EzCatDB: T00100
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DB codeT00100
CATH domainDomain 13.40.50.1070 : Rossmann fold
Domain 23.40.275.10 : L-fucose Isomerase; Chain A, domain 2Catalytic domain
Domain 33.20.14.10 : L-fucose Isomerase; Chain A, domain 3Catalytic domain
E.C.5.3.1.25
CSA1fui
MACiEM0095


Enzyme Name
UniProtKBKEGG

P69922
Protein nameL-fucose isomeraseL-fucose isomerase
SynonymsEC 5.3.1.25
6-deoxy-L-galactose isomerase
FucIase
RefSeqNP_417282.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_491010.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PfamPF02952 (Fucose_iso_C)
PF07881 (Fucose_iso_N1)
PF07882 (Fucose_iso_N2)
[Graphical view]

KEGG pathways
MAP codePathways
MAP00051Fructose and mannose metabolism

UniProtKB:Accession NumberP69922
Entry nameFUCI_ECOLI
ActivityL-fucose = L-fuculose.
SubunitHomohexamer.
Subcellular locationCytoplasm.
CofactorManganese.

Compound table: links to PDB-related databases & PoSSuM

CofactorsSubstratesProducts
KEGG-idC00034C01019C01721
CompoundManganeseL-FucoseL-Fuculose
Typeheavy metalcarbohydratecarbohydrate
ChEBI18291
35154
2181
17617
PubChem23930
17106
6857362
           
1fuiA01UnboundUnboundUnbound
1fuiB01UnboundUnboundUnbound
1fuiC01UnboundUnboundUnbound
1fuiD01UnboundUnboundUnbound
1fuiE01UnboundUnboundUnbound
1fuiF01UnboundUnboundUnbound
1fuiA02UnboundUnboundUnbound
1fuiB02UnboundUnboundUnbound
1fuiC02UnboundUnboundUnbound
1fuiD02UnboundUnboundUnbound
1fuiE02UnboundUnboundUnbound
1fuiF02UnboundUnboundUnbound
1fuiA03Bound:_MNAnalogue:FOCUnbound
1fuiB03Bound:_MNAnalogue:FOCUnbound
1fuiC03Bound:_MNAnalogue:FOCUnbound
1fuiD03Bound:_MNAnalogue:FOCUnbound
1fuiE03Bound:_MNAnalogue:FOCUnbound
1fuiF03UnboundAnalogue:FOCUnbound

Active-site residues
resource
Swiss-prot;P69922 & literature [7]
pdbCatalytic residuesCofactor-binding residues
          
1fuiA01 
 
1fuiB01 
 
1fuiC01 
 
1fuiD01 
 
1fuiE01 
 
1fuiF01 
 
1fuiA02GLU 337
GLU 337(Manganese binding)
1fuiB02GLU 337
GLU 337(Manganese binding)
1fuiC02GLU 337
GLU 337(Manganese binding)
1fuiD02GLU 337
GLU 337(Manganese binding)
1fuiE02GLU 337
GLU 337(Manganese binding)
1fuiF02GLU 337
GLU 337(Manganese binding)
1fuiA03ASP 361
ASP 361;HIS 528(Manganese binding)
1fuiB03ASP 361
ASP 361;HIS 528(Manganese binding)
1fuiC03ASP 361
ASP 361;HIS 528(Manganese binding)
1fuiD03ASP 361
ASP 361;HIS 528(Manganese binding)
1fuiE03ASP 361
ASP 361;HIS 528(Manganese binding)
1fuiF03ASP 361
ASP 361;HIS 528(Manganese binding)

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[7]Fig.11, p.260-262, p.264-266
[10]Scheme 16, p.9-10
[11]p.101

references
[1]
PubMed ID13319278
JournalJ Biol Chem
Year1956
Volume219
Pages557-68
AuthorsGREEN M, COHEN SS
TitleEnzymatic conversion of L-fucose to L-fuculose.
[2]
JournalMethods Enzymol
Year1966
Volume9
Pages583-5
AuthorsMortlock RP
TitleD-Arabinose isomerase.
[3]
PubMed ID4632320
JournalJ Bacteriol
Year1973
Volume113
Pages687-96
AuthorsBoulter JR, Gielow WO
TitleProperties of D-arabinose isomerase purified from two strains of Escherichia coli.
[4]
JournalAgric Biol Chem
Year1974
Volume38
Pages267-73
AuthorsIzumori K, Yamanaka K
TitlePurification and crystallization of D-arabinose (L-fucose) isomerase from Aerobacter aerogenes by polyethylene glycol.
[5]
JournalAgric Biol Chem
Year1976
Volume40
Pages439-440
AuthorsYamanaka K, Izumori K
TitleInhibition of D-arabinose (L-fucose) isomerase activity by Tris and its analogues.
[6]
JournalAgric Biol Chem
Year1979
Volume43
Pages1993-4
AuthorsIzumori K, Yamanaka K
TitleActivation and inhibition of D-arabinose isomerase of Klebsiella aerogenes by L-histidine.
[7]
CommentsX-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
Medline ID98035055
PubMed ID9367760
JournalJ Mol Biol
Year1997
Volume273
Pages256-68
AuthorsSeemann JE, Schulz GE
TitleStructure and mechanism of L-fucose isomerase from Escherichia coli.
Related PDB1fui
Related UniProtKBP69922
[8]
PubMed ID9485504
JournalProteins
Year1997
VolumeSuppl 1
Pages129-33
AuthorsFlockner H, Domingues FS, Sippl MJ
TitleProtein folds from pair interactions: a blind test in fold recognition.
[9]
PubMed ID10368289
JournalStructure Fold Des
Year1999
Volume7
Pages227-36
AuthorsCastillo RM, Mizuguchi K, Dhanaraj V, Albert A, Blundell TL, Murzin AG
TitleA six-stranded double-psi beta barrel is shared by several protein superfamilies.
[10]
PubMed ID11368170
JournalArch Biochem Biophys
Year2001
Volume387
Pages1-10
AuthorsCreighton DJ, Hamilton DS
TitleBrief history of glyoxalase I and what we have learned about metal ion-dependent, enzyme-catalyzed isomerizations.
[11]
JournalTop Curr Chem
Year2001
Volume215
Pages77-114
AuthorsHausler H, Stutz AE
TitleD-xylose (D-glucose) isomerase and related enzymes in carbohydrate synthesis.
[12]
PubMed ID12645017
JournalChemistry
Year2003
Volume9
Pages1281-95
AuthorsCasanova D, Alemany P, Bofill JM, Alvarez S
TitleShape and symmetry of heptacoordinate transition-metal complexes: structural trends.


createdupdated
2005-07-042009-02-26


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