EzCatDB: M00317
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DB codeM00317
RLCP classification1.13.30000.10 : Hydrolysis
CATH domainDomain 13.40.50.410 : Rossmann fold
Domain 22.40.10.10 : Thrombin, subunit HCatalytic domain
Domain 32.40.10.10 : Thrombin, subunit HCatalytic domain
Domain 42.-.-.-
Domain 52.60.40.690 : Immunoglobulin-like
Domain 62.60.120.290 : Jelly Rolls
Domain 71.50.10.20 : Glycosyltransferase
Domain 82.60.40.690 : Immunoglobulin-like
Domain 92.40.50.120 : OB fold (Dihydrolipoamide Acetyltransferase, E2P)
E.C.3.4.21.47

CATH domainRelated DB codes (homologues)
2.40.10.10 : Thrombin, subunit HM00139,D00214,M00167,D00426,M00133,D00428,D00429,D00430,D00431,D00432,D00433,D00434,D00435,M00227,M00209,D00194,D00197,D00211,D00212,D00216,M00212,D00224,D00497,M00217,M00216,D00528,D00848,D00850,D00851,D00852,D00855,M00152,M00155,M00157,M00181,M00315,M00316,M00348,M00349,T00074,T00410,T00411
2.60.120.290 : Jelly RollsM00139,M00227,M00315,M00316,M00348

Enzyme Name
UniProtKBKEGG

P00751P01024Q91132
Protein nameComplement factor BComplement C3Cobra venom factorAlternative-complement-pathway C3/C5 convertase
Complement component C3/C5 convertase (alternative)
Proenzyme factor B
Properdin factor B
C3 proactivator
Glycine-rich beta-glycoprotein
Heat-labile factor
C3 convertase
C3b,Bb,CVF,Bb,C5 convertase
(C3b)n,Bb
Complement C 3(C 5) convertase (amplification)
Alternative complement pathway C3(C5) convertase
C5 convertase
CVF,Bb
(CVF)-dependent glycine-rich-beta-glucoprotein
Cobra venom factor-dependent C3 convertase
SynonymsEC 3.4.21.47
C3/C5 convertase
Glycine-rich beta glycoprotein
GBG
PBF2
Properdin factor B
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
CVF
CVFk
Complement C3 homolog
ContainsComplement factor B Ba fragment
Complement factor B Bb fragment
Complement C3 beta chain
Complement C3 alpha chain
C3a anaphylatoxin
Acylation stimulating protein
(ASP)
C3adesArg
Complement C3b alpha'' chain
Complement C3c alpha'' chain fragment 1
Complement C3dg fragment
Complement C3g fragment
Complement C3d fragment
Complement C3f fragment
Complement C3c alpha'' chain fragment 2
Cobra venom factor alpha chain
Cobra venom factor gamma chain
Cobra venom factor beta chain
RefSeqNP_001701.2 (Protein)
NM_001710.5 (DNA/RNA sequence)
NP_000055.2 (Protein)
NM_000064.2 (DNA/RNA sequence)

MEROPSS01.196 (Serine)


PfamPF00084 (Sushi)
PF00089 (Trypsin)
PF00092 (VWA)
[Graphical view]
PF00207 (A2M)
PF07678 (A2M_comp)
PF01835 (A2M_N)
PF07703 (A2M_N_2)
PF07677 (A2M_recep)
PF01821 (ANATO)
PF01759 (NTR)
PF10569 (Thiol-ester_cl)
[Graphical view]
PF00207 (A2M)
PF07678 (A2M_comp)
PF01835 (A2M_N)
PF07703 (A2M_N_2)
PF07677 (A2M_recep)
PF01821 (ANATO)
PF01759 (NTR)
PF10569 (Thiol-ester_cl)
[Graphical view]


UniProtKB:Accession NumberP00751P01024Q91132
Entry nameCFAB_HUMANCO3_HUMANCO3_NAJKA
ActivityCleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|-Xaa bond in complement component C5 alpha-chain to yield C5a and C5b.

