|
CATH domain | Related DB codes (homologues) |
---|
3.20.20.80 : TIM Barrel | S00202,S00210,S00748,S00906,S00907,S00911,S00912,S00915,M00134,M00160,D00479,S00204,S00206,S00207,S00203,S00208,S00209,S00211,S00213,S00214,M00113,T00307,D00165,D00166,D00169,D00176,D00501,D00502,D00503,D00844,D00861,D00864,M00026,M00112,M00193,M00346,T00057,T00062,T00063,T00066,T00067 |
Enzyme Name | UniProtKB | KEGG |
---|
| P22073 | Q03506 | P26205 | P49235 |
---|
Protein name | Beta-glucosidase A | Beta-glucosidase | Cyanogenic beta-glucosidase | Beta-glucosidase, chloroplastic | beta-glucosidasegentiobiasecellobiaseemulsinelaterasearyl-beta-glucosidasebeta-D-glucosidasebeta-glucoside glucohydrolasearbutinaseamygdalinasep-nitrophenyl beta-glucosidaseprimeverosidaseamygdalaselimarasesalicilinasebeta-1,6-glucosidase |
---|
Synonyms | BGAEC 3.2.1.21GentiobiaseCellobiaseBeta-D-glucoside glucohydrolaseAmygdalase | EC 3.2.1.21GentiobiaseCellobiaseBeta-D-glucoside glucohydrolaseAmygdalase | EC 3.2.1.21Linamarase | EC 3.2.1.21GentiobiaseCellobiaseBeta-D-glucoside glucohydrolase |
---|
RefSeq |
|
|
| NP_001105454.1 (Protein) NM_001111984.1 (DNA/RNA sequence)
|
---|
Pfam | PF00232 (Glyco_hydro_1) [Graphical view]
| PF00232 (Glyco_hydro_1) [Graphical view]
| PF00232 (Glyco_hydro_1) [Graphical view]
| PF00232 (Glyco_hydro_1) [Graphical view]
|
---|
CAZy | GH1 (Glycoside Hydrolase Family)
| GH1 (Glycoside Hydrolase Family)
| GH1 (Glycoside Hydrolase Family)
| GH1 (Glycoside Hydrolase Family)
|
---|
KEGG pathways | MAP code | Pathways |
---|
MAP00460 | Cyanoamino acid metabolism | MAP00500 | Starch and sucrose metabolism | MAP00940 | Phenylpropanoid biosynthesis |
UniProtKB:Accession Number | P22073 | Q03506 | P26205 | P49235 |
---|
Entry name | BGLA_PAEPO | BGLA_BACCI | BGLT_TRIRP | BGLC_MAIZE |
---|
Activity | Hydrolysis of terminal, non-reducing beta-D- glucose residues with release of beta-D-glucose. | Hydrolysis of terminal, non-reducing beta-D- glucosyl residues with release of beta-D-glucose. | Hydrolysis of terminal, non-reducing beta-D- glucose residues with release of beta-D-glucose. | Hydrolysis of terminal, non-reducing beta-D- glucose residues with release of beta-D-glucose. |
---|
Subunit | Homooctamer. |
| Homodimer. | Homodimer. |
---|
Subcellular location |
|
|
| Plastid, chloroplast. |
---|
Cofactor |
|
|
|
|
---|
Compound table: links to PDB-related databases & PoSSuM |
---|
| Substrates | Products | intermediates |
---|
KEGG-id | C00963 | C00001 | C00963 | C00221 |
|
---|
Compound | beta-D-Glucoside | H2O | beta-D-Glucoside | beta-D-Glucose | Transition-state in deglycosylation |
---|
Type | carbohydrate | H2O | carbohydrate | carbohydrate |
|
---|
ChEBI |
| 15377
|
| 15903
|
|
---|
PubChem |
| 962 22247451
|
| 64689
|
|
---|
| | | | | | | | | | | | |
---|
1bgaA |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1bgaB |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1bgaC |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1bgaD |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1bggA |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1bggB |  |  |  |  |  |  |  | Unbound | | Unbound | Analogue:GCO | |
---|
1bggC |  |  |  |  |  |  |  | Unbound | | Unbound | Analogue:GCO | |
---|
1bggD |  |  |  |  |  |  |  | Unbound | | Unbound | Analogue:GCO | |
---|
