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Enzyme Name | UniProtKB | KEGG |
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| P16083 |
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Protein name | Ribosyldihydronicotinamide dehydrogenase {quinone} | ribosyldihydronicotinamide dehydrogenase (quinone)NRH:quinone oxidoreductase 2NQO2NQO2NAD(P)H:quinone oxidoreductase-2 (misleading)QR2quinone reductase 2N-ribosyldihydronicotinamide dehydrogenase (quinone)NAD(P)H:quinone oxidoreductase2 (misleading) |
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Synonyms | EC 1.10.99.2NRH dehydrogenase {quinone} 2NRH:quinone oxidoreductase 2Quinone reductase 2QR2 |
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RefSeq | NP_000895.2 (Protein) NM_000904.3 (DNA/RNA sequence)
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Pfam | PF02525 (Flavodoxin_2) [Graphical view]
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UniProtKB:Accession Number | P16083 |
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Entry name | NQO2_HUMAN |
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Activity | 1-(beta-D-ribofuranosyl)-1,4- dihydronicotinamide + a quinone = 1-(beta-D- ribofuranosyl)nicotinamide + a hydroquinone. |
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Subunit | Homodimer. |
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Subcellular location | Cytoplasm. |
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Cofactor | Binds 1 zinc ion per subunit.,FAD. |
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Compound table: links to PDB-related databases & PoSSuM |
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| Cofactors | Substrates | Products |
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KEGG-id | C00016 | C00038 | C00005 | C00004 | C00080 | C01628 | C00006 | C00003 | C05850 |
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Compound | FAD | Zinc | NADPH | NADH | H+ | Vitamin K | NADP+ | NAD+ | Reduced Vitamin K |
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Type | amide group,amine group,aromatic ring (only carbon atom),aromatic ring (with nitrogen atoms),carbohydrate,nucleotide | heavy metal | amide group,amine group,nucleotide | amide group,amine group,nucleotide | others | aromatic ring (only carbon atom),carbohydrate,lipid | amide group,amine group,nucleotide | amide group,amine group,nucleotide | aromatic ring (only carbon atom),carbohydrate,lipid |
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ChEBI | 16238
| 29105
| 16474
| 16908
| 15378
|
| 18009
| 15846
|
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PubChem | 643975
| 32051
| 5884
| 439153
| 1038
|
| 5886
| 5893
|
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| | | | | | | | | | | | | | | | |
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1qr2A |  |  |  |  |  |  |  | Bound:FAD | Bound:_ZN | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound |
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1qr2B |  |  |  |  |  |  |  | Bound:FAD | Bound:_ZN | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound |
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2qr2A |  |  |  |  |  |  |  | Bound:FAD | Bound:_ZN | Unbound | Unbound | | Bound:VK3 | Unbound | Unbound | Unbound |
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2qr2B |  |  |  |  |  |  |  | Bound:FAD | Bound:_ZN | Unbound | Unbound | | Bound:VK3 | Unbound | Unbound | Unbound |
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References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
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[3] | Fig.8, p.8849-8850 |
| [6] | p.612-614 |
| [7] | p.9985-9986 |
| [10] | p.278-279 |
| [13] | Fig.1, Fig.4, p.130-131 |
| [14] | p.244 |
| [15] | p.664-665 |
| [19] | Fig.8 |
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references | [1] |
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PubMed ID | 1510975 |
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Journal | Biochemistry |
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Year | 1992 |
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Volume | 31 |
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Pages | 7879-85 |
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Authors | Siegel D, Beall H, Senekowitsch C, Kasai M, Arai H, Gibson NW, Ross D |
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Title | Bioreductive activation of mitomycin C by DT-diaphorase. |
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[2] |
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PubMed ID | 7530954 |
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Journal | Biochem Pharmacol |
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Year | 1995 |
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Volume | 49 |
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Pages | 127-40 |
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Authors | Cadenas E |
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Title | Antioxidant and prooxidant functions of DT-diaphorase in quinone metabolism. |
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[3] |
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Comments | X-ray crystallography |
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PubMed ID | 7568029 |
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Journal | Proc Natl Acad Sci U S A |
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Year | 1995 |
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Volume | 92 |
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Pages | 8846-50 |
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Authors | Li R, Bianchet MA, Talalay P, Amzel LM |
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Title | The three-dimensional structure of NAD(P)H:quinone reductase, a flavoprotein involved in cancer chemoprotection and chemotherapy: mechanism of the two-electron reduction. |
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Related PDB | 1qrd |
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[4] |
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PubMed ID | 8999809 |
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Journal | J Biol Chem |
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Year | 1997 |
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Volume | 272 |
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Pages | 1437-9 |
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Authors | Chen S, Knox R, Wu K, Deng PS, Zhou D, Bianchet MA, Amzel LM |
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Title | Molecular basis of the catalytic differences among DT-diaphorase of human, rat, and mouse. |
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[5] |
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PubMed ID | 9050836 |
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Journal | Proc Natl Acad Sci U S A |
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Year | 1997 |
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Volume | 94 |
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Pages | 1669-74 |
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Authors | Zhao Q, Yang XL, Holtzclaw WD, Talalay P |
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Title | Unexpected genetic and structural relationships of a long-forgotten flavoenzyme to NAD(P)H:quinone reductase (DT-diaphorase). |
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[6] |
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PubMed ID | 10917652 |
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Journal | Biochem Soc Trans |
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Year | 1999 |
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Volume | 27 |
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Pages | 610-5 |
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Authors | Bianchet MA, Foster C, Faig M, Talalay P, Amzel LM |
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Title | Structure and mechanism of cytosolic quinone reductases. |
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[7] |
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Comments | X-ray crystallography |
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PubMed ID | 10433694 |
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Journal | Biochemistry |
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Year | 1999 |
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Volume | 38 |
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Pages | 9881-6 |
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Authors | Foster CE, Bianchet MA, Talalay P, Zhao Q, Amzel LM |
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Title | Crystal structure of human quinone reductase type 2, a metalloflavoprotein. |
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Related PDB | 1qr2,2qr2 |
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[8] |
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Comments | X-ray crystallography |
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PubMed ID | 10543876 |
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Journal | J Med Chem |
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Year | 1999 |
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Volume | 42 |
