|
UniProtKB:Accession Number | P40347 | P11064 | P24666 |
---|
Entry name | PPAL_YEAST | PPAC_BOVIN | PPAC_HUMAN |
---|
Activity | Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,A phosphate monoester + H(2)O = an alcohol + phosphate. | Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,A phosphate monoester + H(2)O = an alcohol + phosphate. | Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,A phosphate monoester + H(2)O = an alcohol + phosphate. |
---|
Subunit |
| Interacts with the SH3 domain of SPTAN1 (By similarity). | Isoform 1 interacts with the SH3 domain of SPTAN1. There is no interaction observed for isoforms 2 or 3. |
---|
Subcellular location | Cytoplasm. | Cytoplasm. | Cytoplasm. |
---|
Cofactor |
|
|
|
---|
References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
---|
[1] | p.652, p.654 |
| [5] | p.11094 |
| [6] | p.11101-11104 |
| [7] | p.576 |
| [8] | p.25948-25950 |
| [10] | Fig.3 |
| [11] | p.5432-5433 |
| [12] | p.20-22 |
| [13] | p.284-286 |
| [14] | p.7933-7935 |
| [15] | Fig.7 | 2 | [18] | p.1240 |
| [20] | p.13580-13581, Fig.3 | 3 |
references | [1] |
---|
PubMed ID | 8319676 |
---|
Journal | Eur J Biochem |
---|
Year | 1993 |
---|
Volume | 214 |
---|
Pages | 647-57 |
---|
Authors | Cirri P, Chiarugi P, Camici G, Manao G, Raugei G, Cappugi G, Ramponi G |
---|
Title | The role of Cys12, Cys17 and Arg18 in the catalytic mechanism of low-M(r) cytosolic phosphotyrosine protein phosphatase. |
---|
[2] |
---|
PubMed ID | 8110762 |
---|
Journal | Biochemistry |
---|
Year | 1994 |
---|
Volume | 33 |
---|
Pages | 1278-86 |
---|
Authors | Davis JP, Zhou MM, Van Etten RL |
---|
Title | Spectroscopic and kinetic studies of the histidine residues of bovine low-molecular-weight phosphotyrosyl protein phosphatase. |
---|
[3] |
---|
PubMed ID | 8135752 |
---|
Journal | Biochem J |
---|
Year | 1994 |
---|
Volume | 298 |
---|
Pages | 427-33 |
---|
Authors | Chiarugi P, Cirri P, Camici G, Manao G, Fiaschi T, Raugei G, Cappugi G, Ramponi G |
---|
Title | The role of His66 and His72 in the reaction mechanism of bovine liver low-M(r) phosphotyrosine protein phosphatase. |
---|
[4] |
---|
Comments | NMR |
---|
Medline ID | 94227053 |
---|
PubMed ID | 8172896 |
---|
Journal | Biochemistry |
---|
Year | 1994 |
---|
Volume | 33 |
---|
Pages | 5221-9 |
---|
Authors | Zhou MM, Logan TM, Theriault Y, Van Etten RL, Fesik SW |
---|
Title | Backbone 1H, 13C, and 15N assignments and secondary structure of bovine low molecular weight phosphotyrosyl protein phosphatase. |
---|
Related UniProtKB | P11064 |
---|
[5] |
---|
Comments | NMR |
---|
PubMed ID | 7727361 |
---|
Journal | Biochemistry |
---|
Year | 1994 |
---|
Volume | 33 |
---|
Pages | 11087-96 |
---|
Authors | Logan TM, Zhou MM, Nettesheim DG, Meadows RP, Van Etten RL, Fesik SW |
---|
Title | Solution structure of a low molecular weight protein tyrosine phosphatase. |
---|
Related PDB | 1bvh |
---|
[6] |
---|
Comments | X-ray crystallography (2.2 Angstroms) |
---|
PubMed ID | 7537084 |
---|
Journal | Biochemistry |
---|
Year | 1994 |
---|
Volume | 33 |
---|
Pages | 11097-105 |
---|
Authors | Zhang M, Van Etten RL, Stauffacher CV |
---|
Title | Crystal structure of bovine heart phosphotyrosyl phosphatase at 2.2-A resolution. |
---|
Related PDB | 1pnt |
---|
[7] |
---|
Comments | X-ray crystallography |
---|
Medline ID | 94329182 |
---|
