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UniProtKB:Accession Number | P18956 | P54422 | A9YTT0 | O25743 |
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Entry name | GGT_ECOLI | GGT_BACSU | A9YTT0_BACLI | O25743_HELPY |
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Activity | A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.Glutathione + H2O = L-cysteinylglycine + L-glutamate. | A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.Glutathione + H2O = L-cysteinylglycine + L-glutamate. |
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Subunit | This enzyme consists of two polypeptide chains, which are synthesized in precursor form from a single polypeptide. | This enzyme consists of two polypeptide chains, which are synthesized in precursor form from a single polypeptide. By similarity |
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Subcellular location | Periplasm. | Secreted |
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Cofactor |
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Compound table: links to PDB-related databases & PoSSuM |
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| Substrates | Products | intermediates |
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KEGG-id | C03193 | C00051 | C00045 | C00001 | C00012 | C01419 | C03363 | C00025 | I00189 | I00186 | I00164 | I00188 | I00187 |
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E.C. | 2.3.2.2,3.4.19.13 | 2.3.2.2,3.4.19.13 | 2.3.2.2 | 3.4.19.13 | 2.3.2.2,3.4.19.13 | 2.3.2.2,3.4.19.13 | 2.3.2.2 | 3.4.19.13 | 2.3.2.2,3.4.19.13 | 2.3.2.2,3.4.19.13 | 2.3.2.2,3.4.19.13 | 2.3.2.2 | 3.4.19.13 |
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Compound | (5-L-Glutamyl)-peptide | Glutathione | Amino acid | H2O | Peptide | Cys-Gly | 5-L-Glutamyl amino acid | L-Glutamate | (5-L-Glutamyl)-peptide-NterminalThr-enzyme tetrahedral-transition-state | Gultathione-NterminalThr-enzyme tetrahedral-transition-state | Gamma-glutamyl-NterminalThr-acyl-enzyme intermediate | Gamma-glutamyl-NterminalThr-AA-enzyme tetrahedral-transition-state | Gamma-glutamyl-NterminalThr-enzyme tetrahedral-transition-state |
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Type | amine group,carboxyl group,peptide/protein | amino acids,carboxyl group,peptide/protein,sulfhydryl group | amino acids | H2O | peptide/protein | amide group,amine group,carboxyl group,sulfhydryl group | amide group,amine group,carboxyl group | amino acids,carboxyl group |
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ChEBI |
| 16856
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| 15377
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| 4047 61694
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| 16015
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PubChem |
| 124886 25246407
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| 22247451 962
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| 439498 7098621
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| 33032 44272391 88747398
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| | | | | | | | | | | | | | | | | | | | |
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2dbuA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbuC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbwA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbwC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbxA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbxC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dg5A01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dg5C01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0wA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0wB01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0xA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0xC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0yA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0yC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8iA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8iC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8jA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8jC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8kA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8kC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2v36A01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2v36C01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3a75A01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3a75C01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3whqA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3whrA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3whsA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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4ottA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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4otuA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2nqoA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2nqoC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2qm6A01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2qm6C01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2qmcA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2qmcC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3fnmA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3fnmC01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbuA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbuC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbwA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbwC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbxA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbxC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dg5A02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dg5C02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0wA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0wB02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0xA