EzCatDB: M00207
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DB codeM00207
CATH domainDomain 13.-.-.-
Domain 22.40.-.-
Domain 3-.-.-.-
Domain 4-.-.-.-
Domain 5-.-.-.-
Domain 63.-.-.-
Domain 7-.-.-.-
Domain 8-.-.-.-
Domain 92.40.270.10 : Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain
Domain 102.40.50.150 : OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Domain 11-.-.-.-
Domain 123.90.-.-
Domain 133.90.-.-
Domain 143.30.1360.10 : Gyrase A; domain 2
Domain 152.170.-.-
Domain 163.30.70.20 : Alpha-Beta Plaits
Domain 173.90.940.20 : Eukaryotic RPB6 RNA polymerase subunit
Domain 183.90.940.10 : Eukaryotic RPB6 RNA polymerase subunit
Domain 193.30.1360.10 : Gyrase A; domain 2
Domain 202.20.25.10 : N-terminal domain of TfIIb
Domain 212.20.25.10 : N-terminal domain of TfIIb
Domain 221.10.10.60 : Arc Repressor Mutant, subunit A
E.C.2.7.7.6

CATH domainRelated DB codes (homologues)
2.20.25.10 : N-terminal domain of TfIIbM00034
2.40.270.10 : Dna-directed Rna Polymerase Ii 140kd Polypeptide; ChainM00208
2.40.50.150 : OB fold (Dihydrolipoamide Acetyltransferase, E2P)M00208
3.30.1360.10 : Gyrase A; domain 2M00208
3.30.70.20 : Alpha-Beta PlaitsM00012,M00042

Enzyme Name
UniProtKBKEGG

O27125O27126O27124O27123O26144O27122O26148O27372O27369O26147
Protein nameDNA-directed RNA polymerase subunit A''DNA-directed RNA polymerase subunit A''''DNA-directed RNA polymerase subunit B''DNA-directed RNA polymerase subunit B''''DNA-directed RNA polymerase subunit DDNA-directed RNA polymerase subunit HDNA-directed RNA polymerase subunit KDNA-directed RNA polymerase subunit LDNA-directed RNA polymerase subunit MDNA-directed RNA polymerase subunit NDNA-directed RNA polymerase
RNA polymerase
RNA nucleotidyltransferase (DNA-directed)
RNA polymerase I
RNA polymerase II
RNA polymerase III
RNA nucleotidyltransferase (DNA-directed)
C RNA formation factors
deoxyribonucleic acid-dependent ribonucleic acid polymerase
DNA-dependent ribonucleate nucleotidyltransferase
DNA-dependent RNA nucleotidyltransferase
DNA-dependent RNA polymerase
ribonucleate nucleotidyltransferase
ribonucleate polymerase
C ribonucleic acid formation factors
ribonucleic acid nucleotidyltransferase
ribonucleic acid polymerase
ribonucleic acid transcriptase
ribonucleic polymerase
ribonucleic transcriptase
C RNA formation factors
RNA nucleotidyltransferase
RNA transcriptase
transcriptase
SynonymsEC 2.7.7.6
EC 2.7.7.6
EC 2.7.7.6
EC 2.7.7.6
EC 2.7.7.6
EC 2.7.7.6
RPB5
EC 2.7.7.6
EC 2.7.7.6
EC 2.7.7.6
EC 2.7.7.6
RPB10
RefSeqNP_276181.1 (Protein)
NC_000916.1 (DNA/RNA sequence)
NP_276182.1 (Protein)
NC_000916.1 (DNA/RNA sequence)
NP_276180.1 (Protein)
NC_000916.1 (DNA/RNA sequence)
NP_276179.1 (Protein)
NC_000916.1 (DNA/RNA sequence)
NP_275181.1 (Protein)
NC_000916.1 (DNA/RNA sequence)
NP_276178.1 (Protein)
NC_000916.1 (DNA/RNA sequence)
NP_275185.1 (Protein)
NC_000916.1 (DNA/RNA sequence)
NP_276434.1 (Protein)
NC_000916.1 (DNA/RNA sequence)
NP_276431.1 (Protein)
NC_000916.1 (DNA/RNA sequence)
NP_275184.1 (Protein)
NC_000916.1 (DNA/RNA sequence)
PfamPF04997 (RNA_pol_Rpb1_1)
PF00623 (RNA_pol_Rpb1_2)
PF04983 (RNA_pol_Rpb1_3)
PF05000 (RNA_pol_Rpb1_4)
PF04998 (RNA_pol_Rpb1_5)
[Graphical view]
PF04998 (RNA_pol_Rpb1_5)
[Graphical view]
PF04566 (RNA_pol_Rpb2_4)
PF04567 (RNA_pol_Rpb2_5)
PF00562 (RNA_pol_Rpb2_6)
PF04560 (RNA_pol_Rpb2_7)
[Graphical view]
PF04563 (RNA_pol_Rpb2_1)
PF04561 (RNA_pol_Rpb2_2)
PF04565 (RNA_pol_Rpb2_3)
[Graphical view]
PF13237 (Fer4_10)
PF01000 (RNA_pol_A_bac)
PF01193 (RNA_pol_L)
[Graphical view]
PF01191 (RNA_pol_Rpb5_C)
[Graphical view]
PF01192 (RNA_pol_Rpb6)
[Graphical view]