1tr1A |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1tr1B |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1tr1C |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1tr1D |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxA |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxB |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxC |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxD |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxE |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxF |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxG |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxH |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxI |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxJ |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxK |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxL |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxM |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxN |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxO |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1qoxP |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1cbgA |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1e1eA |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1e1eB |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1e1fA |  |  |  |  |  |  |  | Analogue:PSG | | Unbound | Unbound | |
---|
1e1fB |  |  |  |  |  |  |  | Analogue:PSG | | Unbound | Unbound | |
---|
1e4lA |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1e4lB |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1e4nA |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1e4nB |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | |
---|
1e55A |  |  |  |  |  |  |  | Unbound | | Analogue:GLC-DHR | Unbound | |
---|
1e55B |  |  |  |  |  |  |  | Unbound | | Analogue:GLC-DHR | Unbound | |
---|
1e56A |  |  |  |  |  |  |  | Unbound | | Analogue:GLC-HBO | Unbound | |
---|
1e56B |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Tansition-state-analogue:GLC-HBO |
---|
references | [1] |
---|
PubMed ID | 1367638 |
---|
Journal | Enzyme Microb Technol |
---|
Year | 1991 |
---|
Volume | 13 |
---|
Pages | 547-53 |
---|
Authors | Cantarella M, Cantarella L, Alfani F |
---|
Title | Hydrolytic reactions in two-phase systems. Effect of water-immiscible organic solvents on stability and activity of acid phosphatase, beta-glucosidase, and beta-fructofuranosidase. |
---|
[2] |
---|
PubMed ID | 1714449 |
---|
Journal | J Biol Chem |
---|
Year | 1991 |
---|
Volume | 266 |
---|
Pages | 15021-7 |
---|
Authors | Fabbro D, Grabowski GA |
---|
Title | Human acid beta-glucosidase. Use of inhibitory and activating monoclonal antibodies to investigate the enzyme's catalytic mechanism and saposin A and C binding sites. |
---|
[3] |
---|
PubMed ID | 8477701 |
---|
Journal | Eur J Biochem |
---|
Year | 1993 |
---|
Volume | 213 |
---|
Pages | 305-12 |
---|
Authors | Kengen SW, Luesink EJ, Stams AJ, Zehnder AJ |
---|
Title | Purification and characterization of an extremely thermostable beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus. |
---|
[4] |
---|
PubMed ID | 7986074 |
---|
Journal | Arch Biochem Biophys |
---|
Year | 1994 |
---|
Volume | 315 |
---|
Pages | 323-30 |
---|
Authors | Keresztessy Z, Kiss L, Hughes MA |
---|