SubunitMonomer.C3 precursor is first processed by the removal of 4 Arg residues, forming two chains, beta and alpha, linked by a disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylatoxin and generating C3b (beta chain + alpha' chain). During pregnancy, C3dg exists as a complex (probably a 2:2:2 heterohexamer) with AGT and the proform of PRG2. Interacts with CR2 and VSIG4.Heterotrimer of alpha, beta and gamma chains. disulfide-linked.
Subcellular locationSecreted.Secreted.Secreted.
Cofactor



Compound table: links to PDB-related databases & PoSSuM

CofactorsSubstratesProductsintermediates
KEGG-idC00305L00091L00092C00001L00093L00079L00094L00095I00087I00085I00086
CompoundMagnesiumComplement C3 alpha chainComplement C5 alpha chainH2OComplement component C3aComplement component C3bComplement component C5aComplement component C5bPeptidyl-tetrahedral intermediateAcyl-enzymetetrahedral intermediate
Typedivalent metal (Ca2+, Mg2+)peptide/proteinpeptide/proteinH2Opeptide/proteinpeptide/proteinpeptide/proteinpeptide/protein


ChEBI18420


15377







PubChem888


962
22247451







                   
1q0pA00Analogue:_MNUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1rrkA01Analogue:_COUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1rs0A01Bound:_MGUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1rtkA01Bound:_MGUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2ok5A04UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winI01Bound:_MGUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winJ01Bound:_MGUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winK01Bound:_MGUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winL01Bound:_MGUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbF04Bound:_MGUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbH04Bound:_MGUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjI04Analogue:_NIUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjJ04Analogue:_NIUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjK04Analogue:_NIUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjL04Analogue:_NIUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hrzD04Bound:_MGUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0D04Bound:_MGUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0I04Bound:_MGUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1rrkA02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1rs0A02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1rtkA02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dleA01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dleB01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2ok5A05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winI02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winJ02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winK02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winL02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbF05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbH05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjI05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjJ05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjK05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjL05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hrzD05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0D05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0I05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1rrkA03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1rs0A03UnboundUnboundUnbound UnboundUnboundUnboundUnboundTransition-state-analogue:DFPUnboundUnbound
1rtkA03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundIntermediate-analogue:GBSUnbound
1dleA02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
1dleB02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2ok5A06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winI03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winJ03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winK03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winL03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbF06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbH06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjI06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjJ06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjK06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjL06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hrzD06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0D06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0I06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winB01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winD01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winF01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winH01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbB01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbD01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjB01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjD01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjF01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjH01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hrzB01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0B01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0G01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winB02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winD02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winF02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winH02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbB02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbD02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjB02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjD02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjF02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjH02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hrzB02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0B02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0G02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winB03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winD03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winF03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winH03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbB03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbD03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjB03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjD03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjF03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjH03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hrzC01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0C01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0H01UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winB04UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winD04UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winF04UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winH04UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbB04UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbD04UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjB04UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjD04UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjF04UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjH04UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winB05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winD05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winF05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winH05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbB05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbD05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjB05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjD05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjF05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjH05UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hrzC02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0C02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0H02UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winB06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winD06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winF06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2winH06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbB06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwbD06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjB06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjD06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjF06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
2xwjH06UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hrzC03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0C03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound
3hs0H03UnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnboundUnbound

Active-site residues
resource
Literature [8] & Swiss-prot;P00751
pdbCatalytic residuesCofactor-binding residuesMain-chain involved in catalysis
           
1q0pA00 
SER 253;SER 255;THR 328(Magnesium binding)
 
1rrkA01 
SER 253;SER 255;THR 328(Magnesium binding)
 
1rs0A01 
SER 253;SER 255;THR 328(Magnesium binding)
 
1rtkA01 
SER 253;SER 255;THR 328(Magnesium binding)
 
2ok5A04 
SER 253;SER 255;THR 328(Magnesium binding)
 
2winI01 
SER 253;SER 255;THR 328(Magnesium binding)
 
2winJ01 
SER 253;SER 255;THR 328(Magnesium binding)
 
2winK01 
SER 253;SER 255;THR 328(Magnesium binding)
 
2winL01 
SER 253;SER 255;THR 328(Magnesium binding)
 
2xwbF04 
SER 253;SER 255;THR 328(Magnesium binding)
 
2xwbH04 
SER 253;SER 255;THR 328(Magnesium binding)
 
2xwjI04 
SER 253;SER 255;THR 328(Magnesium binding)
 
2xwjJ04 
SER 253;SER 255;THR 328(Magnesium binding)
 
2xwjK04 
SER 253;SER 255;THR 328(Magnesium binding)
 
2xwjL04 
SER 253;SER 255;THR 328(Magnesium binding)
 
3hrzD04 
SER 253;SER 255;THR 328(Magnesium binding)
 
3hs0D04 
SER 253;SER 255;THR 328(Magnesium binding)
 
3hs0I04 
SER 253;SER 255;THR 328(Magnesium binding)
 
