EzCatDB: S00345
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DB codeS00345
RLCP classification1.12.30000.10 : Hydrolysis
CATH domainDomain 13.40.50.1820 : Rossmann foldCatalytic domain
E.C.3.1.1.1
CSA1auo

CATH domainRelated DB codes (homologues)
3.40.50.1820 : Rossmann foldS00544,S00344,S00517,S00525,S00526,S00720,S00723,S00724,S00725,S00919,S00057,S00374,S00347,S00348,S00346,S00350,S00352,S00353,S00355,S00356,S00358,D00189,D00210,D00539,T00253

Enzyme Name
UniProtKBKEGG

Q53547
Protein nameCarboxylesterase 2carboxylesterase
ali-esterase
B-esterase
monobutyrase
cocaine esterase
procaine esterase
methylbutyrase
vitamin A esterase
butyryl esterase
carboxyesterase
carboxylate esterase
carboxylic esterase
methylbutyrate esterase
triacetin esterase
carboxyl ester hydrolase
butyrate esterase
methylbutyrase
alpha-carboxylesterase
propionyl esterase
nonspecific carboxylesterase
esterase D
esterase B
esterase A
serine esterase
carboxylic acid esterase
cocaine esterase
SynonymsEC 3.1.1.1
Esterase II
PfamPF02230 (Abhydrolase_2)
[Graphical view]

KEGG pathways
MAP codePathways
MAP00960Alkaloid biosynthesis II
MAP00983Drug metabolism - other enzymes

UniProtKB:Accession NumberQ53547
Entry nameEST2_PSEFL
ActivityA carboxylic ester + H(2)O = an alcohol + a carboxylate.
SubunitHomodimer.
Subcellular location
Cofactor

Compound table: links to PDB-related databases & PoSSuM

SubstratesProductsintermediates
KEGG-idC02391C00001C00069C00060I00123I00085I00086
CompoundCarboxylic esterH2OAlcoholCarboxylatePeptidyl-Ser-tetrahedral intermediate (with previous carboxylic-ester)Acyl-enzyme(Peptidyl-Ser-acyl group)Peptidyl-Ser-tetrahedral-intermediate
TypecarbohydrateH2Ocarbohydratecarboxyl group


ChEBI
15377





PubChem
962
22247451





               
1auoAUnbound UnboundUnboundUnboundUnboundUnbound
1auoBUnbound UnboundUnboundUnboundUnboundUnbound
1aurAUnbound UnboundUnboundUnboundUnboundAnalogue:PMS
1aurBUnbound UnboundUnboundUnboundUnboundAnalogue:PMS

Active-site residues
resource
Swiss-prot, PDB & literature
pdbCatalytic residuesMain-chain involved in catalysis
          
1auoASER 114;ASP 168;HIS 199
LEU 23;GLN 115
1auoBSER 114;ASP 168;HIS 199
LEU 23;GLN 115
1aurASER 114;ASP 168;HIS 199
LEU 23;GLN 115
1aurBSER 114;ASP 168;HIS 199
LEU 23;GLN 115

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[1]p.1574-1576
[2]p.762-763
[3]p.12300-12301

references
[1]
CommentsX-ray crystallography (1.8 Angstroms)
PubMed ID9438866
JournalStructure
Year1997
Volume5
Pages1571-84
AuthorsKim KK, Song HK, Shin DH, Hwang KY, Choe S, Yoo OJ, Suh SW
TitleCrystal structure of carboxylesterase from Pseudomonas fluorescens, an alpha/beta hydrolase with broad substrate specificity.
Related PDB1auo,1aur
Related UniProtKBQ53547
[2]
CommentsX-ray crystallography (2.6 Angstroms)
PubMed ID11061974
JournalJ Mol Biol
Year2000
Volume303
Pages761-71
AuthorsDe Simone G, Galdiero S, Manco G, Lang D, Rossi M, Pedone C
TitleA snapshot of a transition state analogue of a novel thermophilic esterase belonging to the subfamily of mammalian hormone-sensitive lipase.
Related PDB1evq
[3]
CommentsHomologous enzyme
PubMed ID12369817
JournalBiochemistry
Year2002
Volume41
Pages12297-307
AuthorsTurner JM, Larsen NA, Basran A, Barbas CF 3rd, Bruce NC, Wilson IA, Lerner RA
TitleBiochemical characterization and structural analysis of a highly proficient cocaine esterase.


createdupdated
2002-07-112012-10-22


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