|
CATH domain | Related DB codes (homologues) |
---|
3.20.20.70 : TIM Barrel | S00215,S00217,S00218,S00219,S00532,S00198,S00220,S00745,S00537,S00538,S00539,S00826,S00841,S00235,S00239,S00240,S00243,S00244,S00200,S00201,S00221,S00222,S00847,S00224,S00225,S00226,D00014,D00029,M00141,T00015,T00239,D00664,D00665,D00804,D00863,T00089 |
KEGG pathways | MAP code | Pathways |
---|
MAP00010 | Glycolysis / Gluconeogenesis | MAP00030 | Pentose phosphate pathway | MAP00051 | Fructose and mannose metabolism | MAP00710 | Carbon fixation in photosynthetic organisms |
UniProtKB:Accession Number | P04075 | P05062 | P00883 | P07764 | P14223 | P07752 |
---|
Entry name | ALDOA_HUMAN | ALDOB_HUMAN | ALDOA_RABIT | ALF_DROME | ALF_PLAFA | ALF_TRYBB |
---|
Activity | D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. | D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. | D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. | D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. | D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. | D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. |
---|
Subunit | Homotetramer. | Homotetramer. | Tetramer. | Homotetramer. | Homotetramer. |
|
---|
Subcellular location |
|
|
|
|
| Glycosome. |
---|
Cofactor |
|
|
|
|
|
|
---|
Compound table: links to PDB-related databases & PoSSuM |
---|
| Substrates | Products | intermediates |
---|
KEGG-id | C00354 | C00111 | C00118 |
|
---|
Compound | D-Fructose 1,6-bisphosphate | Glycerone phosphate | D-Glyceraldehyde 3-phosphate | Intermediate |
---|
Type | carbohydrate,phosphate group/phosphate ion | carbohydrate,phosphate group/phosphate ion | carbohydrate,phosphate group/phosphate ion |
|
---|
ChEBI | 37736
| 16108
| 29052
|
|
---|
PubChem | 172313
| 668
| 439168
|
|
---|
| | | | | | | | | | | |
---|
1a5cA |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1a5cB |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1adoA |  |  |  |  |  |  |  | Unbound | Bound:13P | Unbound | Unbound |
---|
1adoB |  |  |  |  |  |  |  | Unbound | Bound:13P | Unbound | Unbound |
---|
1adoC |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1adoD |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1aldA |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1epxA |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1epxB |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1epxC |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1epxD |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1ewdA |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1ewdB |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1ewdC |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1ewdD |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1eweA |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1eweB |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1eweC |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1eweD |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1ewgA |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1ewgB |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1ewgC |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1ewgD |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1ex5A |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1ex5B |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1ex5C |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1ex5D |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1f2jA |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1fbaA |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1fbaB |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1fbaC |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1fbaD |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1j4eA |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Intermediate-bound:13P |