PubMed ID | 8052313 |
---|
Journal | Nature |
---|
Year | 1994 |
---|
Volume | 370 |
---|
Pages | 575-8 |
---|
Authors | Su XD, Taddei N, Stefani M, Ramponi G, Nordlund P |
---|
Title | The crystal structure of a low-molecular-weight phosphotyrosine protein phosphatase. |
---|
Related PDB | 1phr |
---|
Related UniProtKB | P11064 |
---|
[8] |
---|
PubMed ID | 7929301 |
---|
Journal | J Biol Chem |
---|
Year | 1994 |
---|
Volume | 269 |
---|
Pages | 25947-50 |
---|
Authors | Zhang Z, Harms E, Van Etten RL |
---|
Title | Asp129 of low molecular weight protein tyrosine phosphatase is involved in leaving group protonation. |
---|
[9] |
---|
PubMed ID | 8132604 |
---|
Journal | J Biol Chem |
---|
Year | 1994 |
---|
Volume | 269 |
---|
Pages | 8734-40 |
---|
Authors | Davis JP, Zhou MM, Van Etten RL |
---|
Title | Kinetic and site-directed mutagenesis studies of the cysteine residues of bovine low molecular weight phosphotyrosyl protein phosphatase. |
---|
[10] |
---|
PubMed ID | 7577995 |
---|
Journal | Biochemistry |
---|
Year | 1995 |
---|
Volume | 34 |
---|
Pages | 13982-7 |
---|
Authors | Hengge AC, Sowa GA, Wu L, Zhang ZY |
---|
Title | Nature of the transition state of the protein-tyrosine phosphatase-catalyzed reaction. |
---|
[11] |
---|
PubMed ID | 8611532 |
---|
Journal | Biochemistry |
---|
Year | 1996 |
---|
Volume | 35 |
---|
Pages | 5426-34 |
---|
Authors | Wu L, Zhang ZY |
---|
Title | Probing the function of Asp128 in the lower molecular weight protein-tyrosine phosphatase-catalyzed reaction. A pre-steady-state and steady-state kinetic investigation. |
---|
[12] |
---|
Comments | X-ray crystallography (2.2 Angstroms) (with the transition state analog vanadate) |
---|
Medline ID | 97146457 |
---|
PubMed ID | 8993313 |
---|
Journal | Biochemistry |
---|
Year | 1997 |
---|
Volume | 36 |
---|
Pages | 15-23 |
---|
Authors | Zhang M, Zhou M, Van Etten RL, Stauffacher CV |
---|
Title | Crystal structure of bovine low molecular weight phosphotyrosyl phosphatase complexed with the transition state analog vanadate. |
---|
Related UniProtKB | P11064 |
---|
[13] |
---|
Comments | Review |
---|
PubMed ID | 9147129 |
---|
Journal | Int J Biochem Cell Biol |
---|
Year | 1997 |
---|
Volume | 29 |
---|
Pages | 279-92 |
---|
Authors | Ramponi G, Stefani M |
---|
Title | Structural, catalytic, and functional properties of low M(r), phosphotyrosine protein phosphatases. Evidence of a long evolutionary history. |
---|
[14] |
---|
PubMed ID | 9201938 |
---|
Journal | Biochemistry |
---|
Year | 1997 |
---|
Volume | 36 |
---|
Pages | 7928-36 |
---|
Authors | Hengge AC, Zhao Y, Wu L, Zhang ZY |
---|
Title | Examination of the transition state of the low-molecular mass small tyrosine phosphatase 1. Comparisons with other protein phosphatases. |
---|
[15] |
---|
PubMed ID | 9488671 |
---|
Journal | J Biol Chem |
---|
Year | 1998 |
---|
Volume | 273 |
---|
Pages | 5484-92 |
---|
Authors | Zhao Y, Wu L, Noh SJ, Guan KL, Zhang ZY |
---|
Title | Altering the nucleophile specificity of a protein-tyrosine phosphatase-catalyzed reaction. Probing the function of the invariant glutamine residues. |
---|
[16] |
---|
Comments | X-ray crystallography (2.2 Angstroms) |
---|
Medline ID | 98371007 |
---|
PubMed ID | 9705307 |
---|
Journal | J Biol Chem |
---|
Year | 1998 |
---|
Volume | 273 |
---|
Pages | 21714-20 |
---|