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0xC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0yA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0yC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8iA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8iC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8jA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8jC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8kA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8kC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2v36A02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2v36C02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3a75A02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3a75C02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3whqA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3whrA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3whsA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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4ottA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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4otuA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2nqoA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2nqoC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2qm6A02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2qm6C02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2qmcA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2qmcC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3fnmA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3fnmC02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbuB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbuD00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbwB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-bound:GGL | Unbound | Unbound |
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2dbwD00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-bound:GGL | Unbound | Unbound |
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2dbxB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Bound:GLU | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dbxD00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Bound:GLU | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dg5B00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Bound:GLU | Unbound | Unbound | Unbound | Unbound | Unbound |
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2dg5D00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Bound:GLU | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0xB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0xD00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0yB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2e0yD00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2z8iB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:AZS |
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2z8iD00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:AZS |
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2z8jB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:AZS | Unbound |
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2z8jD00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:AZS | Unbound |
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2z8kB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:AVN | Unbound | Unbound |
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2z8kD00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:AVN | Unbound | Unbound |
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2v36B00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2v36D00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3a75B00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Bound:GLU | Unbound | Unbound | Unbound | Unbound | Unbound |
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3a75D00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Bound:GLU | Unbound | Unbound | Unbound | Unbound | Unbound |
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3whqB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3whrB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3whsB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:AVN | Unbound | Unbound |
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4ottB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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4otuB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Bound:GLU | Unbound | Unbound | Unbound | Unbound | Unbound |
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2nqoB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2nqoD00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2qm6B00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Bound:GLU | Unbound | Unbound | Unbound | Unbound | Unbound |
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2qm6D00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Bound:GLU | Unbound | Unbound | Unbound | Unbound | Unbound |
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2qmcB00 |  |  |  |  |  |  |  | Unbound | Analogue:GTB | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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2qmcD00 |  |  |  |  |  |  |  | Unbound | Analogue:GTB | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
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3fnmB00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:AVN | Unbound | Unbound |
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3fnmD00 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:AVN | Unbound | Unbound |
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References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
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[10] | p.6473-6474 |
| [12] | Fig.6, p.540 |
|
references | [1] |
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PubMed ID | 7903400 |
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Journal | J Mol Biol |