---|
1j4eB |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Intermediate-bound:13P |
---|
1j4eC |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Intermediate-bound:13P |
---|
1j4eD |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Intermediate-bound:13P |
---|
1qo5A |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5B |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5C |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5D |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5E |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5F |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5G |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5H |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5I |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5J |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5K |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5L |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5M |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5N |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5O |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5P |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5Q |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
1qo5R |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
2aldA |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
4aldA |  |  |  |  |  |  |  | Bound:2FP | Unbound | Unbound | Unbound |
---|
6aldA |  |  |  |  |  |  |  | Bound:2FP | Unbound | Unbound | Unbound |
---|
6aldB |  |  |  |  |  |  |  | Bound:2FP | Unbound | Unbound | Unbound |
---|
6aldC |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
6aldD |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound |
---|
References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
---|
[11] | SCHEME 3, p.135-136 |
| [16] | SCHEME 1 |
| [26] | Fig.1 |
| [32] | Scheme 2 |
| [33] | Fig.2, Fig.3, p.38-39 | 6 | [34] | Scheme 1, p.12294, p.12996-12997 | 5 | [40] | p.36-38 |
| [41] | Scheme 1, p.2088-2089 | 6 | [43] | p.12662-12663 |
| [45] | p.295, Table 3 |
| [46] | p.823 |
| [47] | p.1149-1150 |
| [51] | Fig.5, p.13874 | 10 | [54] | p.9482 |
|
references | [1] |
---|
PubMed ID | 4607364 |
---|
Journal | Biochemistry |
---|
Year | 1974 |
---|
Volume | 13 |
---|
Pages | 4371-5 |
---|
Authors | Heron EJ, Caprioli RM |
---|
Title | 18O studies of the mechanisms of yeast and muscle aldolases. |
---|
[2] |
---|
PubMed ID | 954748 |
---|
Journal | Eur J Biochem |
---|
Year | 1976 |
---|
Volume | 66 |
---|
Pages | 95-104 |
---|
Authors | Lowe G, Pratt RF |
---|
Title | Proton exchange of the pro-S hydrogen at C-1 in dihydroxyacetone phosphate, D-fructose 1,6-bisphosphate and D-fructose 1-phosphate catalysed by rabbit-muscle aldolase. |
---|
[3] |
---|
Comments | ACTIVE SITE. |
---|
Medline ID | 76190154 |
---|
PubMed ID | 5453 |
---|
Journal | J Biol Chem |
---|
Year | 1976 |
---|
Volume | 251 |
---|
Pages | 3057-62 |
---|
Authors | Hartman FC, Brown JP |
---|
Title | Affinity labeling of a previously undetected essential lysyl residue in class I fructose bisphosphate aldolase. |
---|
Related UniProtKB | P00883 |
---|
[4] |
---|
PubMed ID | 911752 |
---|
Journal | Biochemistry |
---|
Year | 1977 |
---|
Volume | 16 |
---|
Pages | 3988-94 |
