|
CATH domain | Related DB codes (homologues) |
---|
3.50.50.60 : FAD/NAD(P)-binding domain | M00163,D00015,D00041,D00042,D00045,D00064,D00071,T00004,T00015,T00017,T00025,T00213,T00233,T00242 | 3.90.660.10 : Polyamine Oxidase; Chain A, domain 2 | D00042 |
KEGG pathways | MAP code | Pathways |
---|
MAP00252 | Alanine and aspartate metabolism | MAP00271 | Methionine metabolism | MAP00280 | Valine, leucine and isoleucine degradation | MAP00350 | Tyrosine metabolism | MAP00360 | Phenylalanine metabolism | MAP00380 | Tryptophan metabolism | MAP00400 | Phenylalanine, tyrosine and tryptophan biosynthesis | MAP00950 | Alkaloid biosynthesis I |
UniProtKB:Accession Number | P81382 |
---|
Entry name | OXLA_AGKRH |
---|
Activity | An L-amino acid + H(2)O + O(2) = a 2-oxo acid + NH(3) + H(2)O(2). |
---|
Subunit | Homodimer. |
---|
Subcellular location | Secreted. |
---|
Cofactor | FAD. |
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Compound table: links to PDB-related databases & PoSSuM |
---|
| Cofactors | Substrates | Products |
---|
KEGG-id | C00016 | C00001 | C00007 | C00151 | C00014 | C00027 | C00161 |
---|
Compound | FAD | H2O | O2 | L-Amino acid | NH3 | H2O2 | 2-Oxo acid |
---|
Type | amide group,amine group,aromatic ring (only carbon atom),aromatic ring (with nitrogen atoms),carbohydrate,nucleotide | H2O | others | amino acids | amine group,organic ion | others | carbohydrate,carboxyl group |
---|
ChEBI | 16238
| 15377
| 15379 26689 27140
|
| 16134
| 16240
|
|
---|
PubChem | 643975
| 22247451 962
| 977
|
| 222
| 22326046 784
|
|
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| | | | | | | | | | | | | | |
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1f8rA01 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Analogue:CIT |
---|
1f8rB01 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Analogue:CIT |
---|
1f8rC01 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Analogue:CIT |
---|
1f8rD01 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Analogue:CIT |
---|
1f8sA01 |  |  |  |  |  |  |  | Unbound | | Unbound | Analogue:BE2 1 | Unbound | Unbound | Unbound |
---|
1f8sB01 |  |  |  |  |  |  |  | Unbound | | Unbound | Analogue:BE2 4 | Unbound | Unbound | Unbound |
---|
1f8sC01 |  |  |  |  |  |  |  | Unbound | | Unbound | Analogue:BE2 7 | Unbound | Unbound | Unbound |
---|
1f8sD01 |  |  |  |  |  |  |  | Unbound | | Unbound | Analogue:BE2 10 | Unbound | Unbound | Unbound |
---|
1f8sE01 |  |  |  |  |  |  |  | Unbound | | Unbound | Analogue:BE2 13 | Unbound | Unbound | Unbound |
---|
1f8sF01 |  |  |  |  |  |  |  | Unbound | | Unbound | Analogue:BE2 16 | Unbound | Unbound | Unbound |
---|
1f8sG01 |  |  |  |  |  |  |  | Unbound | | Unbound | Analogue:BE2 19 | Unbound | Unbound | Unbound |
---|
1f8sH01 |  |  |  |  |  |  |  | Unbound | | Unbound | Analogue:BE2 22 | Unbound | Unbound | Unbound |
---|
1tdkA01 |  |  |  |  |  |  |  | Unbound | | Unbound | Bound:LVG 491 | Unbound | Unbound | Unbound |
---|
1tdnA01 |  |  |  |  |  |  |  | Unbound | | Unbound | Bound:LEU | Unbound | Unbound | Unbound |
---|
1tdoA01 |  |  |  |  |  |  |  | Unbound | | Unbound | Bound:PHE | Unbound | Unbound | Unbound |
---|
1reoA01 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Analogue:CIT |
---|
1f8rA02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8rB02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8rC02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8rD02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sA02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sB02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sC02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sD02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sE02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sF02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sG02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sH02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1tdkA02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1tdnA02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1tdoA02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1reoA02 |  |  |  |  |  |  |  | Bound:FAD | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8rA03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8rB03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8rC03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8rD03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sA03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sB03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sC03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sD03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sE03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sF03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sG03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1f8sH03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1tdkA03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1tdnA03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1tdoA03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1reoA03 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
---|
[5] | Scheme 1, p.4212-4213 | 2 | [7] | Scheme 1 |
| [8] | Scheme 1 |
| [11] | Fig.1, Fig.4, p.23975-23976 |
|
references | [1] |
---|
PubMed ID | 2044840 |