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Year | 1993 |
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Volume | 234 |
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Pages | 1259-62 |
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Authors | Kumagai H, Nohara S, Suzuki H, Hashimoto W, Yamamoto K, Sakai H, Sakabe K, Fukuyama K, Sakabe N |
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Title | Crystallization and preliminary X-ray analysis of gamma-glutamyltranspeptidase from Escherichia coli K-12. |
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[2] |
---|
PubMed ID | 7818493 |
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Journal | Biochem J |
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Year | 1994 |
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Volume | 304 |
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Pages | 869-76 |
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Authors | Gololobov MYu, Bateman RC Jr |
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Title | gamma-Glutamyltranspeptidase-catalysed acyl-transfer to the added acceptor does not proceed via the ping-pong mechanism. |
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[3] |
---|
PubMed ID | 8864839 |
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Journal | J Biochem |
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Year | 1996 |
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Volume | 120 |
---|
Pages | 26-8 |
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Authors | Sakai H, Sakabe N, Sasaki K, Hashimoto W, Suzuki H, Tachi H, Kumagai H, Sakabe K |
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Title | A preliminary description of the crystal structure of gamma-glutamyltranspeptidase from E. coli K-12. |
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[4] |
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PubMed ID | 12207027 |
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Journal | J Biol Chem |
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Year | 2002 |
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Volume | 277 |
---|
Pages | 43536-43 |
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Authors | Suzuki H, Kumagai H |
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Title | Autocatalytic processing of gamma-glutamyltranspeptidase. |
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[5] |
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PubMed ID | 14516202 |
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Journal | Biochemistry |
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Year | 2003 |
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Volume | 42 |
---|
Pages | 11504-13 |
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Authors | Castonguay R, Lherbet C, Keillor JW |
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Title | Kinetic studies of rat kidney gamma-glutamyltranspeptidase deacylation reveal a general base-catalyzed mechanism. |
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[6] |
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PubMed ID | 12643897 |
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Journal | Bioorg Med Chem Lett |
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Year | 2003 |
---|
Volume | 13 |
---|
Pages | 997-1000 |
---|
Authors | Lherbet C, Morin M, Castonguay R, Keillor JW |
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Title | Synthesis of aza and oxaglutamyl-p-nitroanilide derivatives and their kinetic studies with gamma-glutamyltranspeptidase. |
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[7] |
---|
PubMed ID | 15466585 |
---|
Journal | Appl Environ Microbiol |
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Year | 2004 |
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Volume | 70 |
---|
Pages | 6324-8 |
---|
Authors | Suzuki H, Miwa C, Ishihara S, Kumagai H |
---|
Title | A single amino acid substitution converts gamma-glutamyltranspeptidase to a class IV cephalosporin acylase (glutaryl-7-aminocephalosporanic acid acylase). |
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[8] |
---|
PubMed ID | 16399402 |
---|
Journal | Methods Enzymol |
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Year | 2005 |
---|
Volume | 401 |
---|
Pages | 449-67 |
---|
Authors | Keillor JW, Castonguay R, Lherbet C |
---|
Title | Gamma-glutamyl transpeptidase substrate specificity and catalytic mechanism. |
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[9] |
---|
PubMed ID | 16672227 |
---|
Journal | J Biol Chem |
---|
Year | 2006 |
---|
Volume | 281 |
---|
Pages | 19029-37 |
---|
Authors | Boanca G, Sand A, Barycki JJ |
---|
Title | Uncoupling the enzymatic and autoprocessing activities of Helicobacter pylori gamma-glutamyltranspeptidase. |
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[10] |
---|
PubMed ID | 16618936 |
---|
Journal | Proc Natl Acad Sci U S A |
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Year | 2006 |
---|
Volume | 103 |
---|
Pages | 6471-6 |
---|
Authors | Okada T, Suzuki H, Wada K, Kumagai H, Fukuyama K |
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Title | Crystal structures of gamma-glutamyltranspeptidase from Escherichia coli, a key enzyme in glutathione metabolism, and its reaction intermediate. |
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Related PDB | 2dbu,2dbw,2dbx,2dg5 |
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[11] |
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PubMed ID | 17960917 |
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Journal | Biochemistry |
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Year | 2007 |
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Volume | 46 |
---|
Pages | 13407-14 |
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Authors | Morrow AL, Williams K, Sand A, Boanca G, Barycki JJ |