---|
Authors | Pratt RF |
---|
Title | Rabbit muscle aldolase catalyzed proton exchange of hydroxyacetone phosphate with solvent. |
---|
[5] |
---|
PubMed ID | 18166 |
---|
Journal | Biochemistry |
---|
Year | 1977 |
---|
Volume | 16 |
---|
Pages | 2966-71 |
---|
Authors | Strapazon E, Steck TL |
---|
Title | Interaction of the aldolase and the membrane of human erythrocytes. |
---|
[6] |
---|
PubMed ID | 540038 |
---|
Journal | Biochem J |
---|
Year | 1979 |
---|
Volume | 183 |
---|
Pages | 663-7 |
---|
Authors | Stewart M, Morton DJ, Clarke FM |
---|
Title | Changes associated with glycolytic-enzyme binding in the equatorial X-ray-diffraction pattern of glycerinated rabbit psoas muscle. |
---|
[7] |
---|
Comments | SUBSTRATE-BINDING SITE. |
---|
Medline ID | 80046697 |
---|
PubMed ID | 499203 |
---|
Journal | Eur J Biochem |
---|
Year | 1979 |
---|
Volume | 99 |
---|
Pages | 309-13 |
---|
Authors | Patthy L, Varadi A, Thesz J, Kovacs K |
---|
Title | Identification of the C-1-phosphate-binding arginine residue of rabbit-muscle aldolase. Isolation of 1,2-cyclohexanedione-labeled peptide by chemisorption chromatography. |
---|
Related UniProtKB | P00883 |
---|
[8] |
---|
PubMed ID | 293723 |
---|
Journal | Proc Natl Acad Sci U S A |
---|
Year | 1979 |
---|
Volume | 76 |
---|
Pages | 6323-5 |
---|
Authors | Pontremoli S, Melloni E, Salamino F, Sparatore B, Michetti M, Horecker BL |
---|
Title | Changes in activity of fructose-1,6-bisphosphate aldolase in livers of fasted rabbits and accumulation of crossreacting immune material. |
---|
[9] |
---|
PubMed ID | 7385224 |
---|
Journal | Tohoku J Exp Med |
---|
Year | 1980 |
---|
Volume | 130 |
---|
Pages | 143-52 |
---|
Authors | Nakashima K, Ohtsuki M, Tuboi S |
---|
Title | Membrane-bound fructose 1,6-bisphosphate aldolase: catalytic activity and mechanisms of desorption. |
---|
[10] |
---|
PubMed ID | 6115420 |
---|
Journal | Philos Trans R Soc Lond B Biol Sci |
---|
Year | 1981 |
---|
Volume | 293 |
---|
Pages | 209-14 |
---|
Authors | Millar JR, Shaw PJ, Stammers DK, Watson HC |
---|
Title | The low-resolution structure of human muscle aldolase. |
---|
[11] |
---|
PubMed ID | 6115413 |
---|
Journal | Philos Trans R Soc Lond B Biol Sci |
---|
Year | 1981 |
---|
Volume | 293 |
---|
Pages | 131-43 |
---|
Authors | Rose IA |
---|
Title | Chemistry of proton abstraction by glycolytic enzymes (aldolase, isomerases and pyruvate kinase). |
---|
[12] |
---|
PubMed ID | 7092833 |
---|
Journal | Biochem J |
---|
Year | 1982 |
---|
Volume | 202 |
---|
Pages | 589-602 |
---|
Authors | Brindle KM, Brown FF, Campbell ID, Foxall DL, Simpson RJ |
---|
Title | A 1H n.m.r. study of isotope exchange catalysed by glycolytic enzymes in the human erythrocyte. |
---|
[13] |
---|
PubMed ID | 6838539 |
---|
Journal | Biochem Biophys Res Commun |
---|
Year | 1983 |
---|
Volume | 110 |
---|
Pages | 578-83 |
---|
Authors | Grazi E, Trombetta G, Lanzara V |
---|
Title | Fructose-1,6-bisphosphate-aldolase from rabbit muscle. Half of the sites reactivity at low temperature. |
---|
[14] |
---|
PubMed ID | 6679317 |
---|
Journal | Biochem Int |
---|
Year | 1983 |
---|
Volume | 6 |
---|
Pages | 53-61 |
---|
Authors | Kelkar SM, Kaklij GS |
---|
Title | Mechanism of aldolase binding to erythrocyte membrane: Part II. Kinetic aspects. |
---|
[15] |
---|
PubMed ID | 6406231 |
---|
Journal | Eur J Biochem |
---|
Year | 1983 |
---|
Volume | 133 |
---|
Pages | 433-7 |
---|
Authors | Ovadi J, Mohamed Osman IR, Batke J |
---|