Authors | Zhang M, Stauffacher CV, Lin D, Van Etten RL |
---|
Title | Crystal structure of a human low molecular weight phosphotyrosyl phosphatase. Implications for substrate specificity. |
---|
Related PDB | 5pnt |
---|
Related UniProtKB | P24666 |
---|
[17] |
---|
Comments | X-ray crystallography (S19A mutant) |
---|
PubMed ID | 10512620 |
---|
Journal | Biochemistry |
---|
Year | 1999 |
---|
Volume | 38 |
---|
Pages | 11651-8 |
---|
Authors | Tabernero L, Evans BN, Tishmack PA, Van Etten RL, Stauffacher CV |
---|
Title | The structure of the bovine protein tyrosine phosphatase dimer reveals a potential self-regulation mechanism. |
---|
Related PDB | 1c0e |
---|
[18] |
---|
Comments | X-ray crystallography (with adenine which acts as an activator.) |
---|
PubMed ID | 10684601 |
---|
Journal | Biochemistry |
---|
Year | 2000 |
---|
Volume | 39 |
---|
Pages | 1234-42 |
---|
Authors | Wang S, Stauffacher CV, Van Etten RL |
---|
Title | Structural and mechanistic basis for the activation of a low-molecular weight protein tyrosine phosphatase by adenine. |
---|
Related PDB | 1d2a |
---|
[19] |
---|
Comments | X-ray crystallography (wild type;2.2 Angstroms, mutant;1.7 Angstroms) |
---|
PubMed ID | 10684639 |
---|
Journal | Biochemistry |
---|
Year | 2000 |
---|
Volume | 39 |
---|
Pages | 1903-14 |
---|
Authors | Wang S, Tabernero L, Zhang M, Harms E, Van Etten RL, Stauffacher CV |
---|
Title | Crystal structures of a low-molecular weight protein tyrosine phosphatase from Saccharomyces cerevisiae and its complex with the substrate p-nitrophenyl phosphate. |
---|
Related PDB | 1d1p,1d1q |
---|
[20] |
---|
Comments | X-ray crystallography (of the other but similar protein) |
---|
PubMed ID | 11698660 |
---|
Journal | Proc Natl Acad Sci U S A |
---|
Year | 2001 |
---|
Volume | 98 |
---|
Pages | 13577-82 |
---|
Authors | Bennett MS, Guan Z, Laurberg M, Su XD |
---|
Title | Bacillus subtilis arsenate reductase is structurally and functionally similar to low molecular weight protein tyrosine phosphatases. |
---|
comments | This enzyme hydrolyzes the tyrosine phosphate. The paper [6] described the catalytic role of the Arg18, Cys12 and Asp129 (PDB; 1pnt). According to the paper, Arg18 can serve in the orientation and stabilization of the substrate phosphate, whilst Cys12 is the nucleophilic residue, whose thiolate anion will attack the phosphorous atom of substrate phosphate, leading to the phosphoenzyme. During the phosphorylation event, Asp129 can protonate to the leaving group [6]. After the formation of a phosphoenzyme intermediate, the dephosphorylation reaction, which is the rate-limiting step, would occur by attack of water molecule on the phosphorylated cysteine with the subsequent release of the inorganic phosphate [6]. Here, the paper [6] mentioned that the water attacks the intermediate without the assistance of a general base, whilst the literature [7] suggested that Asp129 can serve as ageneral base for the water nucleophile in the dephosphorylation event. The papers on crystal structures ([6], [7] & [12]) suggested that the phosphorylation reaction proceeds in an in-line associative mechanism (SN2-like reaction), whilst other papers on the biochemical data ([10], [11] & [14]) indicated that this reaction is rather a dissociative one (SN1-like reaction).
|
created | updated |
---|
2002-08-01 | 2009-04-03 |
|
|