Title | Investigation of the active site of the cyanogenic beta-D-glucosidase (linamarase) from Manihot esculenta Crantz (cassava). II. Identification of Glu-198 as an active site carboxylate group with acid catalytic function. |
---|
[5] |
---|
PubMed ID | 7944386 |
---|
Journal | Arch Biochem Biophys |
---|
Year | 1994 |
---|
Volume | 314 |
---|
Pages | 142-52 |
---|
Authors | Keresztessy Z, Kiss L, Hughes MA |
---|
Title | Investigation of the active site of the cyanogenic beta-D-glucosidase (linamarase) from Manihot esculenta Crantz (cassava). I. Evidence for an essential carboxylate and a reactive histidine residue in a single catalytic center. |
---|
[6] |
---|
PubMed ID | 8028010 |
---|
Journal | J Mol Biol |
---|
Year | 1994 |
---|
Volume | 240 |
---|
Pages | 267-70 |
---|
Authors | Sanz-Aparicio J, Romero A, Martinez-Ripoll M, Madarro A, Flors A, Polaina J |
---|
Title | Crystallization and preliminary X-ray diffraction analysis of a type I beta-glucosidase encoded by the bgIA gene of Bacillus polymyxa. |
---|
[7] |
---|
PubMed ID | 8588743 |
---|
Journal | Arch Microbiol |
---|
Year | 1995 |
---|
Volume | 164 |
---|
Pages | 414-9 |
---|
Authors | Rath J, Messner R, Kosma P, Altmann F, Marz L, Kubicek CP |
---|
Title | The alpha-D-mannan core of a complex cell-wall heteroglycan of Trichoderma reesei is responsible for beta-glucosidase activation. |
---|
[8] |
---|
PubMed ID | 8519777 |
---|
Journal | Biochemistry |
---|
Year | 1995 |
---|
Volume | 34 |
---|
Pages | 16194-202 |
---|
Authors | Namchuk MN, Withers SG |
---|
Title | Mechanism of Agrobacterium beta-glucosidase: kinetic analysis of the role of noncovalent enzyme/substrate interactions. |
---|
[9] |
---|
PubMed ID | 7578061 |
---|
Journal | Biochemistry |
---|
Year | 1995 |
---|
Volume | 34 |
---|
Pages | 14554-62 |
---|
Authors | Wang Q, Trimbur D, Graham R, Warren RA, Withers SG |
---|
Title | Identification of the acid/base catalyst in Agrobacterium faecalis beta-glucosidase by kinetic analysis of mutants. |
---|
[10] |
---|
Comments | X-ray crystallography (2.15 angstroms). |
---|
Medline ID | 96097401 |
---|
PubMed ID | 8535788 |
---|
Journal | Structure |
---|
Year | 1995 |
---|
Volume | 3 |
---|
Pages | 951-60 |
---|
Authors | Barrett T, Suresh CG, Tolley SP, Dodson EJ, Hughes MA |
---|
Title | The crystal structure of a cyanogenic beta-glucosidase from white clover, a family 1 glycosyl hydrolase. |
---|
Related PDB | 1cbg |
---|
Related UniProtKB | P26205 |
---|
[11] |
---|
PubMed ID | 9636318 |
---|
Journal | Biotechnology (N Y) |
---|
Year | 1996 |
---|
Volume | 14 |
---|
Pages | 88-91 |
---|
Authors | Fischer L, Bromann R, Kengen SW, de Vos WM, Wagner F |
---|
Title | Catalytical potency of beta-glucosidase from the extremophile Pyrococcus furiosus in glucoconjugate synthesis. |
---|
[12] |
---|
PubMed ID | 9214768 |
---|
Journal | Biosci Biotechnol Biochem |
---|
Year | 1997 |
---|
Volume | 61 |
---|
Pages | 1033-5 |
---|
Authors | Nunoura N, Fujita T, Ohdan K, Kirihata M, Yamamoto K, Kumagai H |
---|
Title | Structural analysis of disaccharides synthesized by beta-D-glucosidase of Bifidobacterium breve clb and their assimilation by Bifidobacteria. |
---|
[13] |
---|
PubMed ID | 9657966 |
---|
Journal | Biochem J |
---|
Year | 1998 |
---|
Volume | 333 |
---|
Pages | 275-83 |
---|