Title | Interaction of the dissociable glycerol-3-phosphate dehydrogenase and fructose-1,6-bisphosphate aldolase. Quantitative analysis by an extrinsic fluorescence probe. |
---|
[16] |
---|
PubMed ID | 6486803 |
---|
Journal | Arch Biochem Biophys |
---|
Year | 1984 |
---|
Volume | 233 |
---|
Pages | 595-602 |
---|
Authors | Grazi E, Trombetta G |
---|
Title | Fructose-1,6-bisphosphate aldolase from rabbit muscle: different catalytic behavior of the dihydroxyacetone phosphate binding sites at low temperature. |
---|
[17] |
---|
PubMed ID | 6487321 |
---|
Journal | Biochem Biophys Res Commun |
---|
Year | 1984 |
---|
Volume | 123 |
---|
Pages | 1069-75 |
---|
Authors | Sygusch J, Lehoux L, Beaudry D |
---|
Title | Extreme X-ray sensitive modification of type I aldolases by blue dye ligand chromatography. |
---|
[18] |
---|
PubMed ID | 4026283 |
---|
Journal | Appl Biochem Biotechnol |
---|
Year | 1985 |
---|
Volume | 11 |
---|
Pages | 91-100 |
---|
Authors | Abraham M, Horvath L, Simon M, Szajani B, Boross L |
---|
Title | Characterization and comparison of soluble and immobilized pig muscle aldolases. |
---|
[19] |
---|
PubMed ID | 4066671 |
---|
Journal | J Biol Chem |
---|
Year | 1985 |
---|
Volume | 260 |
---|
Pages | 15286-90 |
---|
Authors | Sygusch J, Boulet H, Beaudry D |
---|
Title | Structure of rabbit muscle aldolase at low resolution. |
---|
[20] |
---|
PubMed ID | 3942758 |
---|
Journal | Biochim Biophys Acta |
---|
Year | 1986 |
---|
Volume | 869 |
---|
Pages | 185-91 |
---|
Authors | Swain MS, Lebherz HG |
---|
Title | Specific, limited tryptic modification of wheat-germ fructose-bisphosphate aldolase subunits: destruction of catalytic activity but not of ability to establish precise subunit-subunit recognition. |
---|
[21] |
---|
PubMed ID | 3569280 |
---|
Journal | Eur J Biochem |
---|
Year | 1987 |
---|
Volume | 164 |
---|
Pages | 655-9 |
---|
Authors | Vertessy B, Ovadi J |
---|
Title | A simple approach to detect active-site-directed enzyme-enzyme interactions. The aldolase/glycerol-phosphate-dehydrogenase enzyme system. |
---|
[22] |
---|
Comments | VARIANT HEMOLYTIC ANEMIA GLY-128. |
---|
Medline ID | 88068641 |
---|
PubMed ID | 2825199 |
---|
Journal | Proc Natl Acad Sci U S A |
---|
Year | 1987 |
---|
Volume | 84 |
---|
Pages | 8623-7 |
---|
Authors | Kishi H, Mukai T, Hirono A, Fujii H, Miwa S, Hori K |
---|
Title | Human aldolase A deficiency associated with a hemolytic anemia: thermolabile aldolase due to a single base mutation. |
---|
Related UniProtKB | P04075 |
---|
[23] |
---|
PubMed ID | 2556962 |
---|
Journal | Appl Biochem Biotechnol |
---|
Year | 1989 |
---|
Volume | 22 |
---|
Pages | 223-35 |
---|
Authors | Horvath L, Abraham M, Boross L, Szajani B |
---|
Title | Immobilization of pig muscle aldolase on a silica-based support. |
---|
[24] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS). |
---|
Medline ID | 90242948 |
---|
PubMed ID | 2335208 |
---|
Journal | FEBS Lett |
---|
Year | 1990 |
---|
Volume | 262 |
---|
Pages | 282-6 |
---|
Authors | Gamblin SJ, Cooper B, Millar JR, Davies GJ, Littlechild JA, Watson HC |
---|
Title | The crystal structure of human muscle aldolase at 3.0 A resolution. |
---|
Related UniProtKB | P04075 |
---|
[25] |
---|
Comments | VARIANT HEMOLYTIC ANEMIA GLY-128. |
---|
Medline ID | 91035340 |
---|
PubMed ID | 2229018 |
---|
Journal | J Biochem (Tokyo) |
---|
Year | 1990 |
---|
Volume | 108 |
---|
Pages | 153-7 |
---|
Authors | Takasaki Y, Takahashi I, Mukai T, Hori K |
---|
