EzCatDB: M00344
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DB codeM00344
RLCP classification3.103.130000.1162 : Transfer
CATH domainDomain 12.30.29.30 : PH-domain like
Domain 2-.-.-.-
Domain 3-.-.-.-
Domain 43.30.505.10 : SHC Adaptor Protein
Domain 53.30.200.20 : Phosphorylase Kinase; domain 1
Domain 61.10.510.10 : Transferase(Phosphotransferase); domain 1Catalytic domain
E.C.2.7.10.2

CATH domainRelated DB codes (homologues)
1.10.510.10 : Transferase(Phosphotransferase); domain 1M00125,M00124,M00131,T00224,M00127,M00129,M00130,M00132,M00136,M00196,M00197,M00198,M00304,M00323,M00325,M00326,M00327,M00328,M00329,M00330,M00331,M00332,M00333,M00335,M00339
2.30.29.30 : PH-domain likeM00125,M00183,M00118
3.30.200.20 : Phosphorylase Kinase; domain 1M00125,M00124,M00131,T00224,M00127,M00129,M00130,M00132,M00136,M00196,M00197,M00198,M00304,M00323,M00325,M00326,M00327,M00328,M00329,M00330,M00331,M00332,M00333,M00335,M00339,D00298
3.30.505.10 : SHC Adaptor ProteinM00183,M00043,M00130,M00148,T00256,M00304,M00333,M00339,T00221

Enzyme Name
UniProtKBKEGG

P51813
Protein nameCytoplasmic tyrosine-protein kinase BMXNon-specific protein-tyrosine kinase
ATP:protein-tyrosine O-phosphotransferase (ambiguous)
Bruton's tyrosine kinase
BMX
Cytoplasmic protein tyrosine kinase
ETK
Ntk
SynonymsEC 2.7.10.2
Bone marrow tyrosine kinase gene in chromosome X protein
Epithelial and endothelial tyrosine kinase
ETK
NTK38
RefSeqNP_001712.1 (Protein)
NM_001721.6 (DNA/RNA sequence)
NP_975010.1 (Protein)
NM_203281.2 (DNA/RNA sequence)
PfamPF00779 (BTK)
PF00169 (PH)
PF07714 (Pkinase_Tyr)
PF00017 (SH2)
[Graphical view]


UniProtKB:Accession NumberP51813
Entry nameBMX_HUMAN
ActivityATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SubunitInteracts with BCAR1, CAV1, MYD88, PTK2/FAK1, RUFY1, RUFY2, STAT3, TIRAP and TNFRSF1B.
Subcellular locationCytoplasm. Note: Localizes to the edges of spreading cells when complexed with BCAR1.
CofactorBinds 1 zinc ion per subunit (By similarity).

Compound table: links to PDB-related databases & PoSSuM

CofactorsSubstratesProducts
KEGG-idC00305C00002C00585C00008C01167
CompoundMagnesiumATP[protein]-L-tyrosineADP[protein]-L-tyrosine phosphate
Typedivalent metal (Ca2+, Mg2+)amine group,nucleotidearomatic ring (only carbon atom),peptide/proteinamine group,nucleotidearomatic ring (only carbon atom),peptide/protein,phosphate group/phosphate ion
ChEBI18420
15422

16761

PubChem888
5957

6022

             
2ys2AUnboundUnboundUnboundUnboundUnbound
2ekxA00UnboundUnboundUnboundUnboundUnbound
3sxrA01UnboundUnboundUnboundUnboundUnbound
3sxrB01UnboundUnboundUnboundUnboundUnbound
3sxsA01UnboundUnboundUnboundUnboundUnbound
3sxrA02UnboundUnboundUnboundUnboundUnbound
3sxrB02UnboundUnboundUnboundUnboundUnbound
3sxsA02UnboundUnboundUnboundUnboundUnbound

Active-site residues
pdbCatalytic residuesCofactor-binding residues
          
2ys2A               
 
2ekxA00               
 
3sxrA01               
 
3sxrB01               
 
3sxsA01               
 
3sxrA02ASP 536;ARG 540
ASN 541;ASP 554(Magnesium binding)
3sxrB02ASP 536;ARG 540
ASN 541;ASP 554(Magnesium binding)
3sxsA02ASP 536;ARG 540
ASN 541;ASP 554(Magnesium binding)

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[1]Figure 2

references
[1]
CommentsREVIEW ON FUNCTION.
PubMed ID11114746
JournalOncogene
Year2000
Volume19
Pages5651-61
AuthorsQiu Y, Kung HJ
TitleSignaling network of the Btk family kinases.
[2]
PubMed ID11331870
JournalNat Cell Biol
Year2001
Volume3
Pages439-44
AuthorsChen R, Kim O, Li M, Xiong X, Guan JL, Kung HJ, Chen H, Shimizu Y, Qiu Y
TitleRegulation of the PH-domain-containing tyrosine kinase Etk by focal adhesion kinase through the FERM domain.
[3]
PubMed ID14532277
JournalJ Biol Chem
Year2003
Volume278
Pages51267-76
AuthorsZhang R, Xu Y, Ekman N, Wu Z, Wu J, Alitalo K, Min W
TitleEtk/Bmx transactivates vascular endothelial growth factor 2 and recruits phosphatidylinositol 3-kinase to mediate the tumor necrosis factor-induced angiogenic pathway.
[4]
PubMed ID18292575
JournalJ Immunol
Year2008
Volume180
Pages3485-91
AuthorsSemaan N, Alsaleh G, Gottenberg JE, Wachsmann D, Sibilia J
TitleEtk/BMX, a Btk family tyrosine kinase, and Mal contribute to the cross-talk between MyD88 and FAK pathways.
[5]
PubMed ID21883956
JournalChem Biol Drug Des
Year2011
Volume78
Pages739-48
AuthorsMuckelbauer J, Sack JS, Ahmed N, Burke J, Chang CY, Gao M, Tino J, Xie D, Tebben AJ
TitleX-ray crystal structure of bone marrow kinase in the x chromosome: a Tec family kinase.
Related PDB3sxr,3sxs
Related UniProtKBP51813

comments
This enzyme belongs to Tec family of non-receptor tyrosine kinases.
This enzyme is composed of the N-terminal pleckstrin homology (PH) domain, BTK-type zinc finger motif, an unknown domain, which can be Src homology 3 (SH3) domain, Src homology 2 (SH2) domain, and the C-terminal kinase domain (see [1]).
The catalytic domain of this enzyme is homologous to Proto-oncogene tyrosine-protein kinase ABL1 (M00130 in EzCatDB), with common active-site residues.

createdupdated
2012-06-192013-01-30
Copyright: Nozomi Nagano, JST & CBRC-AIST
Funded by PRESTO/Japan Science and Technology Corporation (JST) (December 2001 - November 2004)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005 - September 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2012 - March 2013)
Supported by the commission for the Development of Artificial Gene Synthesis Technology for Creating Innovative Biomaterial from the Ministry of Economy, Trade and Industry (METI) (October 2012 - )
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