Authors | Duroux L, Delmotte FM, Lancelin JM, Keravis G, Jay-Allemand C |
---|
Title | Insight into naphthoquinone metabolism: beta-glucosidase-catalysed hydrolysis of hydrojuglone beta-D-glucopyranoside. |
---|
[14] |
---|
PubMed ID | 9461511 |
---|
Journal | Biochem J |
---|
Year | 1998 |
---|
Volume | 330 |
---|
Pages | 203-9 |
---|
Authors | Lawson SL, Warren RA, Withers SG |
---|
Title | Mechanistic consequences of replacing the active-site nucleophile Glu-358 in Agrobacterium sp. beta-glucosidase with a cysteine residue. |
---|
[15] |
---|
PubMed ID | 9860830 |
---|
Journal | Biochemistry |
---|
Year | 1998 |
---|
Volume | 37 |
---|
Pages | 17170-8 |
---|
Authors | Bauer MW, Kelly RM |
---|
Title | The family 1 beta-glucosidases from Pyrococcus furiosus and Agrobacterium faecalis share a common catalytic mechanism. |
---|
[16] |
---|
PubMed ID | 9630531 |
---|
Journal | Biochim Biophys Acta |
---|
Year | 1998 |
---|
Volume | 1385 |
---|
Pages | 78-88 |
---|
Authors | Watt DK, Ono H, Hayashi K |
---|
Title | Agrobacterium tumefaciens beta-glucosidase is also an effective beta-xylosidase, and has a high transglycosylation activity in the presence of alcohols. |
---|
[17] |
---|
PubMed ID | 9845479 |
---|
Journal | Biol Trace Elem Res |
---|
Year | 1998 |
---|
Volume | 64 |
---|
Pages | 247-58 |
---|
Authors | Sreekala M, Lalitha K |
---|
Title | Selenium-mediated differential response of beta-glucosidase and beta-galactosidase of germinating Trigonella foenum-graecum. |
---|
[18] |
---|
PubMed ID | 9468315 |
---|
Journal | FEBS Lett |
---|
Year | 1998 |
---|
Volume | 421 |
---|
Pages | 243-8 |
---|
Authors | Montero E, Vallmitjana M, Perez-Pons JA, Querol E, Jimenez-Barbero J, Canada FJ |
---|
Title | NMR studies of the conformation of thiocellobiose bound to a beta-glucosidase from Streptomyces sp. |
---|
[19] |
---|
PubMed ID | 9857024 |
---|
Journal | J Biol Chem |
---|
Year | 1998 |
---|
Volume | 273 |
---|
Pages | 34941-8 |
---|
Authors | Hays WS, VanderJagt DJ, Bose B, Serianni AS, Glew RH |
---|
Title | Catalytic mechanism and specificity for hydrolysis and transglycosylation reactions of cytosolic beta-glucosidase from guinea pig liver. |
---|
[20] |
---|
Comments | X-ray crystallography (2.4 angstroms). |
---|
Medline ID | 98139963 |
---|
PubMed ID | 9466926 |
---|
Journal | J Mol Biol |
---|
Year | 1998 |
---|
Volume | 275 |
---|
Pages | 491-502 |
---|
Authors | Sanz-Aparicio J, Hermoso JA, Martinez-Ripoll M, Lequerica JL, Polaina J |
---|
Title | Crystal structure of beta-glucosidase A from Bacillus polymyxa: insights into the catalytic activity in family 1 glycosyl hydrolases. |
---|
Related PDB | 1bga,1bgg,1tr1 |
---|
Related UniProtKB | P22073 |
---|
[21] |
---|
PubMed ID | 9849940 |
---|
Journal | Proteins |
---|
Year | 1998 |
---|
Volume | 33 |
---|
Pages | 567-76 |
---|
Authors | Sanz-Aparicio J, Hermoso JA, Martinez-Ripoll M, Gonzalez B, Lopez-Camacho C, Polaina J |
---|
Title | Structural basis of increased resistance to thermal denaturation induced by single amino acid substitution in the sequence of beta-glucosidase A from Bacillus polymyxa. |
---|
[22] |
---|
PubMed ID | 10467123 |
---|
Journal | Biotechnol Appl Biochem |
---|
Year | 1999 |
---|
Volume | 30 |
---|
Pages | 81-7 |
---|
Authors | Lin J, Pillay B, Singh S |
---|