Title | Human aldolase A of a hemolytic anemia patient with Asp-128----Gly substitution: characteristics of an enzyme generated in E. coli transfected with the expression plasmid pHAAD128G. |
---|
Related UniProtKB | P04075 |
---|
[26] |
---|
PubMed ID | 1814134 |
---|
Journal | Acta Biochim Pol |
---|
Year | 1991 |
---|
Volume | 38 |
---|
Pages | 407-21 |
---|
Authors | Kochman M, Dobryszycki P |
---|
Title | Topography and conformational changes of fructose-1,6-bisphosphate aldolase. |
---|
[27] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). |
---|
Medline ID | 92070498 |
---|
PubMed ID | 1959612 |
---|
Journal | FEBS Lett |
---|
Year | 1991 |
---|
Volume | 292 |
---|
Pages | 237-42 |
---|
Authors | Hester G, Brenner-Holzach O, Rossi FA, Struck-Donatz M, Winterhalter KH, Smit JD, Piontek K |
---|
Title | The crystal structure of fructose-1,6-bisphosphate aldolase from Drosophila melanogaster at 2.5 A resolution. |
---|
Related PDB | 1fba |
---|
Related UniProtKB | P07764 |
---|
[28] |
---|
PubMed ID | 1894606 |
---|
Journal | J Biol Chem |
---|
Year | 1991 |
---|
Volume | 266 |
---|
Pages | 17099-105 |
---|
Authors | Berthiaume L, Loisel TP, Sygusch J |
---|
Title | Carboxyl terminus region modulates catalytic activity of recombinant maize aldolase. |
---|
[29] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). |
---|
Medline ID | 91278081 |
---|
PubMed ID | 2056525 |
---|
Journal | J Mol Biol |
---|
Year | 1991 |
---|
Volume | 219 |
---|
Pages | 573-6 |
---|
Authors | Gamblin SJ, Davies GJ, Grimes JM, Jackson RM, Littlechild JA, Watson HC |
---|
Title | Activity and specificity of human aldolases. |
---|
Related PDB | 1ald |
---|
Related UniProtKB | P04075 |
---|
[30] |
---|
PubMed ID | 1417758 |
---|
Journal | Biochem J |
---|
Year | 1992 |
---|
Volume | 286 |
---|
Pages | 977-9 |
---|
Authors | Vertessy BG, Vas M |
---|
Title | Metabolite channeling versus free diffusion: reinterpretation of aldolase-catalysed inactivation of glyceraldehyde-3-phosphate dehydrogenase. |
---|
[31] |
---|
PubMed ID | 1577806 |
---|
Journal | J Biol Chem |
---|
Year | 1992 |
---|
Volume | 267 |
---|
Pages | 9713-7 |
---|
Authors | Rae C, Bubb WA, Kuchel PW |
---|
Title | Aldolase-catalyzed diketone phosphate formation from oxoaldehydes. NMR studies and metabolic significance. |
---|
[32] |
---|
PubMed ID | 8513801 |
---|
Journal | Eur J Biochem |
---|
Year | 1993 |
---|
Volume | 214 |
---|
Pages | 515-9 |
---|
Authors | Gupta S, Hollenstein R, Kochhar S, Christen P |
---|
Title | Paracatalytic self-inactivation of fructose-1,6-bisphosphate aldolase. Structure of the crosslink formed at the active site. |
---|
[33] |
---|
PubMed ID | 8488556 |
---|
Journal | Trends Biochem Sci |
---|
Year | 1993 |
---|
Volume | 18 |
---|
Pages | 36-9 |
---|
Authors | Littlechild JA, Watson HC |
---|
Title | A data-based reaction mechanism for type I fructose bisphosphate aldolase. |
---|
[34] |
---|
PubMed ID | 7918450 |
---|
Journal | Biochemistry |
---|
Year | 1994 |
---|
Volume | 33 |
---|
Pages | 12291-7 |
---|
Authors | Morris AJ, Tolan DR |
---|
Title | Lysine-146 of rabbit muscle aldolase is essential for cleavage and condensation of the C3-C4 bond of fructose 1,6-bis(phosphate). |
---|
[35] |
---|
PubMed ID | 8527430 |
---|
Journal | Biochemistry |
---|
Year | 1995 |
---|
Volume | 34 |
---|
Pages | 16574-84 |
---|
Authors | Schneider ML, Post CB |
---|
Title | Solution structure of a band 3 peptide inhibitor bound to aldolase: a proposed mechanism for regulating binding by tyrosine phosphorylation. |
---|
[36] |
---|
PubMed ID | 8636111 |
---|
Journal | J Biol Chem |
---|
Year | 1996 |
---|