PF02150 (RNA_POL_M_15KD)
PF01096 (TFIIS_C)
[Graphical view]
PF01194 (RNA_pol_N)
[Graphical view]

KEGG pathways
MAP codePathways
MAP00230Purine metabolism
MAP00240Pyrimidine metabolism

UniProtKB:Accession NumberO27125O27126O27124O27123O26144O27122O26148O27372O27369O26147
Entry nameRPOA1_METTHRPOA2_METTHRPOB1_METTHRPOB2_METTHRPOD_METTHRPOH_METTHRPOK_METTHRPOL_METTHRPOM_METTHRPON_METTH
ActivityNucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
SubunitM.thermoautotrophicum RNA polymerase is composed of four subunits: A'',A'''',B'' and B''''.This RNA polymerase is composed of four subunits: A'',A'''',B'' and B'''' (By similarity).M.thermoautotrophicum RNA polymerase is composed of four subunits: A'',A'''',B'' and B''''.M.thermoautotrophicum RNA polymerase is composed of four subunits: A'',A'''',B'' and B''''.





Subcellular location









CofactorZinc.
Zinc.Zinc.






Compound table: links to PDB-related databases & PoSSuM

SubstratesProducts
KEGG-idC00201C00046C00013C00046
CompoundNucleoside triphosphateRNA(n)PyrophosphateRNA(n+1)
Typenucleotidenucleic acidsphosphate group/phosphate ionnucleic acids
ChEBI

29888

PubChem

21961011
1023

            
1eikAUnboundUnboundUnboundUnbound
1ef4AUnboundUnboundUnboundUnbound

Active-site residues
pdb
        
1eikA
1ef4A


references
[1]
PubMed ID10841539
JournalProc Natl Acad Sci U S A
Year2000
Volume97
Pages6316-21
AuthorsMackereth CD, Arrowsmith CH, Edwards AM, McIntosh LP
TitleZinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum.
Related PDB1ef4
Related UniProtKBO26147
[2]
PubMed ID10841538
JournalProc Natl Acad Sci U S A
Year2000
Volume97
Pages6311-5
AuthorsYee A, Booth V, Dharamsi A, Engel A, Edwards AM, Arrowsmith CH
TitleSolution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum.
Related PDB1eik
Related UniProtKBO27122

comments
The same E.C. number (2.7.7.6) appears in M00104.
This enzyme seems to be composed of several subunits (A', A", B', B", D, H, K, L, M and N). However, the tertiary structures have been reported for the subunits, H and N, which are not catalytic subunits.
The subunit, A' (Swiss-prot;O27125), seems to have a catalytic domain. Although its tertiary structure has not been elucidated, the structure of its evolutionarily-related proteins have been reported (PDB;1i3qA, 1i50A).

createdupdated
2002-08-302009-02-26


Copyright: Nozomi Nagano, JST & CBRC-AIST
Funded by PRESTO/Japan Science and Technology Corporation (JST) (December 2001 - November 2004)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005 - September 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2012 - March 2013)
Supported by the commission for the Development of Artificial Gene Synthesis Technology for Creating Innovative Biomaterial from the Ministry of Economy, Trade and Industry (METI) (October 2012 - )
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