Title | Purification and biochemical characteristics of beta-D-glucosidase from a thermophilic fungus, Thermomyces lanuginosus-SSBP. |
---|
[23] |
---|
PubMed ID | 10583402 |
---|
Journal | Eur J Biochem |
---|
Year | 1999 |
---|
Volume | 266 |
---|
Pages | 1056-65 |
---|
Authors | Rotrekl V, Nejedla E, Kucera I, Abdallah F, Palme K, Brzobohaty B |
---|
Title | The role of cysteine residues in structure and enzyme activity of a maize beta-glucosidase. |
---|
[24] |
---|
PubMed ID | 10737327 |
---|
Journal | Glycoconj J |
---|
Year | 1999 |
---|
Volume | 16 |
---|
Pages | 415-23 |
---|
Authors | Kono H, Kawano S, Tajima K, Erata T, Takai M |
---|
Title | Structural analyses of new tri- and tetrasaccharides produced from disaccharides by transglycosylation of purified Trichoderma viride beta-glucosidase. |
---|
[25] |
---|
PubMed ID | 10736164 |
---|
Journal | Biochemistry |
---|
Year | 2000 |
---|
Volume | 39 |
---|
Pages | 3656-65 |
---|
Authors | Lebbink JH, Kaper T, Bron P, van der Oost J, de Vos WM |
---|
Title | Improving low-temperature catalysis in the hyperthermostable Pyrococcus furiosus beta-glucosidase CelB by directed evolution. |
---|
[26] |
---|
PubMed ID | 11828410 |
---|
Journal | Chembiochem |
---|
Year | 2000 |
---|
Volume | 1 |
---|
Pages | 177-80 |
---|
Authors | Hansen SU, Plesner IW, Bols M |
---|
Title | Direct NMR-spectroscopic determination of active-enzyme concentration by titration with a labeled inhibitor: determination of the k(cat) value of almond beta-glucosidase. |
---|
[27] |
---|
PubMed ID | 11052758 |
---|
Journal | J Agric Food Chem |
---|
Year | 2000 |
---|
Volume | 48 |
---|
Pages | 4929-36 |
---|
Authors | Decker CH, Visser J, Schreier P |
---|
Title | beta-glucosidases from five black Aspergillus species: study of their physico-chemical and biocatalytic properties. |
---|
[28] |
---|
PubMed ID | 10871610 |
---|
Journal | J Biol Chem |
---|
Year | 2000 |
---|
Volume | 275 |
---|
Pages | 28843-8 |
---|
Authors | Arrizubieta MJ, Polaina J |
---|
Title | Increased thermal resistance and modification of the catalytic properties of a beta-glucosidase by random mutagenesis and in vitro recombination. |
---|
[29] |
---|
Comments | X-ray crystallography (2.7 Angstroms) |
---|
PubMed ID | 10675298 |
---|
Journal | J Struct Biol |
---|
Year | 2000 |
---|
Volume | 129 |
---|
Pages | 69-79 |
---|
Authors | Hakulinen N, Paavilainen S, Korpela T, Rouvinen J |
---|
Title | The Crystal Structure of Beta-Glucosidase from Bacillus Circulans Sp. Alkalophilus: Ability to Form Long Polymeric Assemblies. |
---|
Related PDB | 1qox |
---|
[30] |
---|
Comments | X-ray crystallography (2.0-2.2 Angstroms) |
---|
PubMed ID | 11106394 |
---|
Journal | Proc Nat Acad Sci USA |
---|
Year | 2000 |
---|
Volume | 97 |
---|
Pages | 13555-13560 |
---|
Authors | Czjzek M, Cicek M, Zamboni V, Bevan DR, Henrissat B, Esen A |
---|
Title | The Mechanism of Substrate (Aglycone) Specificity in Beta-Glucosidases is Revealed by Crystal Structures of Mutant Maize Beta-Glucosidase-Dimboa,-Dimboaglc, and-Dhurrin Complexes. |
---|
Related PDB | 1e4l,1e4n,1e55,1e56 |
---|
[31] |
---|
Comments | X-ray crystallography (2.5 Angstroms) |
---|
PubMed ID | 11171077 |
---|
Journal | Biochem J |
---|
Year | 2001 |
---|
Volume | 354 |
---|
Pages | 37-46 |
---|
Authors | Czjzek M, Cicek M, Zamboni V, Burmeister WP, Bevan DR, Henrissat B, Esen A |