1rrkA02HIS 501;ASP 551
 
 
1rs0A02HIS 501;ASP 551
 
 
1rtkA02HIS 501;ASP 551
 
 
1dleA01HIS  57;ASP 102
 
 
1dleB01HIS  57;ASP 102
 
 
2ok5A05HIS 501;ASP 551
 
 
2winI02HIS 501;ASP 551
 
 
2winJ02HIS 501;ASP 551
 
 
2winK02HIS 501;ASP 551
 
 
2winL02HIS 501;ASP 551
 
 
2xwbF05HIS 501;ASP 551
 
 
2xwbH05HIS 501;ASP 551
 
 
2xwjI05HIS 501;ASP 551
 
 
2xwjJ05HIS 501;ASP 551
 
 
2xwjK05HIS 501;ASP 551
 
 
2xwjL05HIS 501;ASP 551
 
 
3hrzD05HIS 501;ASP 551
 
 
3hs0D05HIS 501;ASP 551
 
 
3hs0I05HIS 501;ASP 551
 
 
1rrkA03SER 674
 
GLY 672;SER 674
1rs0A03SER 674
 
GLY 672;SER 674
1rtkA03SER 674
 
GLY 672;SER 674
1dleA02SER 195
 
GLY 193;SER 195
1dleB02SER 195
 
GLY 193;SER 195
2ok5A06SER 674
 
GLY 672;SER 674
2winI03SER 674
 
GLY 672;SER 674
2winJ03SER 674
 
GLY 672;SER 674
2winK03SER 674
 
GLY 672;SER 674
2winL03SER 674
 
GLY 672;SER 674
2xwbF06SER 674
 
GLY 672;SER 674
2xwbH06SER 674
 
GLY 672;SER 674
2xwjI06SER 674
 
GLY 672;SER 674
2xwjJ06SER 674
 
GLY 672;SER 674
2xwjK06SER 674
 
GLY 672;SER 674
2xwjL06SER 674
 
GLY 672;SER 674
3hrzD06SER 674
 
GLY 672;SER 674
3hs0D06SER 674
 
GLY 672;SER 674
3hs0I06SER 674
 
GLY 672;SER 674
2winB01 
 
 
2winD01 
 
 
2winF01 
 
 
2winH01 
 
 
2xwbB01 
 
 
2xwbD01 
 
 
2xwjB01 
 
 
2xwjD01 
 
 
2xwjF01 
 
 
2xwjH01 
 
 
3hrzB01 
 
 
3hs0B01 
 
 
3hs0G01 
 
 
2winB02 
 
 
2winD02 
 
 
2winF02 
 
 
2winH02 
 
 
2xwbB02 
 
 
2xwbD02 
 
 
2xwjB02 
 
 
2xwjD02 
 
 
2xwjF02 
 
 
2xwjH02 
 
 
3hrzB02 
 
 
3hs0B02 
 
 
3hs0G02 
 
 
2winB03 
 
 
2winD03 
 
 
2winF03 
 
 
2winH03 
 
 
2xwbB03 
 
 
2xwbD03 
 
 
2xwjB03 
 
 
2xwjD03 
 
 
2xwjF03 
 
 
2xwjH03 
 
 
3hrzC01 
 
 
3hs0C01 
 
 
3hs0H01 
 
 
2winB04 
 
 
2winD04 
 
 
2winF04 
 
 
2winH04 
 
 
2xwbB04 
 
 
2xwbD04 
 
 
2xwjB04 
 
 
2xwjD04 
 
 
2xwjF04 
 
 
2xwjH04 
 
 
2winB05 
 
 
2winD05 
 
 
2winF05 
 
 
2winH05 
 
 
2xwbB05 
 
 
2xwbD05 
 
 
2xwjB05 
 
 
2xwjD05 
 
 
2xwjF05 
 
 
2xwjH05 
 
 
3hrzC02 
 
 
3hs0C02 
 
 
3hs0H02 
 
 
2winB06 
ASN 1641(Magnesium binding)
 
2winD06 
ASN 1641(Magnesium binding)
 
2winF06 
ASN 1641(Magnesium binding)
 
2winH06 
ASN 1641(Magnesium binding)
 
2xwbB06 
ASN 1641(Magnesium binding)
 
2xwbD06 
ASN 1641(Magnesium binding)
 
2xwjB06 
ASN 1641(Magnesium binding)
 
2xwjD06 
ASN 1641(Magnesium binding)
 
2xwjF06 
ASN 1641(Magnesium binding)
 
2xwjH06 
ASN 1641(Magnesium binding)
 
3hrzC03 
THR 1620(Magnesium binding)
 
3hs0C03 
 
 
3hs0H03 
 
 