Volume | 271 |
---|
Pages | 6861-5 |
---|
Authors | Wang J, Morris AJ, Tolan DR, Pagliaro L |
---|
Title | The molecular nature of the F-actin binding activity of aldolase revealed with site-directed mutants. |
---|
[37] |
---|
PubMed ID | 8643582 |
---|
Journal | Proc Natl Acad Sci U S A |
---|
Year | 1996 |
---|
Volume | 93 |
---|
Pages | 5374-9 |
---|
Authors | Beernink PT, Tolan DR |
---|
Title | Disruption of the aldolase A tetramer into catalytically active monomers. |
---|
[38] |
---|
Comments | X-ray crystallography |
---|
Journal | Protein Pept Lett |
---|
Year | 1996 |
---|
Volume | 3 |
---|
Pages | 207-12 |
---|
Authors | Dalby A, Rawas A, Watson HC, Littlechild JA |
---|
Title | Crystallisation and Preliminary X-Ray Diffraction Studies on Human Liver Aldolase. |
---|
Related PDB | 1qo5 |
---|
[39] |
---|
PubMed ID | 9325270 |
---|
Journal | J Biol Chem |
---|
Year | 1997 |
---|
Volume | 272 |
---|
Pages | 25542-6 |
---|
Authors | Vertessy BG, Orosz F, Kovacs J, Ovadi J |
---|
Title | Alternative binding of two sequential glycolytic enzymes to microtubules. Molecular studies in the phosphofructokinase/aldolase/microtubule system. |
---|
[40] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS). |
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Medline ID | 97143309 |
---|
PubMed ID | 8989320 |
---|
Journal | Nat Struct Biol |
---|
Year | 1997 |
---|
Volume | 4 |
---|
Pages | 36-9 |
---|
Authors | Blom N, Sygusch J |
---|
Title | Product binding and role of the C-terminal region in class I D-fructose 1,6-bisphosphate aldolase. |
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Related PDB | 1ado |
---|
Related UniProtKB | P00883 |
---|
[41] |
---|
PubMed ID | 9405338 |
---|
Journal | Science |
---|
Year | 1997 |
---|
Volume | 278 |
---|
Pages | 2085-92 |
---|
Authors | Barbas CF 3rd, Heine A, Zhong G, Hoffmann T, Gramatikova S, Bjornestedt R, List B, Anderson J, Stura EA, Wilson IA, Lerner RA |
---|
Title | Immune versus natural selection: antibody aldolases with enzymic rates but broader scope. |
---|
[42] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS). |
---|
Medline ID | 98190013 |
---|
PubMed ID | 9521758 |
---|
Journal | Biochemistry |
---|
Year | 1998 |
---|
Volume | 37 |
---|
Pages | 4388-96 |
---|
Authors | Kim H, Certa U, Dobeli H, Jakob P, Hol WG |
---|
Title | Crystal structure of fructose-1,6-bisphosphate aldolase from the human malaria parasite Plasmodium falciparum. |
---|
Related PDB | 1a5c |
---|
Related UniProtKB | P14223 |
---|
[43] |
---|
Comments | X-ray crystallography |
---|
PubMed ID | 10504235 |
---|
Journal | Biochemistry |
---|
Year | 1999 |
---|
Volume | 38 |
---|
Pages | 12655-64 |
---|
Authors | Choi KH, Mazurkie AS, Morris AJ, Utheza D, Tolan DR, Allen KN |
---|
Title | Structure of a fructose-1,6-bis(phosphate) aldolase liganded to its natural substrate in a cleavage-defective mutant at 2.3 A(,). |
---|
Related PDB | 6ald |
---|
[44] |
---|
PubMed ID | 10336621 |
---|
Journal | Eur J Biochem |
---|
Year | 1999 |
---|
Volume | 262 |
---|
Pages | 371-6 |
---|
Authors | Pettersson H, Pettersson G |
---|
Title | Mechanism of metabolite transfer in coupled two-enzyme reactions involving aldolase. |
---|
[45] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS). |
---|
Medline ID | 99156067 |
---|
PubMed ID | 10048322 |
---|
Journal | Protein Sci |
---|
Year | 1999 |
---|
Volume | 8 |
---|
Pages | 291-7 |
---|
Authors | Dalby A, Dauter Z, Littlechild JA |
---|
Title | Crystal structure of human muscle aldolase complexed with fructose 1,6-bisphosphate: mechanistic implications. |