---|
Title | Crystal Structure of a Monocotyledon (Maize Zmglu1) Beta-Glucosidase and a Model of its Complex with P-Nitrophenyl Beta-D-Thioglucoside. |
---|
Related PDB | 1e1e,1e1f |
---|
[32] |
---|
PubMed ID | 11115405 |
---|
Journal | Biochem J |
---|
Year | 2001 |
---|
Volume | 353 |
---|
Pages | 117-127 |
---|
Authors | Parry NJ, Beever DE, Owen E, Vandenberghe I, Van Beeumen J, Bhat MK |
---|
Title | Biochemical characterization and mechanism of action of a thermostable beta-glucosidase purified from Thermoascus aurantiacus. |
---|
[33] |
---|
Comments | catalysis, kinetics |
---|
PubMed ID | 11352732 |
---|
Journal | Biochemistry |
---|
Year | 2001 |
---|
Volume | 40 |
---|
Pages | 5975-82 |
---|
Authors | Vallmitjana M, Ferrer-Navarro M, Planell R, Abel M, Ausin C, Querol E, Planas A, Perez-Pons JA |
---|
Title | Mechanism of the family 1 beta-glucosidase from Streptomyces sp: catalytic residues and kinetic studies. |
---|
[34] |
---|
PubMed ID | 11342030 |
---|
Journal | Biochim Biophys Acta |
---|
Year | 2001 |
---|
Volume | 1545 |
---|
Pages | 41-52 |
---|
Authors | Marana SR, Jacobs-Lorena M, Terra WR, Ferreira C |
---|
Title | Amino acid residues involved in substrate binding and catalysis in an insect digestive beta-glycosidase. |
---|
[35] |
---|
PubMed ID | 11520685 |
---|
Journal | Insect Biochem Mol Biol |
---|
Year | 2001 |
---|
Volume | 31 |
---|
Pages | 1065-76 |
---|
Authors | Ferreira AH, Marana SR, Terra WR, Ferreira C |
---|
Title | Purification, molecular cloning, and properties of a beta-glycosidase isolated from midgut lumen of Tenebrio molitor (Coleoptera) larvae. |
---|
[36] |
---|
PubMed ID | 11706179 |
---|
Journal | Plant Physiol |
---|
Year | 2001 |
---|
Volume | 127 |
---|
Pages | 973-85 |
---|
Authors | Zouhar J, Vevodova J, Marek J, Damborsky J, Su XD, Brzobohaty B |
---|
Title | Insights into the functional architecture of the catalytic center of a maize beta-glucosidase Zm-p60.1. |
---|
[37] |
---|
PubMed ID | 12450115 |
---|
Journal | Biosci Biotechnol Biochem |
---|
Year | 2002 |
---|
Volume | 66 |
---|
Pages | 2060-7 |
---|
Authors | Fukuda K, Mori H, Okuyama M, Kimura A, Ozaki H, Yoneyama M, Chiba S |
---|
Title | Identification of essential ionizable groups and evaluation of subsite affinities in the active site of beta-D-glucosidase F1 from a Streptomyces sp. |
---|
[38] |
---|
PubMed ID | 12363364 |
---|
Journal | Biotechnol Prog |
---|
Year | 2002 |
---|
Volume | 18 |
---|
Pages | 1104-8 |
---|
Authors | Maugard T, Enaud E, de la Sayette A, Choisy P, Legoy MD |
---|
Title | Beta-glucosidase-catalyzed hydrolysis of indican from leaves of Polygonum tinctorium. |
---|
comments | This enzyme belongs to the family-1 of glycosidase enzymes, a member family of 4/7 superfamily, which has got the catalytic residues at the C-terminal ends of beta-4 and beta-7 on the (alpha/beta)8 barrel fold. According to the literature [10] & [20], Glu352 and Glu166 (of PDB;1bgaA) act as a nucleophile and an acid-base, respectively. Moreover, the literature suggested that His121 might stabilize the transition-state, and that Tyr296 modulates the pKa and the activity of the nucleophile, Glu352.
|
created | updated |
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2003-12-05 | 2009-03-17 |
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