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[16]Figure 5
[17]Figure 1
[20]Figure 1
[21]Figure 3
[22]Figure 6

references
[1]
PubMed ID6559201
JournalJ Immunol
Year1984
Volume132
Pages1430-4
AuthorsFishelson Z, Pangburn MK, Muller-Eberhard HJ
TitleCharacterization of the initial C3 convertase of the alternative pathway of human complement.
[2]
PubMed ID3638964
JournalBiochem J
Year1986
Volume235
Pages723-30
AuthorsPangburn MK, Muller-Eberhard HJ
TitleThe C3 convertase of the alternative pathway of human complement. Enzymic properties of the bimolecular proteinase.
[3]
PubMed ID3643213
JournalJ Biol Chem
Year1987
Volume262
Pages1519-25
AuthorsPryzdial EL, Isenman DE
TitleAlternative complement pathway activation fragment Ba binds to C3b. Evidence that formation of the factor B-C3b complex involves two discrete points of contact.
[4]
PubMed ID2433342
JournalJ Immunol
Year1987
Volume138
Pages1143-9
AuthorsUeda A, Kearney JF, Roux KH, Volanakis JE
TitleProbing functional sites on complement protein B with monoclonal antibodies. Evidence for C3b-binding sites on Ba.
[5]
PubMed ID3183384
JournalJ Immunol
Year1988
Volume141
Pages3895-901
AuthorsKinoshita T, Takata Y, Kozono H, Takeda J, Hong KS, Inoue K
TitleC5 convertase of the alternative complement pathway: covalent linkage between two C3b molecules within the trimolecular complex enzyme.
[6]
PubMed ID7649982
JournalJ Biol Chem
Year1995
Volume270
Pages19716-22
AuthorsHourcade DE, Wagner LM, Oglesby TJ
TitleAnalysis of the short consensus repeats of human complement factor B by site-directed mutagenesis.
[7]
PubMed ID9642242
JournalJ Biol Chem
Year1998
Volume273
Pages16828-35
AuthorsRawal N, Pangburn MK
TitleC5 convertase of the alternative pathway of complement. Kinetic analysis of the free and surface-bound forms of the enzyme.
[8]
CommentsX-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 467-764.
PubMed ID10637221
JournalEMBO J
Year2000
Volume19
Pages164-73
AuthorsJing H, Xu Y, Carson M, Moore D, Macon KJ, Volanakis JE, Narayana SV
TitleNew structural motifs on the chymotrypsin fold and their potential roles in complement factor B.
Related PDB1dle
Related UniProtKBP00751
[9]
PubMed ID10617628
JournalJ Biol Chem
Year2000
Volume275
Pages378-85
AuthorsXu Y, Circolo A, Jing H, Wang Y, Narayana SV, Volanakis JE
TitleMutational analysis of the primary substrate specificity pocket of complement factor B. Asp(226) is a major structural determinant for p(1)-Arg binding.
[10]
PubMed ID10640753
JournalJ Immunol
Year2000
Volume164
Pages1379-85
AuthorsRawal N, Pangburn MK
TitleFunctional role of the noncatalytic subunit of complement C5 convertase.
[11]
PubMed ID10966820
JournalJ Mol Biol
Year2000
Volume301
Pages1267-85
AuthorsHinshelwood J, Perkins SJ
TitleConformational changes during the assembly of factor B from its domains by (1)H NMR spectroscopy and molecular modelling: their relevance to the regulation of factor B activity.
[12]
PubMed ID11414354
JournalImmunol Rev
Year2001
Volume180
Pages123-35
AuthorsXu Y, Narayana SV, Volanakis JE
TitleStructural biology of the alternative pathway convertase.
[13]
PubMed ID11367526
JournalInt Immunopharmacol
Year2001
Volume1
Pages415-22
AuthorsRawal N, Pangburn MK
TitleStructure/function of C5 convertases of complement.
[14]
PubMed ID11160326
JournalJ Immunol
Year2001
Volume166
Pages2635-42
AuthorsRawal N, Pangburn M
TitleFormation of high-affinity C5 convertases of the alternative pathway of complement.
[15]
PubMed ID12440962
JournalBiochem Soc Trans
Year2002
Volume30
Pages1006-10
AuthorsPangburn MK, Rawal N
TitleStructure and function of complement C5 convertase enzymes.
[16]
PubMed ID12475199
JournalChem Rev
Year2002
Volume102
Pages4501-24
AuthorsHedstrom L
TitleSerine protease mechanism and specificity.
[17]
PubMed ID15068800
JournalMol Cell
Year2004
Volume14
Pages17-28
AuthorsPonnuraj K, Xu Y, Macon K, Moore D, Volanakis JE, Narayana SV
TitleStructural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase.
Related PDB1rrk,1rs0,1rtk
Related UniProtKBP00751
[18]
PubMed ID15016353
JournalStructure
Year2004
Volume12
Pages371-8
AuthorsBhattacharya AA, Lupher ML Jr, Staunton DE, Liddington RC
TitleCrystal structure of the A domain from complement factor B reveals an integrin-like open conformation.
Related PDB1q0p
Related UniProtKBP00751
[19]
PubMed ID16301317
JournalJ Biol Chem
Year2006
Volume281
Pages2128-32
AuthorsHourcade DE
TitleThe role of properdin in the assembly of the alternative pathway C3 convertases of complement.
[20]
PubMed ID17310251
JournalNat Struct Mol Biol
Year2007
Volume14
Pages224-8
AuthorsMilder FJ, Gomes L, Schouten A, Janssen BJ, Huizinga EG, Romijn RA, Hemrika W, Roos A, Daha MR, Gros P
TitleFactor B structure provides insights into activation of the central protease of the complement system.
Related PDB2ok5
Related UniProtKBP00751
[21]
PubMed ID18064050
JournalNat Rev Immunol
Year2008
Volume8
Pages48-58
AuthorsGros P, Milder FJ, Janssen BJ
TitleComplement driven by conformational changes.
[22]
PubMed ID19574954
JournalEMBO J
Year2009
Volume28
Pages2469-78
AuthorsJanssen BJ, Gomes L, Koning RI, Svergun DI, Koster AJ, Fritzinger DC, Vogel CW, Gros P
TitleInsights into complement convertase formation based on the structure of the factor B-cobra venom factor complex.
Related PDB3hrz,3hs0
Related UniProtKBP00751,Q91132
[23]
PubMed ID19890040
JournalJ Immunol
Year2009
Volume183
Pages7347-51
AuthorsTorreira E, Tortajada A, Montes T, Rodriguez de Cordoba S, Llorca O
TitleCoexistence of closed and open conformations of complement factor B in the alternative pathway C3bB(Mg2+) proconvertase.
[24]
PubMed ID19503103
JournalNat Immunol
Year2009
Volume10
Pages721-7
AuthorsRooijakkers SH, Wu J, Ruyken M, van Domselaar R, Planken KL, Tzekou A, Ricklin D, Lambris JD, Janssen BJ, van Strijp JA, Gros P
TitleStructural and functional implications of the alternative complement pathway C3 convertase stabilized by a staphylococcal inhibitor.
Related PDB2win
Related UniProtKBP00751,P01024
[25]
PubMed ID19136636
JournalProc Natl Acad Sci U S A
Year2009
Volume106
Pages882-7
AuthorsTorreira E, Tortajada A, Montes T, Rodriguez de Cordoba S, Llorca O
Title3D structure of the C3bB complex provides insights into the activation and regulation of the complement alternative pathway convertase.
[26]
PubMed ID21205667
JournalScience
Year2010
Volume330
Pages1816-20
AuthorsForneris F, Ricklin D, Wu J, Tzekou A, Wallace RS, Lambris JD, Gros P
TitleStructures of C3b in complex with factors B and D give insight into complement convertase formation.
Related PDB2xwb,2xwj