---|
Journal | Int J Biochem |
---|
Year | 1991 |
---|
Volume | 23 |
---|
Pages | 323-7 |
---|
Authors | Tan NH, Saifuddin MN |
---|
Title | Substrate specificity of king cobra (Ophiophagus hannah) venom L-amino acid oxidase. |
---|
[2] |
---|
PubMed ID | 8755511 |
---|
Journal | Proc Natl Acad Sci U S A |
---|
Year | 1996 |
---|
Volume | 93 |
---|
Pages | 7546-51 |
---|
Authors | Raibekas AA, Massey V |
---|
Title | Glycerol-induced development of catalytically active conformation of Crotalus adamanteus L-amino acid oxidase in vitro. |
---|
[3] |
---|
PubMed ID | 10441379 |
---|
Journal | Arch Biochem Biophys |
---|
Year | 1999 |
---|
Volume | 368 |
---|
Pages | 285-90 |
---|
Authors | Souza DH, Eugenio LM, Fletcher JE, Jiang MS, Garratt RC, Oliva G, Selistre-de-Araujo HS |
---|
Title | Isolation and structural characterization of a cytotoxic L-amino acid oxidase from Agkistrodon contortrix laticinctus snake venom: preliminary crystallographic data. |
---|
[4] |
---|
PubMed ID | 11368308 |
---|
Journal | Arch Biochem Biophys |
---|
Year | 2000 |
---|
Volume | 384 |
---|
Pages | 216-26 |
---|
Authors | Ali SA, Stoeva S, Abbasi A, Alam JM, Kayed R, Faigle M, Neumeister B, Voelter W |
---|
Title | Isolation, structural, and functional characterization of an apoptosis-inducing L-amino acid oxidase from leaf-nosed viper (Eristocophis macmahoni) snake venom. |
---|
[5] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), AND CARBOHYDRATE-LINKAGE SITES |
---|
Medline ID | 20402326 |
---|
PubMed ID | 10944103 |
---|
Journal | EMBO J |
---|
Year | 2000 |
---|
Volume | 19 |
---|
Pages | 4204-15 |
---|
Authors | Pawelek PD, Cheah J, Coulombe R, Macheroux P, Ghisla S, Vrielink A |
---|
Title | The structure of L-amino acid oxidase reveals the substrate trajectory into an enantiomerically conserved active site. |
---|
Related PDB | 1f8r,1f8s |
---|
Related UniProtKB | P81382 |
---|
[6] |
---|
PubMed ID | 11341935 |
---|
Journal | Biochim Biophys Acta |
---|
Year | 2001 |
---|
Volume | 1544 |
---|
Pages | 267-77 |
---|
Authors | Takatsuka H, Sakurai Y, Yoshioka A, Kokubo T, Usami Y, Suzuki M, Matsui T, Titani K, Yagi H, Matsumoto M, Fujimura Y |
---|
Title | Molecular characterization of L-amino acid oxidase from Agkistrodon halys blomhoffii with special reference to platelet aggregation. |
---|
[7] |
---|
PubMed ID | 11453999 |
---|
Journal | Eur J Biochem |
---|
Year | 2001 |
---|
Volume | 268 |
---|
Pages | 4044-53 |
---|
Authors | Geyer A, Fitzpatrick TB, Pawelek PD, Kitzing K, Vrielink A, Ghisla S, Macheroux P |
---|
Title | Structure and characterization of the glycan moiety of L-amino-acid oxidase from the Malayan pit viper Calloselasma rhodostoma. |
---|
[8] |
---|
PubMed ID | 11248687 |
---|
Journal | Eur J Biochem |
---|
Year | 2001 |
---|
Volume | 268 |
---|
Pages | 1679-86 |
---|
Authors | MacHeroux P, Seth O, Bollschweiler C, Schwarz M, Kurfurst M, Au LC, Ghisla S |
---|
Title | L-amino-acid oxidase from the Malayan pit viper Calloselasma rhodostoma. Comparative sequence analysis and characterization of active and inactive forms of the enzyme. |
---|
[9] |
---|
PubMed ID | 12031486 |
---|
Journal | Biochim Biophys Acta |
---|
Year | 2002 |
---|
Volume | 1576 |
---|
Pages | 70-80 |
---|
Authors | Chavan SS, Tian W, Hsueh K, Jawaheer D, Gregersen PK, Chu CC |
---|
Title | Characterization of the human homolog of the IL-4 induced gene-1 (Fig1). |
---|
[10] |
---|
Journal | Enzyme Microb Technol |
---|
Year | 2002 |
---|
Volume | 31 |
---|
Pages | 77-87 |
---|
Authors | Geueke B, Hummel W |
---|
Title | A new bacterial L-amino acid oxidase with a broad substrate specificity: purification and characterization. |
---|
[11] |
---|
PubMed ID | 12015330 |
---|
Journal | J Biol Chem |
---|
Year | 2002 |
---|
Volume | 277 |
---|
Pages | 23973-6 |
---|
Authors | Binda C, Mattevi A, Edmondson DE |
---|
Title | Structure-function relationships in flavoenzyme-dependent amine oxidations: a comparison of polyamine oxidase and monoamine oxidase. |
---|
[12] |
---|
PubMed ID | 12175601 |
---|
Journal | Toxicon |
---|
Year | 2002 |
---|
Volume | 40 |
---|
Pages | 659-65 |
---|
Authors | Du XY, Clemetson KJ |
---|
Title | Snake venom L-amino acid oxidases. |
---|
[13] |
---|
PubMed ID | 14636049 |
---|
Journal | Biochemistry |
---|
Year | 2003 |
---|
Volume | 42 |
---|
Pages | 13826-32 |
---|
Authors | Sobrado P, Fitzpatrick PF |
---|
Title | Analysis of the role of the active site residue Arg98 in the flavoprotein tryptophan 2-monooxygenase, a member of the L-amino oxidase family. |
---|
[14] |
---|
PubMed ID | 15103157 |
---|
Journal | Acta Crystallogr D Biol Crystallogr |
---|
Year | 2004 |
---|
Volume | 60 |
---|
Pages | 974-7 |
---|
Authors | Zhang H, Teng M, Niu L, Wang Y, Wang Y, Liu Q, Huang Q, Hao Q, Dong Y, Liu P |
---|
Title | Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom. |
---|
Related PDB | 1tdk,1tdn,1tdo,1reo |
---|
comments | This enzyme catalyzes three distinct reactions (see [7], [8] & [11]): (A) Hydride transfer from amine group to FAD(ox) (B) Exchange of double-bonded atoms (Schiff-base deformation) (C) Hydride transfer from FADH2(red) to O2
|
created | updated |
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2004-06-18 | 2009-02-26 |
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