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Title | Characterization of Helicobacter pylori gamma-glutamyltranspeptidase reveals the molecular basis for substrate specificity and a critical role for the tyrosine 433-containing loop in catalysis. |
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Related PDB | 2qm6,2qmc |
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[12] |
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PubMed ID | 17107958 |
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Journal | J Biol Chem |
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Year | 2007 |
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Volume | 282 |
---|
Pages | 534-41 |
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Authors | Boanca G, Sand A, Okada T, Suzuki H, Kumagai H, Fukuyama K, Barycki JJ |
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Title | Autoprocessing of Helicobacter pylori gamma-glutamyltranspeptidase leads to the formation of a threonine-threonine catalytic dyad. |
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Related PDB | 2nqo |
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[13] |
---|
PubMed ID | 17135273 |
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Journal | J Biol Chem |
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Year | 2007 |
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Volume | 282 |
---|
Pages | 2433-9 |
---|
Authors | Okada T, Suzuki H, Wada K, Kumagai H, Fukuyama K |
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Title | Crystal structure of the gamma-glutamyltranspeptidase precursor protein from Escherichia coli. Structural changes upon autocatalytic processing and implications for the maturation mechanism. |
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Related PDB | 2e0w,2e0x,2e0y |
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[14] |
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PubMed ID | 18036555 |
---|
Journal | Biochem Biophys Res Commun |
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Year | 2008 |
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Volume | 366 |
---|
Pages | 294-300 |
---|
Authors | Ong PL, Yao YF, Weng YM, Hsu WH, Lin LL |
---|
Title | Residues Arg114 and Arg337 are critical for the proper function of Escherichia coli gamma-glutamyltranspeptidase. |
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[15] |
---|
PubMed ID | 18555071 |
---|
Journal | J Mol Biol |
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Year | 2008 |
---|
Volume | 380 |
---|
Pages | 361-72 |
---|
Authors | Wada K, Hiratake J, Irie M, Okada T, Yamada C, Kumagai H, Suzuki H, Fukuyama K |
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Title | Crystal structures of Escherichia coli gamma-glutamyltranspeptidase in complex with azaserine and acivicin: novel mechanistic implication for inhibition by glutamine antagonists. |
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Related PDB | 2z8i,2z8j,2z8k |
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[16] |
---|
PubMed ID | 19256527 |
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Journal | Biochemistry |
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Year | 2009 |
---|
Volume | 48 |
---|
Pages | 2459-67 |
---|
Authors | Williams K, Cullati S, Sand A, Biterova EI, Barycki JJ |
---|
Title | Crystal structure of acivicin-inhibited gamma-glutamyltranspeptidase reveals critical roles for its C-terminus in autoprocessing and catalysis. |
---|
Related PDB | 3fnm |
---|
[17] |
---|
PubMed ID | 19788059 |
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Journal | Indian J Biochem Biophys |
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Year | 2009 |
---|
Volume | 46 |
---|
Pages | 281-8 |
---|
Authors | Hsu WH, Ong PL, Chen SC, Lin LL |
---|
Title | Contribution of Ser463 residue to the enzymatic and autoprocessing activities of Escherichia coli gamma-glutamyltranspeptidase. |
---|
[18] |
---|
PubMed ID | 20572278 |
---|
Journal | Biotechnol J |
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Year | 2010 |
---|
Volume | 5 |
---|
Pages | 829-37 |
---|
Authors | Suzuki H, Yamada C, Kijima K, Ishihara S, Wada K, Fukuyama K, Kumagai H |
---|
Title | Enhancement of glutaryl-7-aminocephalosporanic acid acylase activity of gamma-glutamyltranspeptidase of Bacillus subtilis. |
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[19] |
---|
PubMed ID | 20088880 |
---|
Journal | FEBS J |
---|
Year | 2010 |
---|
Volume | 277 |
---|
Pages | 1000-9 |
---|
Authors | Wada K, Irie M, Suzuki H, Fukuyama K |
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Title | Crystal structure of the halotolerant gamma-glutamyltranspeptidase from Bacillus subtilis in complex with glutamate reveals a unique architecture of the solvent-exposed catalytic pocket. |
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Related PDB | 3a75 |
---|
[20] |
---|
PubMed ID | 21298394 |
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Journal | Extremophiles |
---|
Year | 2011 |
---|
Volume | 15 |
---|
Pages | 259-70 |
---|
Authors | Castellano I, Di Salle A, Merlino A, Rossi M, La Cara F |
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Title | Gene cloning and protein expression of gamma-glutamyltranspeptidases from Thermus thermophilus and Deinococcus radiodurans: comparison of molecular and structural properties with mesophilic counterparts. |
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[21] |
---|
PubMed ID | 22664192 |
---|
Journal | Enzyme Microb Technol |
---|
Year | 2012 |
---|
Volume | 51 |
---|
Pages | 86-94 |
---|
Authors | Hu HY, Yang JC, Chen JH, Chi MC, Lin LL |
---|
Title | Enzymatic characterization of Bacillus licheniformis gamma-glutamyltranspeptidase fused with N-terminally truncated forms of Bacillus sp. TS-23 alpha-amylase. |
---|
[22] |
---|
PubMed ID | 23036908 |
---|
Journal | Biochim Biophys Acta |
---|
Year | 2013 |
---|
Volume | 1834 |
---|
Pages | 149-57 |
---|
Authors | Pica A, Russo Krauss I, Castellano I, La Cara F, Graziano G, Sica F, Merlino A |
---|
