EzCatDB: S00381
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DB codeS00381
CATH domainDomain 13.40.50.7700 : Rossmann foldCatalytic domain
E.C.4.1.1.21


Enzyme Name
UniProtKBKEGG

P0AG18
Protein nameN5-carboxyaminoimidazole ribonucleotide mutasephosphoribosylaminoimidazole carboxylase
5-phosphoribosyl-5-aminoimidazole carboxylase
5-amino-1-ribosylimidazole 5-phosphate carboxylase
AIR carboxylase
1-(5-phosphoribosyl)-5-amino-4-imidazolecarboxylate carboxy-lyase
ADE2
class II PurE
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate carboxy-lyase
SynonymsN5-CAIR mutase
EC 5.4.99.18
5-(carboxyamino)imidazole ribonucleotide mutase
RefSeqNP_415056.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_488812.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PfamPF00731 (AIRC)
[Graphical view]

KEGG pathways
MAP codePathways
MAP00230Purine metabolism

UniProtKB:Accession NumberP0AG18
Entry namePUR6_ECOLI
Activity5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2).
SubunitHomooctamer.
Subcellular location
Cofactor

Compound table: links to PDB-related databases & PoSSuM

SubstratesProducts
KEGG-idC04751C00011C03373
Compound1-(5-Phospho-D-ribosyl)-5-amino-4-imidazolecarboxylateCO21-(5-Phospho-D-ribosyl)-5-aminoimidazole
Typeamine group,carboxyl group,nucleotideothersamine group,nucleotide
ChEBI28413
16526

PubChem165388
280
161500
           
1d7aAUnboundUnboundBound:AIR
1d7aBUnboundUnboundBound:AIR
1d7aCUnboundUnboundBound:AIR
1d7aDUnboundUnboundBound:AIR
1d7aLUnboundUnboundUnbound
1d7aMUnboundUnboundUnbound
1d7aNUnboundUnboundUnbound
1d7aOUnboundUnboundUnbound
1qczAUnboundUnboundUnbound

Active-site residues
pdb
        
1d7aA
1d7aB
1d7aC
1d7aD
1d7aL
1d7aM
1d7aN
1d7aO
1qczA

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[7]Fig.43
[8]p.1399-1402

references
[1]
PubMed ID1797375
JournalCarbohydr Res
Year1991
Volume216
Pages109-18
AuthorsEwing DF, Holy A, Votruba I, Humble RW, Mackenzie G, Hewedi F, Shaw G
TitleSynthesis of 4- and 5-amino-1-(2-deoxy-D-erythro-pentofuranosyl)imidazole nucleosides by chemical and biotransformation methods.
[2]
CommentsCHARACTERIZATION, AND SEQUENCE OF 1-10.
Medline ID92287929
PubMed ID1534690
JournalBiochemistry
Year1992
Volume31
Pages5022-32
AuthorsMeyer E, Leonard NJ, Bhat B, Stubbe J, Smith JM
TitlePurification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.
Related UniProtKBP0AG18
[3]
PubMed ID7918411
JournalBiochemistry
Year1994
Volume33
Pages11927-34
AuthorsFirestine SM, Poon SW, Mueller EJ, Stubbe J, Davisson VJ
TitleReactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms.
[4]
PubMed ID8117684
JournalBiochemistry
Year1994
Volume33
Pages2269-78
AuthorsMueller EJ, Meyer E, Rudolph J, Davisson VJ, Stubbe J
TitleN5-carboxyaminoimidazole ribonucleotide: evidence for a new intermediate and two new enzymatic activities in the de novo purine biosynthetic pathway of Escherichia coli.
[5]
PubMed ID9341908
JournalJ Med Chem
Year1997
Volume40
Pages3336-45
AuthorsGroziak MP, Huan ZW, Ding H, Meng Z, Stevens WC, Robinson PD
TitleEffect of a chemical modification on the hydrated adenosine intermediate produced by adenosine deaminase and a model reaction for a potential mechanism of action of 5-aminoimidazole ribonucleotide carboxylase.
[6]
PubMed ID9500840
JournalArch Biochem Biophys
Year1998
Volume351
Pages123-34
AuthorsFirestine SM, Misialek S, Toffaletti DL, Klem TJ, Perfect JR, Davisson VJ
TitleBiochemical role of the Cryptococcus neoformans ADE2 protein in fungal de novo purine biosynthesis.
[7]
PubMed ID10074353
JournalBiochemistry
Year1999
Volume38
Pages3012-8
AuthorsMeyer E, Kappock TJ, Osuji C, Stubbe J
TitleEvidence for the direct transfer of the carboxylate of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to generate 4-carboxy-5-aminoimidazole ribonucleotide catalyzed by Escherichia coli PurE, an N5-CAIR mutase.
[8]
CommentsX-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
Medline ID20045189
PubMed ID10574791
JournalStructure Fold Des
Year1999
Volume7
Pages1395-406
AuthorsMathews II, Kappock TJ, Stubbe J, Ealick SE
TitleCrystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway.
Related PDB1d7a,1qcz
Related UniProtKBP0AG18


createdupdated
2004-03-312009-02-26


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