M, Bloom LB |
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Title | Mechanism of loading the Escherichia coli DNA polymerase III sliding clamp: II. Uncoupling the beta and DNA binding activities of the gamma complex. |
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[40] |
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PubMed ID | 15037068 |
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Journal | J Mol Biol |
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Year | 2004 |
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Volume | 336 |
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Pages | 1047-59 |
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Authors | Goedken ER, Levitus M, Johnson A, Bustamante C, O'Donnell M, Kuriyan J |
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Title | Fluorescence measurements on the E.coli DNA polymerase clamp loader: implications for conformational changes during ATP and clamp binding. |
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[41] |
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PubMed ID | 14729336 |
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Journal | J Mol Biol |
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Year | 2004 |
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Volume | 335 |
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Pages | 1187-97 |
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Authors | Burnouf DY, Olieric V, Wagner J, Fujii S, Reinbolt J, Fuchs RP, Dumas P |
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Title | Structural and biochemical analysis of sliding clamp/ligand interactions suggest a competition between replicative and translesion DNA polymerases. |
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Related PDB | 1ok7 |
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[42] |
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PubMed ID | 15556993 |
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Journal | Proc Natl Acad Sci U S A |
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Year | 2004 |
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Volume | 101 |
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Pages | 16750-5 |
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Authors | Kazmirski SL, Podobnik M, Weitze TF, O'Donnell M, Kuriyan J |
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Title | Structural analysis of the inactive state of the Escherichia coli DNA polymerase clamp-loader complex. |
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Related PDB | 1xxh,1xxi |
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[43] |
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PubMed ID | 16199579 |
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Journal | J Bacteriol |
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Year | 2005 |
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Volume | 187 |
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Pages | 7081-9 |
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Authors | Mueller GA, Kirby TW, DeRose EF, Li D, Schaaper RM, London RE |
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Title | Nuclear magnetic resonance solution structure of the Escherichia coli DNA polymerase III theta subunit. |
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Related PDB | 2ae9 |
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comments | This enzyme belongs to the DNA polymerase type-C family. This DNA enzyme is composed of 10 different types of subunits. According to the literature, these subunits are organized into 3 functionally essential subassemblies: (a) the pol III core (subunits alpha, epsilon and theta) (b) the clamp-loading complex (subunits delta, delta', psi and chi, and either or both of gamma and tau) (c) the beta sliding clamp processivity factor (subunit beta) The pol III core contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase (a) is tethered to the template via the sliding clamp processivity factor (c). The clamp-loading complex (b) assembles the beta processivity factor (c) onto the primer template and plays a central role in the organization and communication at the replication fork. The composition of the enzyme is as follows: (a) (alpha, epsilon, theta)[2] (b) (gamma/tau)[3]-(delta, delta', psi, chi)[2] (c) (beta[2])[2] The roles of the subunits are as follows: (a) (alpha, epsilon, theta)[2]; alpha subunit: A polymerase subunit. epsilon subunit: A proofreading subunit, with 3'-5' exonuclease activity. theta subunit: This subunit might maintain fidelity. (b) (gamma/tau)[3]-(delta, delta', psi, chi)[2]; gamma subunit: This subunit is a short vaiant of dnaX and interacts with delta subunit to transfer the beta subunit on the DNA. Moreover, this subunit has ATPase activity. tau subunit: This subunit is a long vaiant of dnaX, and also a scaffold to help in the dimerization of the core complex. delta subunit: This subunit interacts with gamma subunit to transfer the beta subunit on the DNA. delta' subunit: The function is unknown. chi subunit: The function is unknown. psi subunit: The function is unknown. (c) (beta[2])[2]; beta subunit: This subunit seems to be required for initiation of replication.
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created | updated |
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2005-03-30 | 2009-02-26 |
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