EzCatDB: S00283
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DB codeS00283
RLCP classification3.105.250000.91 : Transfer
CATH domainDomain 13.40.35.10 : Fructose PermeaseCatalytic domain
E.C.2.7.1.69


Enzyme Name
UniProtKBKEGG

P26380
Protein nameFructose-specific phosphotransferase enzyme IIB componentprotein-Npi-phosphohistidine---sugar phosphotransferase
glucose permease
PTS permease
phosphotransferase, phosphohistidinoprotein-hexose
enzyme IIl4ac
gene glC proteins
gene bglC RNA formation factors
PEP-dependent phosphotransferase enzyme II
PEP-sugar phosphotransferase enzyme II
phosphoenolpyruvate-sugar phosphotransferase enzyme II
phosphohistidinoprotein-hexose phosphotransferase
phosphohistidinoprotein-hexose phosphoribosyltransferase
phosphoprotein factor-hexose phosophotransferase
protein, specific or class, gene bglC
ribonucleic acid formation factor, gene glC
sucrose phosphotransferase system II
protein-Npi-phosphohistidine:sugar N-pros-phosphotransferase
protein-Npi-phosphohistidine:sugar Npi-phosphotransferase
SynonymsEC 2.7.1.69
PTS system fructose-specific EIIB component
EIIB-Fru
p18
RefSeqNP_390584.1 (Protein)
NC_000964.3 (DNA/RNA sequence)
PfamPF03830 (PTSIIB_sorb)
[Graphical view]

KEGG pathways
MAP codePathways
MAP00010Glycolysis / Gluconeogenesis
MAP00051Fructose and mannose metabolism
MAP00052Galactose metabolism
MAP00053Ascorbate and aldarate metabolism
MAP00500Starch and sucrose metabolism
MAP00530Aminosugars metabolism

UniProtKB:Accession NumberP26380
Entry namePTFB_BACSU
ActivityProtein EIIB N(pi)-phospho-L- histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subunit
Subcellular locationCytoplasm.
Cofactor

Compound table: links to PDB-related databases & PoSSuM

SubstratesProducts
KEGG-idC04261C11477C00615C00934
CompoundProtein N(pi)-phospho-L-histidineSugarProtein histidineSugar phosphate
Typearomatic ring (with nitrogen atoms),peptide/protein,phosphate group/phosphate ionpolysaccharidearomatic ring (with nitrogen atoms),peptide/proteinphosphate group/phosphate ion,polysaccharide
ChEBI



PubChem



            
1bleAUnboundUnboundUnboundUnbound

Active-site residues
resource
Swiss-prot;P26380
pdbCatalytic residuesModified residuescomment
           
1bleAHIS 15
HIS 15(phosphorylated)
phosphorylation site

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[2]p.597, Fig.52

references
[1]
PubMed ID9030753
JournalEur J Biochem
Year1997
Volume243
Pages306-14
AuthorsSeip S, Lanz R, Gutknecht R, Flukiger K, Erni B
TitleThe fructose transporter of Bacillus subtilis encoded by the lev operon: backbone assignment and secondary structure of the IIB(Lev) subunit.
[2]
PubMed ID9551099
JournalJ Mol Biol
Year1998
Volume276
Pages591-602
AuthorsSchauder S, Nunn RS, Lanz R, Erni B, Schirmer T
TitleCrystal structure of the IIB subunit of a fructose permease (IIBLev) from Bacillus subtilis.
Related PDB1ble
Related UniProtKBP26380

comments
The same E.C. number (2.7.1.69) appears in S00297, D00527, D00525, S00420, S00046. All of them are enzymes in PTS system.
In the phosphotransferase (PTS) system, a phosphoryl group is transferred from phosphoenolpyruvate (PEP) via the PTS enzymes, EI, HPr, IIA, IIB to the tranported sugar. The enzyme here is IIB subunit of a fructose transporter (IIB-lev).
Phosphoryl transfer between the PTS components is thought to proceed by nucleophilic attack of the active histidine residue on the phospho-histidine of the target protein, resulting in inversion of configuration of the phosphoryl group.

createdupdated
2002-07-282009-03-04


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Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
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