|
Enzyme Name | UniProtKB | KEGG |
---|
| P42790 |
---|
Protein name | Pseudomonalisin | sedolisinPseudomonas sp. pepstatin-insensitive carboxyl proteinasepseudomonapepsinpseudomonalisinsedolysin |
---|
Synonyms | EC 3.4.21.100Pepstatin-insensitive carboxyl proteinasePseudomonapepsin |
---|
MEROPS | S53.001 (Serine)
|
---|
Pfam | PF00082 (Peptidase_S8) PF09286 (Pro-kuma_activ) [Graphical view]
|
---|
UniProtKB:Accession Number | P42790 |
---|
Entry name | PICP_PSESR |
---|
Activity | Hydrolysis of the B chain of insulin at 13- Glu-|-Ala-14, 15-Leu-|-Tyr-16 and 25-Phe-|-Tyr-26 and angiotensin I at 4-Tyr-|-Ile-5. A good synthetic substrate is Lys-Pro-Ile-Glu- Phe-|-Phe(NO(2))-Arg-Leu. |
---|
Subunit |
|
---|
Subcellular location | Periplasm. |
---|
Cofactor | Binds 1 calcium ion per subunit. |
---|
References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
---|
[3] | p.445-446 |
| [4] | p.15609 |
| [5] | p.88-92 |
|
references | [1] |
---|
Comments | active site (mutation analysis) |
---|
Medline ID | 99419069 |
---|
PubMed ID | 10488127 |
---|
Journal | J Biol Chem |
---|
Year | 1999 |
---|
Volume | 274 |
---|
Pages | 27815-22 |
---|
Authors | Hiroshi Oyama, Shin-ichiro Abe, Souko Ushiyama, Saori Takahashi, and Kohei Oda |
---|
Title | Identification of Catalytic Residues of Pepstatin-insensitive Carboxyl Proteinases from Prokaryotes by Site-directed Mutagenesis |
---|
Related PDB | 1ga6 |
---|
Related UniProtKB | P42790 |
---|
[2] |
---|
Comments | X-ray crystallography |
---|
PubMed ID | 11173470 |
---|
Journal | Acta Crystallogr D |
---|
Year | 2001 |
---|
Volume | 57 |
---|
Pages | 239-49 |
---|
Authors | Dauter Z, Li M, Wlodawer A |
---|
Title | Practical experience with the use of halides for phasing macromolecular structures: a powerful tool for structural genomics. |
---|
Related PDB | 1ga1 |
---|
[3] |
---|
Comments | X-ray crystallography (1.0-1.4 Angstroms) |
---|
PubMed ID | 11323721 |
---|
Journal | Nat Struct Biol |
---|
Year | 2001 |
---|
Volume | 8 |
---|
Pages | 442-6 |
---|
Authors | Wlodawer A, Li M, Dauter Z, Gustchina A, Uchida K, Oyama H, Dunn BM, Oda K |
---|
Title | Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes. |
---|
Related PDB | 1ga4,1ga6 |
---|
[4] |
---|
PubMed ID | 11747435 |
---|
Journal | Biochemistry |
---|
Year | 2001 |
---|
Volume | 40 |
---|
Pages | 15602-11 |
---|
Authors | Wlodawer A, Li M, Gustchina A, Dauter Z, Uchida K, Oyama H, Goldfarb NE, Dunn BM, Oda K |
---|
Title | Inhibitor complexes of the Pseudomonas serine-carboxyl proteinase. |
---|
Related PDB | 1kdv,1kdy,1kdz,1ke1,1ke2 |
---|
[5] |
---|
Comments | Review |
---|
PubMed ID | 12673349 |
---|
Journal | Acta Biochim Pol |
---|
Year | 2003 |
---|
Volume | 50 |
---|
Pages | 81-102 |
---|
Authors | Wlodawer A, Li M, Gustchina A, Oyama H, Dunn BM, Oda K |
---|
Title | Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases. |
---|
comments | According to the papers [3], [4] & [5], Ser287 and the acidic residues seem to comprise the catalytic residues, although the detailed catalytic mechanism remains still unknown. However, the catalytic serine residue plays a role as nucleophile, which will attack the carbonyl carbon atom and make a covalent bond with the atom (see [3], [4] & [5]). The paper [3] suggested that Glu80 might be a general base that will abstract the proton of the -OH group of the catalytic serine. Thus, Ser287-Glu80-Asp84 constitute the catalytic triad, whilst Asp170 seems to be a part of oxyanion hole stabilizing the tetrahedral intermediate of the reaction (see [3] & [4]).
|
created | updated |
---|
2002-07-04 | 2011-02-22 |
|
|