EzCatDB: T00082
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DB codeT00082
CATH domainDomain 13.40.50.20 : Rossmann fold
Domain 23.30.470.20 : D-amino Acid Aminotransferase; Chain A, domain 1Catalytic domain
Domain 33.30.1490.20 : Dna Ligase; domain 1
E.C.4.1.1.21

CATH domainRelated DB codes (homologues)
3.30.1490.20 : Dna Ligase; domain 1M00035,M00037,T00107,T00108
3.30.470.20 : D-amino Acid Aminotransferase; Chain A, domain 1D00298,M00035,M00037,M00051,T00107,T00108
3.40.50.20 : Rossmann foldM00037,T00107,T00108

Enzyme Name
UniProtKBKEGG

P09029
Protein nameN5-carboxyaminoimidazole ribonucleotide synthasephosphoribosylaminoimidazole carboxylase
5-phosphoribosyl-5-aminoimidazole carboxylase
5-amino-1-ribosylimidazole 5-phosphate carboxylase
AIR carboxylase
1-(5-phosphoribosyl)-5-amino-4-imidazolecarboxylate carboxy-lyase
ADE2
class II PurE
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate carboxy-lyase
SynonymsN5-CAIR synthase
EC 6.3.4.18
5-(carboxyamino)imidazole ribonucleotide synthetase
RefSeqNP_415055.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_488811.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PfamPF02222 (ATP-grasp)
[Graphical view]

KEGG pathways
MAP codePathways
MAP00230Purine metabolism

UniProtKB:Accession NumberP09029
Entry namePURK_ECOLI
Activity5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2).
SubunitHomodimer.
Subcellular location
Cofactor

Compound table: links to PDB-related databases & PoSSuM

CofactorsSubstratesProducts
KEGG-idC00305C03373C00011C00002C00001L00008C00008C00009
CompoundMagnesium1-(5-Phospho-D-ribosyl)-5-aminoimidazoleCO2ATPH2O1-(5-Phospho-D-ribosyl)-5-carboxyamino-imidazoleADPOrthophosphate
Typedivalent metal (Ca2+, Mg2+)amine group,nucleotideothersamine group,nucleotideH2Oamide group,carboxyl group,nucleotideamine group,nucleotidephosphate group/phosphate ion
ChEBI18420

16526
15422
15377
48000
16761
26078
PubChem888
161500
280
5957
962
22247451
23657851
6022
22486802
1004
                
1b6rA01UnboundUnboundUnboundUnbound UnboundUnboundUnbound
1b6sA01UnboundUnboundUnboundUnbound UnboundUnboundUnbound
1b6sB01UnboundUnboundUnboundUnbound UnboundUnboundUnbound
1b6sC01UnboundUnboundUnboundUnbound UnboundUnboundUnbound
1b6sD01UnboundUnboundUnboundUnbound UnboundUnboundUnbound
1b6rA02UnboundUnboundUnboundUnbound UnboundUnboundAnalogue:SO4
1b6sA02Bound:_MGUnboundUnboundUnbound UnboundBound:ADPUnbound
1b6sB02Bound:_MGUnboundUnboundUnbound UnboundBound:ADPUnbound
1b6sC02Bound:_MGUnboundUnboundUnbound UnboundBound:ADPUnbound
1b6sD02Bound:_MGUnboundUnboundUnbound UnboundBound:ADPUnbound
1b6rA03UnboundUnboundUnboundUnbound UnboundUnboundUnbound
1b6sA03UnboundUnboundUnboundUnbound UnboundUnboundUnbound
1b6sB03UnboundUnboundUnboundUnbound UnboundUnboundUnbound
1b6sC03UnboundUnboundUnboundUnbound UnboundUnboundUnbound
1b6sD03UnboundUnboundUnboundUnbound UnboundUnboundUnbound

Active-site residues
resource
PDB;1b6s
pdbModified residues
         
1b6rA01 
1b6sA01 
1b6sB01 
1b6sC01 
1b6sD01 
1b6rA02GLU 226;GLU 238(magnesium binding)
1b6sA02GLU 226;GLU 238(magnesium binding)
1b6sB02GLU 226;GLU 238(magnesium binding)
1b6sC02GLU 226;GLU 238(magnesium binding)
1b6sD02GLU 226;GLU 238(magnesium binding)
1b6rA03 
1b6sA03 
1b6sB03 
1b6sC03 
1b6sD03 

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[4]p.15489, p.15491

references
[1]
CommentsCHARACTERIZATION, AND SEQUENCE OF 1-20.
Medline ID92287929
PubMed ID1534690
JournalBiochemistry
Year1992
Volume31
Pages5022-32
AuthorsMeyer E, Leonard NJ, Bhat B, Stubbe J, Smith JM
TitlePurification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.
Related UniProtKBP09029
[2]
PubMed ID7918411
JournalBiochemistry
Year1994
Volume33
Pages11927-34
AuthorsFirestine SM, Poon SW, Mueller EJ, Stubbe J, Davisson VJ
TitleReactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms.
[3]
PubMed ID10074353
JournalBiochemistry
Year1999
Volume38
Pages3012-8
AuthorsMeyer E, Kappock TJ, Osuji C, Stubbe J
TitleEvidence for the direct transfer of the carboxylate of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to generate 4-carboxy-5-aminoimidazole ribonucleotide catalyzed by Escherichia coli PurE, an N5-CAIR mutase.
[4]
CommentsX-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
Medline ID20039871
PubMed ID10569930
JournalBiochemistry
Year1999
Volume38
Pages15480-92
AuthorsThoden JB, Kappock TJ, Stubbe J, Holden HM
TitleThree-dimensional structure of N5-carboxyaminoimidazole ribonucleotide synthetase: a member of the ATP grasp protein superfamily.
Related PDB1b6r,1b6s
Related UniProtKBP09029


createdupdated
2004-03-252009-02-26


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Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)
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