---|
Related PDB | 2ald,4ald |
---|
Related UniProtKB | P04075 |
---|
[46] |
---|
PubMed ID | 10970798 |
---|
Journal | Biochem J |
---|
Year | 2000 |
---|
Volume | 350 Pt 3 |
---|
Pages | 823-8 |
---|
Authors | Santamaria R, Esposito G, Vitagliano L, Race V, Paglionico I, Zancan L, Zagari A, Salvatore F |
---|
Title | Functional and molecular modelling studies of two hereditary fructose intolerance-causing mutations at arginine 303 in human liver aldolase. |
---|
[47] |
---|
PubMed ID | 10625657 |
---|
Journal | J Biol Chem |
---|
Year | 2000 |
---|
Volume | 275 |
---|
Pages | 1145-51 |
---|
Authors | Rellos P, Sygusch J, Cox TM |
---|
Title | Expression, purification, and characterization of natural mutants of human aldolase B. Role of quaternary structure in catalysis. |
---|
[48] |
---|
Comments | X-ray crystallography |
---|
PubMed ID | 10891264 |
---|
Journal | J Mol Biol |
---|
Year | 2000 |
---|
Volume | 300 |
---|
Pages | 697-707 |
---|
Authors | Chudzik DM, Michels PA, de Walque S, Hol WG |
---|
Title | Structures of type 2 peroxisomal targeting signals in two trypanosomatid aldolases. |
---|
Related PDB | 1epx,1f2j |
---|
Related UniProtKB | P07752 |
---|
[49] |
---|
PubMed ID | 10959854 |
---|
Journal | J Org Chem |
---|
Year | 2000 |
---|
Volume | 65 |
---|
Pages | 4529-31 |
---|
Authors | Chenevert R, Dasser M |
---|
Title | Chemoenzymatic synthesis of the microbial elicitor (-)-syringolide via a fructose 1,6-diphosphate aldolase-catalyzed condensation reaction. |
---|
[50] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) |
---|
PubMed ID | 11679716 |
---|
Journal | Acta Crystallogr D Biol Crystallogr |
---|
Year | 2001 |
---|
Volume | 57 |
---|
Pages | 1526-33 |
---|
Authors | Dalby AR, Tolan DR, Littlechild JA |
---|
Title | The structure of human liver fructose-1,6-bisphosphate aldolase. |
---|
Related UniProtKB | P05062 |
---|
[51] |
---|
PubMed ID | 11705376 |
---|
Journal | Biochemistry |
---|
Year | 2001 |
---|
Volume | 40 |
---|
Pages | 13868-75 |
---|
Authors | Choi KH, Shi J, Hopkins CE, Tolan DR, Allen KN |
---|
Title | Snapshots of catalysis: the structure of fructose-1,6-(bis)phosphate aldolase covalently bound to the substrate dihydroxyacetone phosphate. |
---|
Related PDB | 1j4e |
---|
[52] |
---|
PubMed ID | 11371431 |
---|
Journal | Biophys J |
---|
Year | 2001 |
---|
Volume | 80 |
---|
Pages | 2527-35 |
---|
Authors | Ouporov IV, Knull HR, Huber A, Thomasson KA |
---|
Title | Brownian dynamics simulations of aldolase binding glyceraldehyde 3-phosphate dehydrogenase and the possibility of substrate channeling. |
---|
[53] |
---|
PubMed ID | 12417303 |
---|
Journal | FEBS Lett |
---|
Year | 2002 |
---|
Volume | 531 |
---|
Pages | 152-6 |
---|
Authors | Esposito G, Vitagliano L, Santamaria R, Viola A, Zagari A, Salvatore F |
---|
Title | Structural and functional analysis of aldolase B mutants related to hereditary fructose intolerance. |
---|
[54] |
---|
Comments | X-ray crystallography |
---|
PubMed ID | 11779856 |
---|
Journal | J Biol Chem |
---|
Year | 2002 |
---|
Volume | 277 |
---|
Pages | 9474-83 |
---|
Authors | Maurady A, Zdanov A, de Moissac D, Beaudry D, Sygusch J |
---|
Title | A conserved glutamate residue exhibits multifunctional catalytic roles in D-fructose-1,6-bisphosphate aldolases. |
---|
Related PDB | 1ewd,1ewe,1ewg,1ex5 |
---|
[55] |
---|
PubMed ID | 11835505 |
---|
Journal | Proteins |
---|
Year | 2002 |
---|
Volume | 46 |
---|
Pages | 295-307 |
---|
Authors | Zabell AP, Post CB |
---|