Title | Effect of NaCl on the conformational stability of the thermophilic gamma-glutamyltranspeptidase from Geobacillus thermodenitrificans: Implication for globular protein halotolerance. |
---|
[23] |
---|
PubMed ID | 24531494 |
---|
Journal | Acta Crystallogr D Biol Crystallogr |
---|
Year | 2014 |
---|
Volume | 70 |
---|
Pages | 607-14 |
---|
Authors | Ida T, Suzuki H, Fukuyama K, Hiratake J, Wada K |
---|
Title | Structure of Bacillus subtilis gamma-glutamyltranspeptidase in complex with acivicin: diversity of the binding mode of a classical and electrophilic active-site-directed glutamate analogue. |
---|
Related PDB | 3whq,3whr,3whs |
---|
[24] |
---|
PubMed ID | 24780583 |
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Journal | Biochim Biophys Acta |
---|
Year | 2014 |
---|
Volume | 1844 |
---|
Pages | 1523-9 |
---|
Authors | Lin LL, Chen YY, Chi MC, Merlino A |
---|
Title | Low resolution X-ray structure of gamma-glutamyltranspeptidase from Bacillus licheniformis: opened active site cleft and a cluster of acid residues potentially involved in the recognition of a metal ion. |
---|
Related PDB | 4ott,4otu |
---|
comments | This enzyme belongs to the N-terminal nucleophile (Ntn) hydrolase superfamily (CATH 3.60.20.10) (see [4], [9]). This enzyme consists of one large subunit and one small subunit, which are generated from an inactive precursor protein through an intramolecular autocatalytic processing (or autoprocessing) ([4], [9]). The oxygen atom of the sidechain of the N-terminal residue of the small subunit, threonine, acts as a nucleophile in both the autocatalytic processing and enzymatic reactions ([4], [9]). This residue is invariably conserved throughout all the homologous enzymes. As for a general base, alpha-amino group of the newly generated N-terminal residue on processing acts in the enzymatic reactions in the Ntn hydrolase superfamily, whereas it has not been elucidated for the autoctalytic processing reaction of this enzyme (see [4]). This enzyme catalyzes the hydrolysis of gamma-glutamyl bonds in gamma-glutamyl compounds such as glutathione (EC 3.4.19.13) and the transfer of the gamma-glutamyl group to other amino acids and short peptides (EC 2.3.2.2) (see [3], [5], [8], [9], [10]). According to the literature ([11], [12]), this enzyme catalyzes the hydrolysis reaction as follows: (h1) The N-terminal alpha-amino group of Thr380 (of 2nqo, 2qm6) on the small subunit acts as a general base to enhance the nucleophilicity of the sidechain of Thr380, whereas the sidechain hydroxyl group of Thr398 may modulate the activity of sidechain and the alpha-amino group of Thr380. (Thr398 modulate the alpha-amino group of Thr380 throughout the reaction.) (h2) The activated Thr380 makes a nucleophilic attack on the gamma-glutamyl peptide bond of glutathione, leading to formation of the tetrahedral intermediate (I00186). (h3) The tetrahedral intermediate (I00186) is stabilized by the oxyanion hole composed of the mainchain amide groups of Gly472 and Gly473. (h4) The alpha-amino group of Thr380 acts as a general acid to protonate the leaving group, Cys-Gly portion of the intermedaite (I00186), to facilitate the collapse of the intermediate. This reaction leads to the formation of an acyl enzyme intermediate (I00164). This intermediate is stabilized by the amide group of Gly472. (h5) The alpha-amino group of Thr380 acts as a general base to activate a incoming water molecule. (h6) The activated water makes a nucleophilic attack on the acyl enzyme intermediate (I00164), forming the tetrahedral intermediate (I00187). This intermediate is also stabilized by the oxyanion hole (Gly472 and Gly473). (h7) The alpha-amino group of Thr380 may act as a general acid to protonate its own sidechain, facilitating the release of gamma-glutamyl group as a leaving Glu. By this, the active site is regenerated. Similarly, this enzyme catalyzes the transfer reaction as follows: (t1) The N-terminal alpha-amino group of Thr380 (of 2nqo, 2qm6) on the small subunit acts as a general base to enhance the nucleophilicity of the sidechain of Thr380, whereas the sidechain hydroxyl group of Thr398 may modulate the activity of sidechain and the alpha-amino group of Thr380. (Thr398 modulate the alpha-amino group of Thr380 throughout the reaction.) (t2) The activated Thr380 makes a nucleophilic attack on the gamma-glutamyl peptide bond of glutathione, leading to formation of the tetrahedral intermediate (I00186). (t3) The tetrahedral intermediate (I00186) is stabilized by the oxyanion hole composed of the mainchain amide groups of Gly472 and Gly473. (t4) The alpha-amino group of Thr380 acts as a general acid to protonate the leaving group, Cys-Gly portion of the intermedaite (I00186), to facilitate the collapse of the intermediate. This reaction leads to the formation of an acyl enzyme intermediate (I00164). This intermediate is stabilized by the amide group of Gly472. (t5) The alpha-amino group of Thr380 acts as a general base to activate the amino group of a incoming amino acid. (t6) The activated amino group of the amino acid makes a nucleophilic attack on the acyl enzyme intermediate (I00164), forming the tetrahedral intermediate (I00188). This intermediate is also stabilized by the oxyanion hole (Gly472 and Gly473). (t7) The alpha-amino group of Thr380 may act as a general acid to protonate its own sidechain, facilitating the release of L-glutamyl amino acid as a leaving group. By this, the active site is regenerated. During the autoprocessing, the reaction proceeds similarly to the hydrolysis and transfer reactions, except that the alpha-amino group of Thr380 is not available (see [13]). Instead of the alpha-amino group of Thr380 in the mature enzyme, a water molecule may act as a general base to activate the nucleophile, Thr, in the autoprocessing (see [13]).
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created | updated |
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2013-08-10 | 2018-04-23 |
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