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Pages | 4325-30 |
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Authors | Skelly JV, Sanderson MR, Suter DA, Baumann U, Read MA, Gregory DS, Bennett M, Hobbs SM, Neidle S |
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Title | Crystal structure of human DT-diaphorase: a model for interaction with the cytotoxic prodrug 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954). |
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Related PDB | 1qbg |
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[9] |
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PubMed ID | 10419545 |
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Journal | Mol Pharmacol |
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Year | 1999 |
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Volume | 56 |
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Pages | 272-8 |
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Authors | Chen S, Wu K, Zhang D, Sherman M, Knox R, Yang CS |
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Title | Molecular characterization of binding of substrates and inhibitors to DT-diaphorase: combined approach involving site-directed mutagenesis, inhibitor-binding analysis, and computer modeling. |
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[10] |
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PubMed ID | 11035256 |
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Journal | Free Radic Biol Med |
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Year | 2000 |
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Volume | 29 |
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Pages | 276-84 |
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Authors | Chen S, Wu K, Knox R |
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Title | Structure-function studies of DT-diaphorase (NQO1) and NRH: quinone oxidoreductase (NQO2). |
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[11] |
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PubMed ID | 10877993 |
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Journal | Front Biosci |
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Year | 2000 |
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Volume | 5 |
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Pages | D639-48 |
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Authors | Beall HD, Winski SI |
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Title | Mechanisms of action of quinone-containing alkylating agents. I: NQO1-directed drug development. |
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[12] |
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Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). |
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Medline ID | 20202608 |
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PubMed ID | 10706635 |
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Journal | Proc Natl Acad Sci U S A |
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Year | 2000 |
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Volume | 97 |
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Pages | 3177-82 |
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Authors | Faig M, Bianchet MA, Talalay P, Chen S, Winski S, Ross D, Amzel LM |
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Title | Structures of recombinant human and mouse NAD(P)H:quinone oxidoreductases: species comparison and structural changes with substrate binding and release. |
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Related PDB | 1d4a,1dxo,1dxq |
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Related UniProtKB | P15559 |
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[13] |
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PubMed ID | 10694883 |
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Journal | Trends Biochem Sci |
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Year | 2000 |
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Volume | 25 |
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Pages | 126-32 |
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Authors | Fraaije MW, Mattevi A |
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Title | Flavoenzymes: diverse catalysts with recurrent features. |
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[14] |
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PubMed ID | 11035252 |
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Journal | Free Radic Biol Med |
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Year | 2000 |
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Volume | 29 |
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Pages | 241-5 |
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Authors | Foster CE, Bianchet MA, Talalay P, Faig M, Amzel LM |
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Title | Structures of mammalian cytosolic quinone reductases. |
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[15] |
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Comments | X-ray crystallography |
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PubMed ID | 11587640 |
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Journal | Structure (Camb) |
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Year | 2001 |
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Volume | 9 |
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Pages | 659-67 |
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Authors | Faig M, Bianchet MA, Winski S, Hargreaves R, Moody CJ, Hudnott AR, Ross D, Amzel LM |
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Title | Structure-based development of anticancer drugs: complexes of NAD(P)H:quinone oxidoreductase 1 with chemotherapeutic quinones. |
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Related PDB | 1gg5,1h66,1h69 |
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[16] |
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Comments | X-ray crystallography |
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PubMed ID | 11735396 |
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Journal | Biochemistry |
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Year | 2001 |
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Volume | 40 |
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Pages | 15135-42 |
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Authors | Winski SL, Faig M, Bianchet MA, Siegel D, Swann E, Fung K, Duncan MW, Moody CJ, Amzel LM, Ross D |
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Title | Characterization of a mechanism-based inhibitor of NAD(P)H:quinone oxidoreductase 1 by biochemical, X-ray crystallographic, and mass spectrometric approaches. |
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Related PDB | 1kbo,1kbq |
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[17] |
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PubMed ID | 11340659 |
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Journal | Proteins |
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Year | 2001 |
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Volume | 43 |
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Pages | 420-32 |
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Authors | Cavelier G, Amzel LM |
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Title | Mechanism of NAD(P)H:quinone reductase: Ab initio studies of reduced flavin. |
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[18] |
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PubMed ID | 12147263 |
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Journal | Arch Biochem Biophys |
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Year | 2002 |
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Volume | 404 |
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Pages | 254-62 |
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Authors | Anusevicius Z, Sarlauskas J, Cenas N |
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Title | Two-electron reduction of quinones by rat liver NAD(P)H:quinone oxidoreductase: quantitative structure-activity relationships. |
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[19] |
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PubMed ID | 15078100 |
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Journal | Biochemistry |
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Year | 2004 |
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Volume | 43 |
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Pages | 4538-47 |
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Authors | Kwiek JJ, Haystead TA, Rudolph J |
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Title | Kinetic mechanism of quinone oxidoreductase 2 and its inhibition by the antimalarial quinolines. |
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comments | This enzyme has a similar catalytic mechanism to that of QR1 (S00343 in EzCatDB), alghough it is slightly different. This enzyme involves a metal ion in catalysis (see [7]).
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created | updated |
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2004-10-26 | 2009-02-26 |
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