Title | Docking multiple conformations of a flexible ligand into a protein binding site using NMR restraints. |
---|
[56] |
---|
PubMed ID | 15025449 |
---|
Journal | J Am Chem Soc |
---|
Year | 2004 |
---|
Volume | 126 |
---|
Pages | 3402-3 |
---|
Authors | Choi KH, Tolan DR |
---|
Title | Presteady-state kinetic evidence for a ring-opening activity in fructose-1,6-(bis)phosphate aldolase. |
---|
comments | This enzyme is class I aldolase. The class II aldolase (S00235 in EzCatDB) has got totally different mechanism with zinc ion at the active site. The literature [51] & [54] indicate different catalytic residues. Accoridng to the literature [51], Glu187 plays a catalytic role by protonating the C2-hydroxyl group during Schiff-base formation, and Asp33 is involved in C3-C4 bond cleavage. In contrast, Glu187 is involved in the bond cleavage, according to the literature [54]. (The active site structures support the literature [54].) According to the literature [51], [54] & [56], the catalytic reaction of this enzyme may proceeds as follows; (A) Eliminative double-bond formation; Ring-opening/hemiketal cleavage (B) Exchange of double-bonded atoms; Schiff-base formation (B1) Glu187 acts as a general base to abstract a proton from the sidechain of Lys229, enhancing its nucleophilicity (see [54]). Here, the function of Glu187 is modulated by Glu189. (B2) The activated Lys229 makes a nucleophilic attack on C2-carbonyl group, to form a carbinolamine intermediate. (Here, a proton must transfer from the sidechain amine of Lys229 to the C2-carbonyl oxygen, with a probable assistance by Glu187. Glu187 may protonate the C2-carbonyl oxygen, and then deprotonate the amine of Lys229.) (B3) The lone pair of Lys229 makes another attak on C2-carbon, whilst Glu187 acts as a general acid to protonate the eliminated hydroxyl group. This reaction leads to Schiff-base intermeidate formation. (C) Eliminative double-bond formation;C3-C4 bond cleavage (C1) Glu187 acts as a general base to deprotonate C4-hydroxyl group, leading to the C3-C4 bond cleavage (see [54]). Here, the electron sink (Schiff-base) formed at C2 facilitate the deprotonation, and formation of C2=C3 double-bond, or ketamine (or enamine) intermediate. (According to the literature [1], instead of Glu187, Asp33 acts as the base.) (C2) The first product, G3P, is released from the active site. (D) Isomerization; Shift of double-bond (D1) The lone pair at nitrogen atom of Lys229 makes an attack on C2 carbon, to form Schiff-base again, which leads to C3-carbanion intermediate. The carbanion seems to be stabilized by the protonated sidechain of Lys146. (D2) Asp33 protonates the carbanion, to form an imine intermediate. (E) Exchange of double-bonded atoms; Schiff-base deformation (E1) Glu187 acts as a general base, to activate a water molecule. (E2) The activated water makes a nucleophilic attack on the C2 carbon, to form a carbinolamine intermeidate. (Here, a proton must transfer from the hydroxyl group to the sidechain amine of Lys229, with a probable assistance by Glu187. Glu187 may protonate the sidechain of Lys229, and then deprotonate the hydroxyl group.) (E3) The lone pair at the hydroxyl group of carbinolamine makes a nucleophilic attack on the C2 carbon again, to release the sidechain of Lys229. (E4) Unprotonated Lys229 must be protonated by Glu187.
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created | updated |
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2004-05-24 | 2009-02-26 |
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