comments
This enzyme belongs to peptidase family-S1.
There are three types of activation of complement system: the classical pathway, the alternative pathway, or the lectin pathway (see [25]). The formation of C3-convertases is common to each pathway: C3bBb in the alternative pathway; C4b2a in the classical pathway/lectin pathway (see [25]). This enzyme corresponds to the C3-convertase in the alternative pathway.
This enzyme is composed of C-terminal fragment of complement factor B, Bb (residues 260-764 of uniprot;P00751), and C-terminal chain of complement factor C3, C3b (residues 749-1663 of uniprot;P01024) (see [21]). Bb contains catalytic domain of serine protease, whereas C3b is involved in covalent attachment to target surfaces. When Bb binds one unit of C3b to form C3bBb complex, this enzyme can activate C3 to produce C3a and C3b as C3 convertase. In contrast, this enzyme can also activate complement factor C5 to produce C5a and C5b as C5 convertase, if Bb binds two units of C3b to generate (C3b)2Bb complex.
Moreover, factor B is cleaved to generate Bb by factor D (EC 3.4.21.46; D00429 in EzCatDB), when it is in complex with complement subcomponent C3b or with cobra venom factor (see [21]). According to the literature [22], the cobra venom factor (Uniprot;Q91132) is a potent homologue of C3b that generates more stable convertases.
Since this enzyme has got the same catalytic site as trypsin, it must catalyze the trypsin-like reaction.

createdupdated
2011-05-092011-08-27


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